Investigation into the repeated recovery of coagulase-negative staphylococci from blood taken at the end of cardiopulmonary by-pass

Belkum, Alex van; Kluijtmans, J.A.J.W.; Leeuwen, Willem van; Goessens, W. H. F.; Averst, Ellen ter; Verbrugh, Henri A.

doi:10.1016/0195-6701(95)90207-4

Cited by 11 publications

(13 citation statements)

References 17 publications

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“…According to current recommendations, two techniques were used to type staphylococci at the genotypic level (33,34). First, all available CoNS isolates from air sample, insertion site skin, exit site, hub, blood, and catheter cultures were genotyped by PCR-based random amplification of polymorphic DNA (RAPD).…”

Section: Methodsmentioning

confidence: 99%

“…Earlier CoNS-genotyping studies have shown persistence of a few CoNS strains in various wards of our hospital. In 1993, more than 30% of CoNS from blood cultures taken from a heart-lung machine during cardiac surgery belonged to a single genotype (33). Furthermore, it was shown that certain CoNS strains permeate the hematology department and colonize its personnel.…”

mentioning

confidence: 99%

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Clonal Expansion of Staphylococcus epidermidis Strains Causing Hickman Catheter-Related Infections in a Hemato-Oncologic Department

et al. 1998

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The detailed analysis of 411 strains of coagulase-negative staphylococci (CoNS) obtained from 40 neutropenic hemato-oncologic patients (61 Hickman catheter episodes) on intensive chemotherapy is described. By random amplification of polymorphic DNA (RAPD) analysis, a total of 88 different genotypes were detected: 51 in air samples and 30 in skin cultures prior to insertion, 12 in blood cultures after insertion, and only 5 involved in catheter-related infections (CRI). Two RAPD genotypes of Staphylococcus epidermidis predominated, and their prevalence increased during patient hospitalization. At insertion, these clones constituted 11 of 86 (13%) CoNS isolated from air samples and 33 of 75 (44%) CoNS isolated from skin cultures. After insertion, their combined prevalence increased to 33 of 62 (53%) in catheters not associated with CRI and 139 of 188 (74%) in catheters associated with CRI (P ‫؍‬ 0.0041). These two predominant S. epidermidis clones gave rise to a very high incidence of CRI (6.0 per 1,000 catheter days) and a very high catheter removal rate for CRI, 70%, despite prompt treatment with vancomycin. A likely source of S. epidermidis strains involved in CRI appeared to be the skin flora in 75% of cases. The validity of these observations was confirmed by pulsed-field gel electrophoresis (PFGE) of SmaI DNA macrorestriction fragments of blood culture CoNS isolates. Again, two predominant CoNS genotypes were found (combined prevalence, 60%). RAPD and PFGE yielded concordant results in 75% of cases. Retrospectively, the same two predominant CoNS clones were also found among blood culture CoNS isolates from the same hematology department in the period 1991 to 1993 (combined prevalence, 42%) but not in the period 1978 to 1982. These observations underscore the pathogenic potential of clonal CoNS types that have successfully and persistently colonized patients in this hemato-oncology department.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

Clonal Expansion of Staphylococcus epidermidis Strains Causing Hickman Catheter-Related Infections in a Hemato-Oncologic Department

et al. 1998

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“…Different combinations of primers with variations in annealing temperature were investigated to achieve maximum discriminatory power. After initial amplification cycles with an annealing temperature of 25°C or 30°C [9], the annealing temperature was increased to 50°C for another five cycles. With this method, the number of faint bands in the DNA fingerprints was decreased.…”

Section: Resultsmentioning

confidence: 99%

PCR-based DNA fingerprinting of Staphylococcus haemolyticus to investigate nosocomial infections

Noordhoek

Scheltinga

Caesar

et al. 1997

Clinical Microbiology and Infection

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OBJECTIVE: To apply PCR-based DNA fingerprinting in a clinical microbiology laboratory to investigate nosocomial infections with Staphylococcus haemolyticus. METHODS: DNA fingerprints were generated by PCR on 99 S. haemolyticus isolates using different primer combinations based on ERIC, REP or arbitrarily chosen simple repeat sequences. RESULTS: Primer combinations REP1+(GTC)6 and ERIC1+ERIC2 had sufficient discrimatory power and were chosen to analyze the clinical isolates. DNA fingerprint patterns from strains isolated from the patients nursed in the same hospital ward in the period 1991--94 were approximately 90% similar to each other. One staff member, sampled in 1991, carried a strain with a similar fingerprint. CONCLUSIONS: PCR based DNA fingerprinting is a suitable method to perform in a clinical laboratory. An S. haemolyticus strain appeared to be endemic in the hospital ward and had most probably been transmitted from patient to patient. S. haemolyticus may carry glycopeptide resistance and needs attention as a causative agent of nosocomial infections.

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“…[9][10][11][12][13] The criterion of multiple positive cultures predicting bloodstream infection implies that the same species and clone of CoNS will be recovered from these cultures. [16][17][18][19] However, it is not uncommon to find different CoNS species or genotypes in sequential blood cultures. [16][17][18][19][20][21][22] Even repeated isolation of CoNS from blood cultures can represent contamination, particularly those obtained several days apart.…”

Section: Discussionmentioning

confidence: 99%

“…[16][17][18][19] However, it is not uncommon to find different CoNS species or genotypes in sequential blood cultures. [16][17][18][19][20][21][22] Even repeated isolation of CoNS from blood cultures can represent contamination, particularly those obtained several days apart. As shown in a previously reported part of the current study, 15 of the 13 paired CoNS isolates available for assessment, the species and genotypes were identical in each paired CoNS isolates, which denotes true bacteraemia.…”

Section: Discussionmentioning

confidence: 99%

Significance of coagulase-negative staphylococci isolated from a single blood culture from neonates in intensive care

Huang

Wang

Chou

et al. 2006

Annals of Tropical Paediatrics

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Investigation into the repeated recovery of coagulase-negative staphylococci from blood taken at the end of cardiopulmonary by-pass

Cited by 11 publications

References 17 publications

Clonal Expansion of Staphylococcus epidermidis Strains Causing Hickman Catheter-Related Infections in a Hemato-Oncologic Department

Clonal Expansion of Staphylococcus epidermidis Strains Causing Hickman Catheter-Related Infections in a Hemato-Oncologic Department

PCR-based DNA fingerprinting of Staphylococcus haemolyticus to investigate nosocomial infections

Significance of coagulase-negative staphylococci isolated from a single blood culture from neonates in intensive care

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