Investigating the role of metals loaded on nitrogen-doped carbon-nanotube electrodes in electroenzymatic alcohol dehydrogenation

Kondaveeti, Sanath; Park, Gi Dae; Shanmugam, Ramasamy; Pagolu, Raviteja; Patel, Sanjay; Bisht, Aarti; Kim, Dong Rip; Kang, Yun Chan; Lee, Jung-Kul

doi:10.1016/j.apcatb.2022.121195

Cited by 16 publications

(3 citation statements)

References 47 publications

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“…An activity increase of 32.84, 36.85, 42.54, 29.58, 18.59, 45.99, and 47.01% was observed in the presence of 5 mM concentration of Na + , K + , Mn 2+ , Fe 2+ , Mg 2+ , Cu 2+ , and Ca 2+ , respectively. Such an outcome suggests the requirement of these metal ions for optimal activity of the enzyme (Patel et al, 2019 , 2022 ; Kondaveeti et al, 2022 ). Besides, slight inhibition with 86.24% of keratinase activity was observed at low concentration (2 mM) while a slight increase of 10.32% in activity was testified at high concentration (5 mM) of metal ion Co 2+ .…”

Section: Resultsmentioning

confidence: 99%

Chicken Feather Waste Valorization Into Nutritive Protein Hydrolysate: Role of Novel Thermostable Keratinase From Bacillus pacificus RSA27

et al. 2022

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Microbial keratinases exhibit a momentous role in converting keratin biowastes into exceedingly valuable protein supplements. This study reports a novel, highly stable keratinase from Bacillus pacificus RSA27 for the production of pure peptides rich in essential amino acids from chicken feathers. Purified keratinase showed a specific activity of 38.73 U/mg, 2.58-fold purification, and molecular weight of 36 kDa. Kinetic studies using a chicken feather as substrate report Km and Vmax values of 5.69 mg/ml and 142.40 μg/ml/min, respectively, suggesting significant enzyme-substrate affinity/biocatalysis. Identification and in silico structural-functional analysis of keratinase discovered the presence of distinct amino acid residues and their positions. Besides, keratinase possesses a high-affinity calcium-binding site (Asp128, Leu162, Asn164, Ile166, and Val168) and a catalytic triad of Asp119, His151, and Ser308, known attributes of serine protease (subtilisin family). Furthermore, a scale-up to 5 L fermenter revealed complete feather hydrolysis (94.5%) within 24 h with high activity (789 U/ml) and total amino acid of 153.97 μmol/ml. Finally, cytotoxicity evaluation of protein hydrolysate resulted in negligible cytotoxic effects (1.02%) on the mammalian hepatoblastoma cell line, signifying its potential biotechnological applications.

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Section: Resultsmentioning

confidence: 99%

Chicken Feather Waste Valorization Into Nutritive Protein Hydrolysate: Role of Novel Thermostable Keratinase From Bacillus pacificus RSA27

et al. 2022

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“…In BES, electroactive bacterial consortia or strains residing on the electrodes acts as the biocatalyst for the degradation of organic matters present in wastewater, which generates electron equivalents required for anodic oxidation and cathodic reduction reactions [1][2][3]. In some BES systems, enzymes acts as biocatalysts to produce electrochemical energy from the simple organic molecules which are distinctly called as enzymatic biofuel cells [4][5][6]. BES emerged into several applications such as microbial fuel cells (MFCs) for bioelectricity (bioelectrogenesis), microbial electrolysis cells (MECs) for biohydrogen production, microbial desalination cells (MDCs) for water desalination and microbial electrosynthesis (MES) to produce the bio-chemicals [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Sustainable bioelectrochemical systems for bioenergy generation via waste treatment from petroleum industries

Kondaveeti

Govindarajan

Mohanakrishna

et al. 2023

Fuel

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“…On the other hand, lanthanide-doped nanoparticles can generate upconversion luminescence under the excitation of an external excitation light source [ 10 ]. Therefore, noninvasive, non-contact biological tissue temperature measurements based on lanthanide-doped nanoparticles are possible [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Multi-Mode Lanthanide-Doped Ratiometric Luminescent Nanothermometer for Near-Infrared Imaging within Biological Windows

Heydari

et al. 2023

Nanomaterials

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Owing to its high reliability and accuracy, the ratiometric luminescent thermometer can provide non-contact and fast temperature measurements. In particular, the nanomaterials doped with lanthanide ions can achieve multi-mode luminescence and temperature measurement by modifying the type of doped ions and excitation light source. The better penetration of the near-infrared (NIR) photons can assist bio-imaging and replace thermal vision cameras for photothermal imaging. In this work, we prepared core–shell cubic phase nanomaterials doped with lanthanide ions, with Ba2LuF7 doped with Er3+/Yb3+/Nd3+ as the core and Ba2LaF7 as the coating shell. The nanoparticles were designed according to the passivation layer to reduce the surface energy loss and enhance the emission intensity. Green upconversion luminescence can be observed under both 980 nm and 808 nm excitation. A single and strong emission band can be obtained under 980 nm excitation, while abundant and weak emission bands appear under 808 nm excitation. Meanwhile, multi-mode ratiometric optical thermometers were achieved by selecting different emission peaks in the NIR window under 808 nm excitation for non-contact temperature measurement at different tissue depths. The results suggest that our core–shell NIR nanoparticles can be used to assist bio-imaging and record temperature for biomedicine.

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Investigating the role of metals loaded on nitrogen-doped carbon-nanotube electrodes in electroenzymatic alcohol dehydrogenation

Cited by 16 publications

References 47 publications

Chicken Feather Waste Valorization Into Nutritive Protein Hydrolysate: Role of Novel Thermostable Keratinase From Bacillus pacificus RSA27

Chicken Feather Waste Valorization Into Nutritive Protein Hydrolysate: Role of Novel Thermostable Keratinase From Bacillus pacificus RSA27

Sustainable bioelectrochemical systems for bioenergy generation via waste treatment from petroleum industries

Multi-Mode Lanthanide-Doped Ratiometric Luminescent Nanothermometer for Near-Infrared Imaging within Biological Windows

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