Investigation and comparison of biological effects of regioselectively synthesized thiazole derivatives

Abstract

In this study, anticancer, antibacterial (against hospital-isolated antibioticresistant Escherichia coli strains), antifungal, and antioxidant effects of synthesized heterocyclic compounds 5 and 7 containing thiazole core were examined. Cytotoxicity testing was utilized against MCF-7 breast cancer cells via MTT cell viability assay. Antibacterial and antifungal activities were checked out according to Clinical and Laboratory Standards Institute (CLSI) guidelines, and antioxidant properties were evaluated through scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. Results showed the viability of breast cancer cell lines was reliant on concentration of heterocycles and time of incubation. Synthetic compounds exhibited excellent antibacterial and antifungal properties base on their minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) values as well as high antioxidant activities according to their IC 50 values. Higher anticancer and antibacterial properties were observed with compound 7; on the contrary, thiazole 5 had better antioxidant effects. They can be introduced as potent antimicrobial, antioxidant, and anticancer agents.

RETRACTED: Synthesis of Bioactive Yttrium-Metal–Organic Framework as Efficient Nanocatalyst in Synthesis of Novel Pyrazolopyranopyrimidine Derivatives and Evaluation of Anticancer Activity

“…the cells were treated with concentrations of 6.25 μM/ml, 12.5 μM/ml, 25 μM/ml, 75 μM/ml, 150 μM/ml, and 300 μM/ml of Y-MOF and pyrazolopyranopyrimidines derivatives for 48 h. After 48 h, the media from the well were removed and added 150 μL of fresh media plus 50 μL of MTT solutions (prepared as 2 mg/ml in PBS) and for 4 h were incubated. Finally, MTT solutions were removed and DMSO (200 μL) were added to each well and by spectrophotometer (BioTek Instruments, Inc., Bad Friedrichshall, Germany) the absorbance at 570 nm was read ( Heidari Majd et al, 2017 ; Moghaddam‐manesh et al, 2021 ; Moghaddam‐Manesh and Hosseinzadegan, 2021 ).…”

RETRACTED: An Efficient Ultrasound-Assisted Synthesis of Cu/Zn Hybrid MOF Nanostructures With High Microbial Strain Performance

“…According to previous studies and CLSI (Clinical and Laboratory Standards Institute) guidelines M07-A9, M27-A2, and M26-A, broth micro dilution susceptibility and time-kill tests based on MIC (Minimum Inhibitory Concentration), MBC (minimum bactericidal concentration), and MFC (minimum fungicidal concentration) values were evaluated ( Hosseinzadegan et al, 2020 ; Moghaddam-Manesh et al, 2020 ; Moghaddam-manesh et al, 2021 ).…”

Porous Cu-MOF nanostructures with anticancer properties prepared by a controllable ultrasound-assisted reverse micelle synthesis of Cu-MOF

“…Anticancer activity of Cu-MOF was evaluated using MCF-7 breast cancer cells and the MTT cell viability assay according previously reported methods [ 26 , 27 ]. MCF-7 cells were isolated from the pleural effusion of a 58-year-old woman with metastatic disease.…”