Investigation into the effect of high concentrations of volatile fatty acids in anaerobic digestion on methanogenic communities

Franke‐Whittle, Ingrid H.; Walter, Andreas; Ebner, Christian; Insam, Heribert

doi:10.1016/j.wasman.2014.07.020

Cited by 420 publications

(191 citation statements)

References 64 publications

Supporting

Mentioning

151

Contrasting

Unclassified

Order By: Relevance

“…In our research, band D-05, with a DNA sequence affiliated with Methanosaeta concilii (AB679168), was found in both R-ZM and R-MS throughout the experiments. This was probably due to the low ammonium nitrogen concentration, which did not exceed 166 mg NH 4 + L −1 in both digesters. M. concilii was found to be the most ammonium-sensitive methanogen among a group of pure cultures; it was completely inhibited at a concentration of 560 mg (total) NH 4 + L − 1 at a suboptimal pH of 7.0.…”

Section: The Community Structure Of Methanogens In Anaerobic Sludge Amentioning

confidence: 87%

“…These AD intermediates have been shown to build up when methanogenesis is inhibited by coenzyme M during AD of ferulic acid (an intermediate of lignin degradation) [25]. Isovaleric and isobutyric acid are considered to be the most sensitive indicators of digestion imbalance [4,26], and their occurrence in R-MS could have inhibited methanogenic activity. This could also explain why the observed methane yield was 31% lower than the methane yield that theoretically could be produced in these experimental conditions.…”

Section: Vfas Formationmentioning

confidence: 99%

“…Both genera belong to the order Methanosarcinales, which are acetoclastic methanogens that have been reported to be responsible for approximately 70% of the methane produced in biogas reactors [17]. Members of the genus Methanosaeta have been reported to dominate in reactors with low levels of NH 3 and VFAs [4], whereas Methanosarcina have high metabolic versatility and the ability to use acetate, hydrogen, formate, secondary alcohols and methyl compounds as energy sources [32]. In our research, band D-05, with a DNA sequence affiliated with Methanosaeta concilii (AB679168), was found in both R-ZM and R-MS throughout the experiments.…”

Section: The Community Structure Of Methanogens In Anaerobic Sludge Amentioning

confidence: 99%

“…Franke-Whittle et al [4] found that, when a mixture of cow manure (46%), corn silage (36%), vegetable waste (9%) and potato (9%) was digested under mesophilic conditions, the quantity of Methanosarcina correlated positively with the concentrations of all VFAs, whereas the abundance of Methanobacterium was not affected by VFA concentration. However, when a mixture of cow manure (52%) and food waste (48%) was digested in thermophilic conditions, the abundance of Methanosarcina only had a significant positive correlation with the concentrations of acetate, butyrate and isovalerate, while Methanobacterium abundance had a significant negative correlation with the same VFAs.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effect of Individual Components of Lignocellulosic Biomass on Methane Production and Methanogen Community Structure

Pokój

Klimiuk

Bułkowska

et al. 2018

Waste Biomass Valor

View full text Add to dashboard Cite

One of the major factors that influences the economic feasibility of biogas production is the availability of digestible feedstocks. There is little research on the influence of the chemical composition of biomass on biogas synthesis, especially with regard to the content of lignocellulosic materials. Therefore, the aim of this study was to estimate how differences in the content of cellulose and lignin in lignocellulosic biomass influence the concentrations of individual volatile fatty acids (VFAs) and biogas production. Additionally, the structure of the methanogenic community was examined. The removal of fibrous and non-fibrous materials, the concentrations of individual VFAs, methane production and methanogen community structure were examined during digestion of Zea mays L. and Miscanthus sacchariflorus silages. Organics were removed with higher efficiency during the digestion of Z. mays silage than during digestion of M. sacchariflorus. This was due to the higher non-fibrous carbohydrates content in Z. mays than in M. sacchariflorus. In both digesters, propionate predominated throughout experiment. The methanogenic community in the digester fed with Z. mays was more diverse than that in the digester with M. sacchariflorus. Analysis of 16S rRNA sequences showed that six acetoclastic and four hydrogenotrophic methanogens were present in the digester fed with Z. mays L., while five acetoclastic and three hydrogenotrophic methanogens were in the digester fed with M. sacchariflorus. The abundance of Methanosarcina correlated significantly with the concentration of all analyzed VFAs.Keywords Crop silage · Anaerobic digestion · Syntrophic volatile fatty acids oxidation · Archaeal community structure · Methane yield Statement of NoveltyTo introduce novel biomass types for biogas production, an integrated evaluation approach has to be applied. The present study delivers a combination of technological and microbial data about the process of anaerobic digestion of the commonly used substrates Zea mays L. silage and Miscanthus sacchariflorus silage, the latter of which has a high content of lignin and cellulose. The correlations between the abundance of methanogenic microorganisms and the concentrations of individual VFAs produced during digestion of these substrates were estimated. The results reveal novel associations between the type of substrate used, the rate of methane production and the structure of the methanogenic community. The use of materials with a high lignin content limits biodiversity in the anaerobic digesters, which can result in decreases in anaerobic digestion performance and biogas production.

