Investigation Into the Relationship Between Sperm Cysteine-Rich Secretory Protein 2 (CRISP2) and Sperm Fertilizing Ability and Fertility of Boars

Gao, Fenglei; Wang, Ping; Wang, Kai; Fan, Yushan; Chen, Yuming; Chen, Yun; Ye, Chao; Mao, Feng; Li, Li; Zhang, Shouquan; Wei, Hengxi

doi:10.3389/fvets.2021.653413

Cited by 5 publications

(3 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, a correlation was found between CRISP2 expression and low sperm progressive motility, abnormal sperm morphology and infertility, suggesting that the lower expression of CRISP2 in these patients could be due to a post-transcriptional regulation process mediated by miR27 b ( Zhou et al, 2015 ). In agreement with the observations in humans, recent studies revealed a positive correlation between CRISP2 expression levels and boar fertility and that sperm CRISP2 has the potential to serve as a fertility biomarker ( Gao et al, 2021 ).…”

Section: Evidence On the Relevance Of Crisp Proteins For Fertilization And Fertility Through The Use Of Non-genetic Approachessupporting

confidence: 84%

Cysteine-Rich Secretory Proteins (CRISP) are Key Players in Mammalian Fertilization and Fertility

Gonzalez

Sulzyk

Muñoz

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Mammalian fertilization is a complex process involving a series of successive sperm-egg interaction steps mediated by different molecules and mechanisms. Studies carried out during the past 30 years, using a group of proteins named CRISP (Cysteine-RIch Secretory Proteins), have significantly contributed to elucidating the molecular mechanisms underlying mammalian gamete interaction. The CRISP family is composed of four members (i.e., CRISP1-4) in mammals, mainly expressed in the male tract, present in spermatozoa and exhibiting Ca2+ channel regulatory abilities. Biochemical, molecular and genetic approaches show that each CRISP protein participates in more than one stage of gamete interaction (i.e., cumulus penetration, sperm-ZP binding, ZP penetration, gamete fusion) by either ligand-receptor interactions or the regulation of several capacitation-associated events (i.e., protein tyrosine phosphorylation, acrosome reaction, hyperactivation, etc.) likely through their ability to regulate different sperm ion channels. Moreover, deletion of different numbers and combination of Crisp genes leading to the generation of single, double, triple and quadruple knockout mice showed that CRISP proteins are essential for male fertility and are involved not only in gamete interaction but also in previous and subsequent steps such as sperm transport within the female tract and early embryo development. Collectively, these observations reveal that CRISP have evolved to perform redundant as well as specialized functions and are organized in functional modules within the family that work through independent pathways and contribute distinctly to fertility success. Redundancy and compensation mechanisms within protein families are particularly important for spermatozoa which are transcriptionally and translationally inactive cells carrying numerous protein families, emphasizing the importance of generating multiple knockout models to unmask the true functional relevance of family proteins. Considering the high sequence and functional homology between rodent and human CRISP proteins, these observations will contribute to a better understanding and diagnosis of human infertility as well as the development of new contraceptive options.

show abstract

Section: Evidence On the Relevance Of Crisp Proteins For Fertilization And Fertility Through The Use Of Non-genetic Approachessupporting

confidence: 84%

Cysteine-Rich Secretory Proteins (CRISP) are Key Players in Mammalian Fertilization and Fertility

Gonzalez

Sulzyk

Muñoz

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…In Figure 4B, another capture of sperm penetrating the ZP showed that CRISP2 stayed condensed in the post-acrosomal region. A recent study has shown that there is no CRISP2 mRNA expression in porcine ovary [49]. In our study, mature oocytes were collected and processed for immunoblotting analysis.…”

Section: Distribution Of Crisp2 Of Sperm Bound To Zpmentioning

confidence: 93%

The fate of porcine sperm CRISP2 from the perinuclear theca before and after in vitro fertilization

