Investigation and Optimization of Reactivation of Urease Biosensor for Heavy Metals Inhibition Analysis

Budnikov, S R; Солдаткін, О. О.; Кукла, А. Л.; Khomenko, Iuliia; Dzyadevych, S. V.; Солдаткін, О. П.

doi:10.15407/biotech8.06.016

Cited by 2 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Each enzyme has a specific inhibitor. The inhibition of this specific enzymatic system has been applied as an analytical method to detect toxic pollutants such as heavy metals (Budnikov et al 2015); (Ahmad, 2013); (Kaur, Kumar, & Verma, 2014); (Do, Lin, & Ohara 2011) and pesticides (Gan, Yang, Xie, Wu, & Wen, 2010); (Braham, Barhoumi, & Maaref, 2013).…”

Section: Introductionmentioning

confidence: 99%

Determination of Cu and Pb concentrations based on urease activity inhibition of Durio zibethinus L. seeds

Zusfahair

Fatoni

Ningsih

et al. 2021

Molekul

View full text Add to dashboard Cite

The determination of heavy metal concentrations has been carried out using sophisticated instruments, and therefore a simple and reliable alternative method is needed as a comparison. The study aimed to determine Cu and Pb concentration of standard solution using the urease activity inhibition method of Durio zibethinus L. seeds. The research started with urease extraction from Durio D. zibethinus L. seeds. The activity of the obtained extract was determined using the Nessler method. The optimum substrate concentration was also determined. Urease activity inhibition was carried out using various metal solution concentrations, which continued by plotting a log graph of urea concentration vs. %inhibition. The obtained graph would then determine the metal concentration in a synthetic water sample. The data was then compared to the measurement, determined by the Atomic Absorption Spectrophotometry (AAS) method. Results of the study showed that the urease activity of D. zibethinus L.seeds was 296.774 U/mL. Urease activity was optimum at a urea concentration of 0.3 M. The comparison Cu, and Pb concentration determination using the urease inhibitory activity and AAS methods showed no significant difference at 95% confidence level. This research showed that urease of D. zibethinus L. seed could be used to determine Cu and Pb's concentration based on its inhibiting activity.

show abstract

Section: Introductionmentioning

confidence: 99%

Determination of Cu and Pb concentrations based on urease activity inhibition of Durio zibethinus L. seeds

Zusfahair

Fatoni

Ningsih

et al. 2021

Molekul

View full text Add to dashboard Cite

show abstract

“…Enzymes as catalysts make excellent analytical reagents due to their selectivity and specificity and are frequently used to determine the concentration of their substrates. On the other hand, inhibition of specific enzymatic systems is also being applied as analytical methods for the detection of toxic pollutants such as pesticides [1,2] herbicides [3] and heavy metals [4,5]. Heavy metal ions constitute a serious environmental problem due to their ubiquitous and non-biodegradable nature.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Metal - Induced Inhibition of Soybean Urease as a Tool for Measuring Heavy Metals in Aqueous Samples

Magomya¹,

Barminas²,

Osemeahon³

2017

IOSR JAC

View full text Add to dashboard Cite

show abstract

Investigation and Optimization of Reactivation of Urease Biosensor for Heavy Metals Inhibition Analysis

Cited by 2 publications

References 17 publications

Determination of Cu and Pb concentrations based on urease activity inhibition of Durio zibethinus L. seeds

Determination of Cu and Pb concentrations based on urease activity inhibition of Durio zibethinus L. seeds

Assessment of Metal - Induced Inhibition of Soybean Urease as a Tool for Measuring Heavy Metals in Aqueous Samples

Contact Info

Product

Resources

About