Investigation of a Salmonellosis Outbreak Caused by Multidrug Resistant Salmonella Typhimurium in China

Xiang, Ying; Li, Fuxiang; Dong, Ning; Tian, Shuo; Zhang, Haoran; Du, Xinying; Zhou, Xuan; Xu, Xuebin; Yang, Hang; Xie, Jing; Yang, Chaojie; Liu, Hongbo; Qiu, Shaofu; Song, Hongbin; Sun, Yansong

doi:10.3389/fmicb.2020.00801

Cited by 49 publications

(32 citation statements)

References 45 publications

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“…Scopoletin, gallic acid, and piperic acid exhibited antibacterial activity of both Gram (-) and Gram (+). For example, scopoletin belongs to coumarin resistant Gram (-) (Salmonella typhimurium (MIC of 250 μg/mL [32], Pseudomonas aeruginosa ATCC 27853, and Pseudomonas DMSC 37166 (MIC of 0.66 μg/mL [33], Prevotella intermadia, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans (MIC from 0.25 μg/mL to 1.0 μg/mL and 0.5 μg/mL to 1.0 μg/mL, respectively [34] and Gram (+) (Actinomyces naeslundii, Actinomyces israelii, and Streptococcus mutans) [34]. Scopoletin caused the change of the bacterial cell wall similar to antibiotic β-lactam basing on the inhibition of the cell wall formation and the deformation of the bacterial morphology [33].…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Screening and Evaluation of Antioxidant Properties and Antimicrobial Activity against Xanthomonas axonopodis of Euphorbia tirucalli Extracts in Binh Thuan Province, Vietnam

Cường

Thinh

et al. 2021

Molecules

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Euphorbia tirucalli is a medicine plant possessing many bioactive properties. This paper focused on phytochemical screening (alkaloid, flavonoid, saponin, tannin, and anthraquinone), quantification of polyphenol and flavonoids, and activating evaluation of antioxidants and antimicrobial properties against Xanthomonas axonopodis of different extracts from Euphorbia tirucalli grown in Binh Thuan, Vietnam. The best activity fraction was used for purification and determining bioactive ingredients. The results showed that the phytochemical study revealed the presence of alkaloids, flavonoids, tannins, and terpenoids in the ethyl acetate fraction. Saponin and anthraquinone did not present in all extracts. The content of polyphenol and flavonoid of Euphorbia tirucalli stem was in the range of 16.65–106.32 mg EqAG/g and 97.97–450.83 μg QE/g. The ethyl acetate fraction showed higher amounts of polyphenol and flavonoids and antimicrobial activity against X. axonopodis than other fractions. The antioxidant (SC50) activity of Euphorbia tirucalli stem was in the range of 12.91 ± 0.70 and 528.33 ± 25.15 μg/mL. At concentrations of 5.0 and 7.5 mg/mL, the diameter of inhibition of the ethyl acetate fraction was 14.33 ± 0.76 mm and 17.87 ± 0.57 mm, respectively. The MIC (minimum inhibitory concentration) was 0.156 mg/mL. Scopoletin, gallic acid, and piperic acid got MICs corresponding to 78, 312, and 312 μg/mL, respectively. Scopoletin, gallic acid, and piperic acid were found in the ethyl acetate fraction of Euphorbia tirucalli and exhibited the treatment of citrus bacteria canker and plant diseases.

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Section: Discussionmentioning

confidence: 99%

Phytochemical Screening and Evaluation of Antioxidant Properties and Antimicrobial Activity against Xanthomonas axonopodis of Euphorbia tirucalli Extracts in Binh Thuan Province, Vietnam

Cường

Thinh

et al. 2021

Molecules

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“…Moreover, S. Typhimurium is the second most prevalent serotype in China (Lu et al, 2011). Some studies had found that S. Typhimurium was exhibiting a multi-drug resistance pattern and carried a large number of virulence genes (Reis et al, 2018;Xiang et al, 2020). With the emergence of antimicrobialresistant Salmonella, the epidemic multidrug-resistant (MDR) S. Typhimurium strain definitive phage type 104 (DT104), first observed during the 1990s (Mølbak et al, 1999), became increasingly prevalent.…”

