Machine learning models have been used to precisely forecast emissions from diesel engines, specifically examining the impact of various fuel types (HVO10, HVO 30, HVO40, HVO50) on the accuracy of emission forecasts. The research has revealed that models with different numbers of perceptrons had greater initial error rates, which subsequently reached a stable state after further training. Additionally, the research has revealed that augmenting the proportion of Hydrogenated Vegetable Oil (HVO) resulted in the enhanced precision of emission predictions. The use of visual data representations, such as histograms and scatter plots, yielded significant insights into the model’s versatility across different fuel types. The discovery of these results is vital for enhancing engine performance and fulfilling environmental regulations. This study highlights the capacity of machine learning in monitoring the environment and controlling engines and proposes further investigation into enhancing models and making real-time predictive adjustments. The novelty of the research is based on the determination of the input interface (a sufficient amount of input parameters, including chemical as well as technical), which characterizes the different regimes of the diesel engine. The novelty of the methodology is based on the selection of a suitable ANN type and architecture, which allows us to predict the required parameters for a wide range of input intervals (different types of mixtures consisting of HVO and pure diesel, different loads, different RPMs, etc.).