CHAPTER V EFFECTS OF IONIZATION ON INTERMOLECULAR INTERACTION…………………………………………….…..110 Introduction…………………………………………..….………………..110 Materials and Methods………………………………….………………...111 Materials………………………………………………...………….111 Methods………………………………………….………………….111 Preparation of PAMAM-daptomycin complex.…………….111 Fluorescence spectroscopy……………..…………………...112 Binding isotherm constructions…………………………….112 Estimation of the binding parameters………………………113 Results…………………………………………………………………….114 Fluorescence Properties of PAMAM-daptomycin Complex……….114 Effects of pH and Dendrimer Size on the Binding Characteristics of Daptomycin Dendrimer Complex……...………..119 Binding Parameters…………………………………………...….…122 Discussion……………………………………………………………...…123 Conclusions………………………………………………………….……125 vi APPENDIX A. DAPTOMYCIN BJERRUM PLOT………….………...129 APPENDIX B.