Section: Introductionmentioning

confidence: 99%

Plasmid-borne colistin resistance gene mcr-1 in a multidrug resistant Salmonella enterica serovar Typhimurium isolate from an infant with acute diarrhea in China

Zhang

Chen³

et al. 2021

International Journal of Infectious Diseases

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Background: Antimicrobial resistance of Salmonella enterica is a major global concern. Recent findings suggest that colistin as a last resort treatment for multidrug-resistant gram-negative bacteria is seriously threatened by the report of plasmid-mediated colistin resistance gene mcr-1 in China. Methods: A total of 827 S. Typhimurium isolates were recovered from 4 cities of China, including Henan, Shanghai, Zhejiang, and Hubei provinces. Subsequently, mcr-1 presence was identified by PCR screening. Antimicrobial susceptibility testing was performed by broth microdilution using a 96-well microtiter plate. Plasmid conjugation transfer experiments were conducted using Escherichia coli J53 as the recipient.Results: Only one mcr-1 positive strain from the stool sample of an infant with acute diarrhea was isolated. Apart from colistin, the mcr-1-positive isolate showed co-resistance to the third-generation cephalosporins, ampicillin, nalidixic acid, tetracycline, chloramphenicol, sulfisoxazole, gentamicin, and cefotaxime revealing a multidrug-resistant phenotype. This strain harbored mcr-1 on a 227 kb IncHI2 plasmid, termed pJZ26, which could be transferred to E. coli J53. In addition to mcr-1, pJZ26 coharbored other resistance genes, including aph(4)-Ia,aac(3)-IVa, fosA, floR, sul2, and bla CTX-M-14 . Compared with p2474-MCR1 and pHYEC7-IncHI2, pJZ26 contains an additional 4.6 kb fragment harboring the resistance gene tet(A) and its regulator tetR located on TnAs1 transposable element, which could mediate resistance to tetracycline. Conclusions: These findings highlight that the fact the mcr-1-harboring plasmid pJZ26 has a high potential to disseminate the mcr-1 gene and further challenge the clinical treatment.

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“…However, recent reports from India suggest [16] that antibiotic alternatives (dietary probiotic supplements) known to reduce MDR S. Typhimurium colonization in pigs can harbor resistance to many antibiotics, including ceftazidime, when misused. Moreover, a recent outbreak of Salmonellosis in China involving MDR S. Typhimurium [17] further highlighted the need for training and rigorous monitoring of these programs. Gene analysis of the serovar showed it contained several resistant genes, including bla OXA-1 , bla TEM-1 , and β-lactamase.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in the Detection of Antibiotic and Multi-Drug Resistant Salmonella: An Update

Hulme

2021

IJMS

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Antibiotic and multi-drug resistant (MDR) Salmonella poses a significant threat to public health due to its ability to colonize animals (cold and warm-blooded) and contaminate freshwater supplies. Monitoring antibiotic resistant Salmonella is traditionally costly, involving the application of phenotypic and genotypic tests over several days. However, with the introduction of cheaper semi-automated devices in the last decade, strain detection and identification times have significantly fallen. This, in turn, has led to efficiently regulated food production systems and further reductions in food safety hazards. This review highlights current and emerging technologies used in the detection of antibiotic resistant and MDR Salmonella.

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Investigation of a Salmonellosis Outbreak Caused by Multidrug Resistant Salmonella Typhimurium in China

Cited by 49 publications

References 45 publications

Phytochemical Screening and Evaluation of Antioxidant Properties and Antimicrobial Activity against Xanthomonas axonopodis of Euphorbia tirucalli Extracts in Binh Thuan Province, Vietnam

Phytochemical Screening and Evaluation of Antioxidant Properties and Antimicrobial Activity against Xanthomonas axonopodis of Euphorbia tirucalli Extracts in Binh Thuan Province, Vietnam

Plasmid-borne colistin resistance gene mcr-1 in a multidrug resistant Salmonella enterica serovar Typhimurium isolate from an infant with acute diarrhea in China

Recent Advances in the Detection of Antibiotic and Multi-Drug Resistant Salmonella: An Update

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