Investigating the underlying mechanism of Saccharomyces cerevisiae in response to ethanol stress employing RNA-seq analysis

Abstract

Saccharomyces cerevisiae has been widely used for wine fermentation and bio-fuels production. A S. cerevisiae strain Sc131 isolated from tropical fruit shows good fermentation properties and ethanol tolerance, exhibiting significant potential in Chinese bayberry wine fermentation. In this study, RNA-sequence and RT-qPCR was used to investigate the transcriptome profile of Sc131 in response to ethanol stress. Scanning Electron Microscopy were carried out to observe surface morphology of yeast cells. Totally, 937 genes were identified differential expressed, including 587 up-regulated and 350 down-regulated genes, after 4-h ethanol stress (10% v/v). Transcriptomic analysis revealed that, most genes involved in regulating filamentous growth or pseudohyphal growth were significantly up-regulated in response to ethanol stress. The complex protein quality control machineries, Hsp90/Hsp70 and Hsp104/Hsp70/Hsp40 based chaperone system combining with ubiquitin-proteasome proteolytic pathway were both activated to recognize and degrade misfolding proteins. Genes related to biosynthesis and metabolism of two well-known stress-responsive substances trehalose and ergosterol were generally up-regulated, while genes associated with amino acids biosynthesis and metabolism processes were differentially expressed. Moreover, thiamine was also important in response to ethanol stress. This research may promote the potential applications of Sc131 in the fermentation of Chinese bayberry wine.

Reprogramming of the Ethanol Stress Response in Saccharomyces cerevisiae by the Transcription Factor Znf1 and Its Effect on the Biosynthesis of Glycerol and Ethanol

“…5D ). According to Li et al ( 63 ), HSP104 (a member of the protein quality control complex), together with the ubiquitin-proteasome proteolytic pathway, is required for protein disaggregation and degradation of misfolded proteins under ethanol stress, among others ( 63 ). Additionally, protein disaggregase Hsp104 is mediated, propagated, and transmitted efficiently to newly formed buds ( 64 ).…”

Thiamine leads to oxidative stress resistance via regulation of the glucose metabolism

“…In the study conducted by Li et al (23) has shown that upregulation of the genes involved in thiamine and thiamine pirophosphate (TPP) biosynthetic processes are important against ethanol stress in S. cerevisiae. In the present study, after the effective thiamine concentration was determined by using H 2 O 2 -induced oxidative stress (Figure 1b), we examined the effect of 1.5 µM thiamine on cell viability under osmotic and heat stresses, which were triggered with sorbitol and high-temperature, respectively.…”

Homology‐ and cross‐resistance of Lactobacillus plantarum to acid and osmotic stress and the influence of induction conditions on its proliferation by RNA‐Seq

“…RNA concentration was measured by Qubit® RNA Assay Kit in Qubit® 2.0. And RNA integrity and purity were evaluated using the RNA Nano 6000 Assay Kit and the NanoPhotometer® spectrophotometer (Implen) [20]. The construction of the libraries and the RNA-Seq were performed by LC-Bio Technology Co., Ltd.…”