Investigation of 5-(3-Trifluoromethylbenzylidene)thiazolidine-2,4-dione as a Matrix for Analyses of Biogenic Monoamine Transmitters Using MALDI-MS

Abstract

In order to discover new matrices suitable for the analyses of low molecular-weight compounds using positive-ion mode matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry (MS), 5-(3-trifluoromethylbenzylidene)thiazolidine-2,4-dione (3-CF3-BTD) was synthesized, and its effectiveness was compared with that when commercially available α-cyano-4-hydroxycinnamic acid was used. 3-CF3-BTD was sufficiently sensitive to analyze neurotransmitters, i.e., dopamine, serotonin, histamine, and epinephrine, in amounts of several picomoles. Similar to vacuum MALDI experiments, atmospheric-pressure MALDI-MS measurements using 3-CF3-BTD as a matrix also detected dopamine.

Nanoparticle-based applications by atmospheric pressure matrix assisted desorption/ionization mass spectrometry

“…Further, Kasai Hiroko's team synthesized a new matrix, 5-(3-trifluoromethyl benzylidene) thiazolidine-2,4-dione (3-CF3-BTD) to apply in AP MALDI MS in 2016, which could detect dopamine. 62 Notably, Oliver J. Hale and her teammates conducted preliminary optimization of ESI-like ions employing the negative mode in liquid AP MALDI MS in 2018, and peptide, protein, DNA, and lipid were identified using the optimal method. In this experiment, analysis of lipid and DNA benefited from the complementary lipid species and the stronger DNA intensity of the signal.…”