show abstract

Section: The Community Structure Of Methanogens In Anaerobic Sludge Amentioning

confidence: 87%

Section: Vfas Formationmentioning

confidence: 99%

Section: The Community Structure Of Methanogens In Anaerobic Sludge Amentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Individual Components of Lignocellulosic Biomass on Methane Production and Methanogen Community Structure

Pokój

Klimiuk

Bułkowska

et al. 2018

Waste Biomass Valor

View full text Add to dashboard Cite

show abstract

“…22,23 For optimal process, the concentration of acetic acid should not be higher than 2 g l -1 and concentration of propionic acid higher than 0,9 g l -1 . Increased concentration of propionic acid is the most significant indication of process inhibition 45 and occurs following the acetic acid accumulation.…”

Section: Mesophilic Processmentioning

confidence: 99%

Influence of Thermal and Bacterial Pretreatment of Microalgae on Biogas Production in Mesophilic and Thermophilic Conditions

Vidmar

Fanedl

Logar

et al. 2017

ACSi

View full text Add to dashboard Cite

Microalgae biomass has a great potential in search for new alternative energy sources. They can be used as a substrate for the biogas production in anaerobic digestion. When using microalgae, the efficiency of this process is hampered due to the resistant cell wall. In order to accelerate the hydrolysis of cell wall and increase the efficiency of biogas production we applied two different pretreatments -biological and thermal under mesophilic and thermophilic conditions. During biological pretreatment we incubated microalgae with anaerobic hydrolytic bacteria Pseudobutyrivibrio xylanivorans Mz5 T . In thermal pretreatment we incubated microalgae at 90 °C. We also tested a combined thermal and biological pretreatment in which we incubated P. xylanivorans Mz5 T with thermally pretreated microalgae. Thermal pretreatment in mesophilic and thermophilic process has increased methane production by 21% and 6%, respectively. Biological pretreatment of microalgae has increased methane production by 13%, but only under thermophilic conditions (pretreatment under mesophilic conditions showed no effect on methane production). Thermal-biological pretreatment increased methane production by 12% under thermophilic conditions and by 6% under mesophilic conditions.

show abstract

The influence of physico‐chemical parameters, substrate concentration, and species variations on the biochemical methane production rates of ten tropical/subtropical grasses

Holder

Persaud

Mota‐Meira

et al. 2018

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

There are several problems faced by small island developing states (SIDS), especially when it comes to waste management and energy production. There is little land space available for dumping and fuel has to be imported in order to meet energy demands. These problems can be mitigated by using grass cuttings from landscaping, which are often dumped, to produced biogas, which can be used as a fuel. In this study the rates of methane production of 10 different tropical and subtropical species of grasses were determined. Based on the results for 20 g/kg samples, the specific rates of gas production ranged from 2.40 ± 0.21 NmL/gFM/day (Zoysia matrella) to 6.52 ± 0.36 NmL/gFM/day (lemon grass). It was also observed that organic dry matter (oDM) loads did not correlate with methane production, suggesting that the oDM content is not necessarily an accurate indicator of a substrate's methane potential, and highlighting areas for further study. The results in this paper provide a database on the rates of methane production of grasses which are common to the tropical and subtropical regions and can be found in several SIDS. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd.

show abstract

Investigation into the effect of high concentrations of volatile fatty acids in anaerobic digestion on methanogenic communities

Abstract: HighlightsDifferent methanogenic communities in mesophilic and thermophilic reactors.High VFA levels do not cause major changes in archaeal communities.Real-time PCR indicated greater diversity than ANAEROCHIP microarray.

Cited by 420 publications

References 64 publications

Effect of Individual Components of Lignocellulosic Biomass on Methane Production and Methanogen Community Structure

Effect of Individual Components of Lignocellulosic Biomass on Methane Production and Methanogen Community Structure

Influence of Thermal and Bacterial Pretreatment of Microalgae on Biogas Production in Mesophilic and Thermophilic Conditions

The influence of physico‐chemical parameters, substrate concentration, and species variations on the biochemical methane production rates of ten tropical/subtropical grasses

Contact Info

Product

Resources

About