Investigation and Histopathological Evaluation of the Effects of Omeprazole on the Ischemia-Reperfusion Induced Oxidative Damage and DNA Mutation in Rat Ovarian Tissue

Abstract

Ovarian ischemia is usually caused by ovarian torsion, which leads to the obstruction of the ovarian artery and the ovarian vein. Ovarian ischemia is an emergency with an indication for acute intervention 1,2 . Though it can be encountered at all ages in women, it is more frequently seen in the premenarche and in the reproductive period 3 . In the case of late diagnosis and clinical necrosis, ovariectomy is the treatment of choice 4 . However, in children, in order to preserve the ovaries, even in cases with severe impairment of the circulation, it is advised to reperfuse the ovaries by means of detorsion 5-7 .However, contrary to the expectations of tissue perfusion, the reperfusion aggravates the effects of the ischemic damage 8 . This phenomenon, known as reperfusion damage, is initiated by the formation of free oxygen radicals 9 . For this reason, free oxygen radicals are known as the mediators of the reperfusion damage 10 . The mediators of the reperfusion damage (the free oxygen radicals) oxidate the cell membrane lipids and cause In this study, the biochemical and histopathological effects of omeprazole on ischemia-reperfusion (I/R) induced oxidative damage in rat ovarian tissue were investigated. The moment that the animals remained motionless in supine position was considered the appropriate time to perform surgery. The ovaries of the rats were reached through a 2.0-2.5 cm long vertical incision in the lower abdomen. Subsequently, in the omeprazole (OIR) and the control groups (I/RC), a vascular clip was placed in the lower part of the right ovary (the part where the ovary is attached to the uterus) and ischemia was maintained for 3 h. (No ischemia was applied in the healthy group.) After this period, the vascular clip was removed in order to provide reperfusion for 2 h. Afterwards, all the animals were terminated by high dose-anesthesia, the ovaries were removed and histopathological and biochemical studies were performed. Omeprazole has an antioxidant effect and it can have a protective function in the oxidative damage induced by ischemia-reperfusion. We have found that omeprazole prevents oxidative damage due to ischemia-reperfusion injury in rat ovarian tissue.

The effect of metamizole on ischemia/reperfusion injury in the rat ovary: An analysis of biochemistry, molecular gene expression, and histopathology

“…obtained similar histopathological findings to ours in rat ovarian tissue applied to I/R. [ 15 ] Zimmerman and Granger reported that degree of reperfusion injury was associated with the degree of neutrophil activation and tissue infiltration. [ 16 ] This information from the literature supports the findings of this study.…”