Investigation of ace genome insertion/deletion correlation with immunohistochemical profile in pituitary adenomas

Abstract

AIm:The deletion polymorphism of the angiotensin-converting enzyme (ACE) genome causes neoplastic development in several organs by increasing the angiotensin 2 (A2) formation. In this study, we aimed to identify the ACE genome insertion/deletion polymorphism in pituitary adenomas and to compare it with the control group. mAterIAl and methOds: Patients operated for pituitary adenomas were included in the study. Genomic DNA was extracted from tumoral tissues and peripheral blood samples of the patients by using the Miller method. Primary sequence was selected via targeting the polymorphic region of intron 16 of ACE genome 17q23. DNA samples were multiplied by PCR using HACE3s and HACE3as primers.results: Twenty-one operated cases were studied. In the study group; 44 % of the patients were identified as D/D, 33% of them as I/D and 23% of them as I/I. In 60%, D allele was identified. According to immunohistochemical investigation, we found that 100% of the patients with Cushing adenoma were D/D alleles. COnClusIOn:Presence of high rate of ACE genome deletion in patients with pituitary adenoma and grade 3-4 patients suggest that ACE genome polymorphism can be a risk factor for the development of pituitary adenomas. KeywOrds: Angiotensin-converting enzyme, Pituitary adenomas, Gene ÖZAmAÇ: Anjiotensin Converting Enzim (ACE) genindeki bir delesyon Anjiotensin 2 de bir artış sağlayarak çeşitli organlarda neoplastik gelişmelere neden olur. Biz bu çalışmada hipofiz adenomlu hastalarda ACE geninde insersiyon /delesyon polimorfizmini belirleyerek kontrol grubuyla karşılaştırmayı amaçladık. sOnuÇ: Kontrol grubuyla karşılaştırıldığında özellikle Grade 3-4 olan hipofiz adenomlu hastalarda yüksek oranda ACE geninde delesyon varlığı hipofiz adenomu gelişiminde ACE geninin bir risk faktörü olabileceğini düşündürmektedir.

Contribution of Endothelial Dysfunction to Cancer Susceptibility and Progression: A Comprehensive Narrative Review on the Genetic Risk Component

“…Additionally, the ID genotype is also suggested to exert a protective role against BC [114]. The D allele presence is associated with an increased risk of uterine leiomyoma [115]; gall bladder carcinoma (GBC) [116], and glioma [117], while the homozygous D genotype is associated with increased susceptibility to glioma development and low overall survival [118,119]; renal cell carcinoma (RCC) [120]; BLCA [121]; basal cell carcinoma (BCC) [122][123][124]; poor leukaemia survival rates [125]; lymph nodes metastasis in laryngeal cancer (LaC) [126]; pituitary adenomas development and progression [127]; and EMCA [128]. Regarding cancer patients' prognosis, while a direct impact is not described, the ACE ID genotype was associated with higher haemoglobin levels and overall lower fat mass and muscle strength in patients at advanced stages compared to the II genotype [129].…”