Zhang

Bromfield

Helms

et al. 2022

Biology of Reproduction

View full text Add to dashboard Cite

In a previous study we reported that porcine sperm cysteine rich secretory protein 2 (CRISP2) is localized in the post-acrosomal sheath (PAS)-perinuclear theca (PT) as reduction-sensitive oligomers. In the current study, the decondensation and removal of CRISP2 was investigated during in vitro sperm capacitation, both after induction of the acrosome reaction and after in vitro fertilization. Confocal immunofluorescent imaging revealed that additional CRISP2 fluorescence appeared on the apical ridge and on the equatorial segment (EqS) of the sperm head following capacitation, likely due to cholesterol removal. After an ionophore A23187 induced acrosome reaction, CRISP2 immunofluorescence disappeared from the apical ridge and the EqS area partly due to the removal of the acrosomal shroud vesicles but also partly due to its presence in a subdomain of EqS (EqSS). The fate of sperm head CRISP2 was further examined post-fertilization. In vitro matured porcine oocytes were co-incubated with boar sperm cells for 6-8 h and the zygotes were processed for CRISP2 immunofluorescent staining. Notably, decondensation of CRISP2, and thus of the sperm PT, occurred while the sperm nucleus was still fully condensed. CRISP2 was no longer detectable in fertilized oocytes in which sperm nuclear decondensation and paternal pronucleus formation were apparent. This rapid dispersal of CRISP2 in the PT is likely regulated by redox reactions for which its cysteine rich domain is sensitive. Reduction of disulfide bridges within CRISP2 oligomers may be instrumental for PT dispersal and PT elimination.

show abstract

“…Fresh semen of Duroc pig was obtained from the local livestock breed improvement station. IVF was performed according to a previous reference [ 24 ] with slight modifications; 0.8 mL semen was diluted with 6 mL semen diluent solution mTBM (containing 111.3 mmol/L NaCl, 5.0 mmol/L Sodium pyruvate, 3.0 mmol/L KCl, 7.5 mmol/L CaCl 2 , 11.0 mmol/L Glucose, 0.2% BSA, 20.2 mmol/L Tris) and centrifuged with 1200× g for 5 min at room temperature, and 0.5 mL semen was saved and transferred into 5 mL fertilization fluid (PF) containing mTBM and 2 mmol/L Caffeine and centrifuged with 1200× g for 3 min. A volume of 0.3 mL semen was saved and transferred into a 1.5 mL EP tube for IVF.…”

Section: Methodsmentioning

confidence: 99%

RNA-Seq Reveals the Underlying Molecular Mechanism of First Cleavage Time Affecting Porcine Embryo Development

Song

Xiong

et al. 2022

Genes

View full text Add to dashboard Cite

The selection and evaluation of high-quality embryos are the key factors affecting in vitro embryo development and pregnancy outcome. The timing of first embryonic cleavage has been considered a positive indicator of the in vitro developmental potential of embryos, while the underlying molecular mechanism is still not fully understood. In this study, the embryos generated by parthenogenetic activation (PA) or in vitro fertilization (IVF) were monitored and recorded every 2 h and divided into two groups (early cleavage or late cleavage) based on the cleavage rate and blastocyst formation data. RNA sequencing was used to analyze the gene expression pattern of the embryos. We identified 667 and 71 different expression genes (DEGs) in early cleavage and late cleavage porcine PA and IVF embryos, respectively. Further Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that the DEGs are mainly enriched in pathways concerning the proteasome, DNA repair, cell cycle arrest, autophagy, and apoptosis, suggesting that severe endoplasmic reticulum stress (ERS) and DNA damage may be the key factors that led to the low development potential of late cleavage embryos. This study provides a theoretical basis for the following application and offers important information about the understanding of the timely manner of porcine embryo development.

show abstract

Investigation Into the Relationship Between Sperm Cysteine-Rich Secretory Protein 2 (CRISP2) and Sperm Fertilizing Ability and Fertility of Boars

Cited by 5 publications

References 28 publications

Cysteine-Rich Secretory Proteins (CRISP) are Key Players in Mammalian Fertilization and Fertility

Cysteine-Rich Secretory Proteins (CRISP) are Key Players in Mammalian Fertilization and Fertility

The fate of porcine sperm CRISP2 from the perinuclear theca before and after in vitro fertilization

RNA-Seq Reveals the Underlying Molecular Mechanism of First Cleavage Time Affecting Porcine Embryo Development

Contact Info

Product

Resources

About