Investigation into the Elastic Properties of ex vivo Porcine Corneas Subjected to Inflation Test after Cross-Linking Treatment

Matteoli, Sara; Virga, Antonio; Paladini, Iacopo; Mencucci, Rita

doi:10.5301/jabfm.5000262

Cited by 15 publications

(24 citation statements)

References 39 publications

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“…The biomechanical properties of the cornea are associated with the development of corneal diseases such as keratoconus, ectasia after refractive surgery, and possible glaucoma progression [ 1 – 4 ]. Hence, there has been a recent surge of interest in assessing corneal biomechanical properties due to potential clinical implications [ 5 – 7 ]. Moreover, the biomechanical properties of the cornea have been proposed to directly affect intraocular pressure (IOP) measurements, especially for normal tension glaucoma [ 8 , 9 ], and are becoming recognized as necessary for the calibration of IOP in different moduli of tonometers [ 10 – 13 ].…”

Section: Introductionmentioning

confidence: 99%

Estimation of the Corneal Young’s ModulusIn VivoBased on a Fluid-Filled Spherical-Shell Model with Scheimpflug Imaging

Shih

Huang

et al. 2017

Journal of Ophthalmology

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Current intraocular pressure (IOP) measurement using air puff could be erroneous without applying proper corrections. Although noncontact tonometry is not considered to be accurate, it is still popularly used by eye clinics. It is thus necessary to extract the correct information from their results. This study proposes a practical approach to correctly measure IOP in vivo. By embedding a new model-based correction to the Corvis® ST, we can extract the corneal Young's modulus from the patient data. This Young's modulus can be used to correct the IOP readings. The tests were applied to 536 right eyes of 536 healthy subjects (228 male and 308 female) between March of 2012 and April of 2016. The tests were applied to patients at the Department of Ophthalmology, National Taiwan University Hospital and the Hung-Chuo Eye Clinics. The statistical analysis showed that the value for the Young's modulus was independent of all the other parameters collected from the Corvis ST, including the corneal thickness and the intraocular pressure. Therefore, it is important to independently measure the Young's modulus instead of depending on the correlation with the other parameters. This study adds the methodology of measuring corneal stiffness in vivo for ophthalmologists' reference in diagnosis.

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Section: Introductionmentioning

confidence: 99%

Estimation of the Corneal Young’s ModulusIn VivoBased on a Fluid-Filled Spherical-Shell Model with Scheimpflug Imaging

Shih

Huang

et al. 2017

Journal of Ophthalmology

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“…The J-shaped stress-strain curve obtained here for the corneal tissue is commonly observed in animal and human corneas stressed through both the tensile and inflation tests (2-3-4-5-6-7-8-9-10-11-12-13-14-15-16). Similarly to other soft and dense biological tissues, cornea can be considered a composite material consisting of a proteoglycan-based matrix reinforced by collagen fibers (36, 37).…”

Section: Discussionmentioning

confidence: 57%

“…The tensile test represents a very popular method to assess the biomechanical properties of cornea. Specifically, rabbit (3-4-5-6-7), bovine (3, 8) and porcine (9-10-11) models have been frequently analyzed. Often, the biomechanical properties of the animal corneal tissue have been compared with those of the human cornea (3, 7, 8).…”

Section: Introductionmentioning

confidence: 99%

Effect of Topical Antiinflammatory Drugs on Mechanical Behavior of Rabbit Cornea

Lepore

Santis

Pagliara

et al. 2017

Journal of Applied Biomaterials & Functional Materials

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BACKGROUND:\ud A variety of antiinflammatory therapies are employed to promote corneal wound healing. The effects of steroidal and nonsteroidal antiinflammatory drugs on the biomechanical properties of rabbit cornea were investigated over time using tensile tests.\ud METHODS:\ud Full-thickness incisions were made and used to analyze the effects of dexamethasone sodium phosphate 0.1% and diclofenac sodium 0.1% on corneal biomechanical properties during wound healing at 7, 14 and 21 days after surgery.\ud RESULTS:\ud The full-thickness incision deeply modified all of the mechanical properties. At 3 weeks after incision, regardless of the drug therapy, the tensile modulus was about 70% of the value for the intact cornea.\ud CONCLUSIONS:\ud Topical treatment with dexamethasone was particularly effective during the first week after surgery; the second week after surgery, a similar result was observed in the corneas treated with diclofenac. Low doses of steroidal and nonsteroidal antiinflammatory drugs would seem to have the potential to improve biomechanical properties only during the early stage of the healing process of the cornea

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“…Zhang and colleagues studied the corneal biomechanical properties after CXL with a uniaxial tensile test [19], and they found that corneal elastic moduli significantly increased after CXL. Matteoli et al measured porcine corneal biomechanical properties with corneal inflation experiments [18] and found that corneal elastic modulus increased after CXL under high IOP, while no significant difference was found within the physiological range of IOP (15-30mmHg).…”

Section: Discussionmentioning

confidence: 99%

“…Numerous studies that used in vitro experiments have reported the variations of corneal biomechanical properties after CXL [16][17][18][19], showing that corneal stiffness increased after CXL. Some researchers have reported the variation of corneal biomechanical parameters provided by Corvis, Steinberg et al find that the first applanation time (A1T) increased and the second applanation time (A2T) decreased 3 months after CXL [20].…”

Section: Introductionmentioning

confidence: 99%

Influence of dextran solution and corneal collagen crosslinking on Corneal Biomechanical Parameters Evaluated by Corvis ST

Xiao

Tian

Zhang

et al. 2021

Preprint

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Purpose: To analyze the influence of dextran solution and corneal collagen crosslinking (CXL) on corneal biomechanical parameters evaluated by Corneal Visualization Scheimpflug Technology (Corvis ST). Materials and Methods: Forty porcine eyes were included in this study. Twenty porcine eyes were soaked in dextran solution for 30 minutes (10 eyes in 2% dextran solution and 10 eyes in 20% dextran solution). CXL treatment was performed in 10 porcine eyes, the other 10 porcine eyes were regarded as a control group. Each eye was fixed on an experimental inflation platform to carry out Corvis measurements at different IOPs. Corneal biomechanical parameters were calculated based on Corvis measurement. Statistical analysis was used to analyze the influence of dextran solution and CXL on corneal biomechanical parameters based on Corvis parameters. Results: Corneal energy absorbed area (Aabsorbed) decreased after being soaked in dextran solution under IOP of 15 mmHg; Corneal elastic modulus (E) decreased after being soaked in 2% dextran solution and increased after being soaked in 20% dextran solution; SP-A1 increased after CXL. Conclusion: Both dextran solution and CXL can change corneal biomechanical properties; SP-A1 may be used as an effective parameter for the evaluation of CXL.

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Investigation into the Elastic Properties of ex vivo Porcine Corneas Subjected to Inflation Test after Cross-Linking Treatment

Abstract: The inflation test has been proven to be a valuable tool for the investigation of corneal biomechanics, maintaining both integrity and geometry of corneal tissue.

Cited by 15 publications

References 39 publications

Estimation of the Corneal Young’s ModulusIn VivoBased on a Fluid-Filled Spherical-Shell Model with Scheimpflug Imaging

Estimation of the Corneal Young’s ModulusIn VivoBased on a Fluid-Filled Spherical-Shell Model with Scheimpflug Imaging

Effect of Topical Antiinflammatory Drugs on Mechanical Behavior of Rabbit Cornea

Influence of dextran solution and corneal collagen crosslinking on Corneal Biomechanical Parameters Evaluated by Corvis ST

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