INVESTIGATION AND ALLEVIATION OF MUTUAL INTERFERENCE OF Fe2+AND PO43-IONS BY PROTONATION

Abstract

Mutual interference of iron and phosphates in the spectrophotometric estimation process is a result of the strong bond in the iron phosphate complex. The interference of these two ions can be successfully overcome by the isolation of the two ions. The present investigation was carried to study the mutual interferences of iron and phosphates in the spectrophotometric analysis. Complexing both Fe and PO4 ions in the form of Fe-P complex were done for isolating the Iron and phosphate radicals from other interferences. Whereas to avoid the interference of Fe in PO4 estimation, AlPO4 complex was formed. This triggered the idea to pre-concentrate the two ions by protonation using a strong acid. The results obtained justified the need for isolation of Fe 2+ and PO4 3-.This study provides an exact estimation of Fe 2+ at pH 1.5. The results obtained were statistically correlated by Pearson's correlation coefficient and the results were statistically significant at p value 0.01.

EFFECT OF pH AND ANION INTERFERENCES ON DETERMINATION OF ORTHOPHOSPHATE SPECIATION BY DIFFUSIVE GRADIENT IN THIN FILM (DGT) TECHNIQUE

“…The speciation of orthophosphate in the aquatic environment as PO4 3-, HPO4 2-, and H2PO4and it available for the organism in aquatic sistem 3 . There are some of the methods for determination of phosphate such as colorimetric (CM) methods with spectrophotometric 4 , ion chromatography (IC) 5 , high-performance liquid chromatography (HPLC) 6 , and inductively coupled plasma (ICP) technique. The detected of phosphorus is usually as orthophosphate 7 .…”