Investigating the role of human papillomavirus in lung cancer

Argyri, Elena; Tsimplaki, Elpida; Marketos, C.; Politis, George D.; Panotopoulou, Efstathia

doi:10.1016/j.pvr.2016.12.002

Cited by 29 publications

(23 citation statements)

References 30 publications

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“…In addition, some recent publications have indicated a positive link between HPV and etiopathogenesis of lung carcinoma, supporting the present findings (26,27). However, others have failed to identify such a causative link (28).…”

Section: Discussionsupporting

confidence: 89%

Human papillomavirus prevalence in lung carcinomas in Bulgaria

Shikova

Ivanova

Alexandrova

et al. 2017

Microbiology and Immunology

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A possible association between high-risk human papillomaviruses (HPV) and lung cancer has been investigated for decades with discrepant results. The aim of this study was to determine the prevalence of HPV16 and 18 in Bulgarian patients with lung cancer. Two hundred and nine biopsy specimens from patients with histologically proven lung cancer and without cancer were analyzed. Each sample was subjected to three parallel PCRs using broad spectrum GP5+/6+ primers and type-specific (TS) primers for HPV types 16 and 18. Of the 132 lung carcinoma samples, 33 (25%) were positive for HPV16 and/or HPV18 by TS PCR whereas only five (3.8%) samples were HPV positive by consensus PCR. All non-malignant controls were HPV negative. HPV18 was the more prevalent, being found in 11.4% of samples, followed by HPV16 in 9.1% samples; 4.5% of lesions were positive for both HPV16 and HPV18. HPV16/18 were most prevalent in small cell carcinoma (29.2%) and least prevalent in squamous cell carcinoma (23.3%). HPV was only detected in squamous cell carcinoma and adenosquamous carcinoma by consensus PCR. This study revealed a high HPV16/18 prevalence in lung carcinoma samples from Bulgarian patients when TS PCR was used to detect them. The difference between HPV positivity as detected by consensus and by TS PCR was significant, indicating the importance of methodological issues in explaining the discrepancies between previous studies. HPV18 was more common than HPV16. No association between HPV16/18 status and histopathological diagnosis was identified.

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Section: Discussionsupporting

confidence: 89%

Human papillomavirus prevalence in lung carcinomas in Bulgaria

Shikova

Ivanova

Alexandrova

et al. 2017

Microbiology and Immunology

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“…Chang et al [37], in addition to the evaluating the presence of HPV DNA, also evaluated the expression of p16 on NSCLC by IHC, and as a result, no correlation between the HPV and p16 expression was determined. Other authors, such as van Boerdonk et al [42], Bishop et al [43] and Doxtader et al [41], presented similar results, with 20 to 30% of NSCLC, both adenocarcinoma and squamous cell carcinoma, positive for p16 and negative for HPV DNA. In addition, these authors concluded Stage I -II 0 (0,0) 6 (10,5) 0,580 III -IV 10 (100,0) 51 (89,5) Total 10 (100) 60 (100) (*) Cases with missing values were excluded from the analysis n number; TNM staging: a system based on the size and/ or the extension of the primary tumor (T), the number of compromised lymph nodes (N) and the presence of metastasis (M).…”

Section: Discussionmentioning

confidence: 60%

Human papillomavirus is not associated to non-small cell lung cancer: data from a prospective cross-sectional study

Silva

Mariano

Pastrez

et al. 2019

Infect Agents Cancer

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Background: The pathogenesis of lung cancer is triggered by a combination of genetic and environmental factors, being the tobacco smoke the most important risk factor. Nevertheless, the incidence of lung cancer in non-smokers is gradually increasing, which demands the search for different other etiological factors such as occupational exposure, previous lung disease, diet among others. In the early 80's a theory linked specific types of human papillomavirus (HPV) to lung cancer due to morphological similarities of a subset of bronchial squamous cell carcinomas with other HPV-induced cancers. Since then, several studies revealed variable rates of HPV DNA detection. The current study aimed to provide accurate information on the prevalence of HPV DNA in lung cancer.Methods: Biopsies were collected from 77 newly diagnosed non-small cell lung cancer (NSCLC) patients treated at the Thoracic Oncology Department at Barretos Cancer Hospital. The samples were formalin fixed and paraffin embedded (FFPE), histologic analysis was performed by an experienced pathologist. DNA was extracted from FFPE material using a commercial extraction kit and HPV DNA detection was evaluated by multiplex PCR and HPV16 specific real-time PCR. Results: HPV was not identified in any of the samples analysed (69). Conclusions:Our data demonstrated a lack of HPV DNA in a series of NSCL cancers.

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“…Our results indicate that the stringent contamination precautions we took while conducting pre-PCR and PCR activities were sufficient for avoiding contamination. Moreover, the presence of HPV DNA does not signify an active HPV infection, and samples found positive for HPV DNA are frequently negative for HPV transcripts 14,25 . The measurement of viral E6/ E7 transcripts is widely considered the gold standard for detecting biologically-relevant HPV infection.…”

Section: Discussionmentioning

confidence: 99%

The absence of high-risk human papillomavirus in Czech non-small cell lung cancer cases

Jaworek

Koudeláková

Slavkovský

et al. 2020

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Aims. The purpose of our study was to examine the presence of human papillomavirus (HPV) DNA in Czech patients with non-small cell lung cancer (NSCLC). Methods. A highly sensitive quantitative polymerase chain reaction (qPCR) detecting the E6 gene of HPV16, 18, 31, and 56 was designed. The limit of detection was assessed using serial dilutions of HPV-positive plasmids. The qPCR was validated on a set of 402 cervical swabs where the qPCR, Cobas, and PapilloCheck methods were tested in parallel. Finally, qPCR was used for HPV detection in a set of 80 patients with primary NSCLC, both from formalin-fixed paraffinembedded (FFPE) and fresh frozen (FF) tissue samples. Results. The qPCR method was able to reliably detect at least 4 copies of the E6 gene per reaction in HPV16, 18, and 31, and 40 copies per reaction in HPV56. The sensitivity and specificity of the qPCR were 75.6-99.3% and 63.9-100% respectively, depending on the HPV genotype and reference method used. HPV DNA was not detected in the FFPE and FF samples from the set of 80 NSCLC patients. Conclusion. No hrHPV DNA was found in primary NSCLC tumors from a Czech population.

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Investigating the role of human papillomavirus in lung cancer

Cited by 29 publications

References 30 publications

Human papillomavirus prevalence in lung carcinomas in Bulgaria

Human papillomavirus prevalence in lung carcinomas in Bulgaria

Human papillomavirus is not associated to non-small cell lung cancer: data from a prospective cross-sectional study

The absence of high-risk human papillomavirus in Czech non-small cell lung cancer cases

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