Investigating thermal stability based on the structural changes of lactase enzyme by several orthogonal methods

Király, Márton; Kiss, Borbála Dalmadi; Horváth, Péter; Drahos, László; Mirzahosseini, Arash; Pálfy, Gyula; Antal, István; Ludányi, Krisztina

doi:10.1016/j.btre.2021.e00637

Cited by 4 publications

(2 citation statements)

References 32 publications

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“…Upon completion of manufacture, the activity of the finished dosage forms was compared to the starting material. In all cases, the end products retained more than 98% (Matrix: 98.22 ± 1.71%, Layered: 98.59 ± 0.86%, n = 10-10), of their original activity, confirming that the appropriate production conditions were chosen according to our preliminary measurements [27].…”

Section: Physical Characterization Of Particlessupporting

confidence: 61%

Development and Dissolution Study of a β-Galactosidase Containing Drinking Straw

et al. 2022

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Today, in addition to many different physicochemical and pharmacological properties of the active ingredients and excipients, the developer of a pharmaceutical formulation must take into account several factors during the formulation process in order for the patient to cooperate to use the formulation accurately. One of the innovative solutions in paediatrics may be the use of medicated drinking straws. For our studies, we successfully prepared lactase-containing, rapid disintegration particles by two techniques commonly used in the pharmaceutical industry. The simulation of the usage of the filled straws was presented from a new perspective for the patient by an in vitro method. The effect of the temperature of the liquid used during the administration of the straw and the effect of the frequency during the application on the dissolution rate were investigated. According to our results, in the case of a straw containing rapidly dissolving particles, the temperature of the used liquid and the mode of administration (frequency) play a significant role in the release rate from the composition.

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Section: Physical Characterization Of Particlessupporting

confidence: 61%

Development and Dissolution Study of a β-Galactosidase Containing Drinking Straw

et al. 2022

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“…Quantitative mass spectrometric analysis of the glycans shows a significant difference for some sugar moieties due to high temperature. We can assume that structural changes in the higher order structural levels of the protein or within the glycosylation structures might be responsible for decrease of activity [4].…”

Section: Resultsmentioning

confidence: 99%

Investigating the Influence of Heat Stress to the Activity And Structural Changes of Lactase Enzyme

Király

Kiss

Horváth

et al. 2021

APH

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Chitosan-co-GLU/eucalyptus residue composite membrane for the stabilization of β-d-galactosidase in aqueous solutions

da Cruz,

Polizeli,

Enzweiler

et al. 2023

Polym. Bull.

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Investigating thermal stability based on the structural changes of lactase enzyme by several orthogonal methods

Abstract: Highlights The primary and secondary structure of lactase was not changed by thermal stress. The glycan structure of lactase showed significant alterations after heat stress. Changes in the glycosylation structure affected physico-chemical properties of lactase.

Cited by 4 publications

References 32 publications

Development and Dissolution Study of a β-Galactosidase Containing Drinking Straw

Development and Dissolution Study of a β-Galactosidase Containing Drinking Straw

Investigating the Influence of Heat Stress to the Activity And Structural Changes of Lactase Enzyme

Chitosan-co-GLU/eucalyptus residue composite membrane for the stabilization of β-d-galactosidase in aqueous solutions

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