Investigation of air-DBD effects on biological liquids for in vitro studies on eukaryotic cells

Trizio, Ilaria; Sardella, Eloisa; Francioso, Edda; Dilecce, Giorgio; Rizzi, Vito; Cosma, Pinalysa; Schmidt, Michael; Hänsch, Mareike; Woedtke, Thomas von; Favia, Pietro; Gristina, Roberto

doi:10.1016/j.cpme.2015.09.003

Cited by 13 publications

(20 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For comprehensive summaries of the broad variety of discharges in combination with liquids, please refer to [1,14,15]. Regarding the treatment of water, changes of its properties have been examined by many researchers [6][7][8][9][16][17][18][19]. In all cases of discharges operated in contact with or in close vicinity to water, acidification (except for tap water) and nitration was observed.…”

Section: Introductionmentioning

confidence: 99%

Plasma-Activation of Larger Liquid Volumes by an Inductively-Limited Discharge for Antimicrobial Purposes

et al. 2019

Self Cite

View full text Add to dashboard Cite

A new configuration of a discharge chamber and power source for the treatment of up to 1 L of liquid is presented. A leakage transformer, energizing two metal electrodes positioned above the liquid, limits the discharge current inductively by utilizing the weak magnetic coupling between the primary and secondary coils. No additional means to avoid arcing (electric short-circuiting), e.g., dielectric barriers or resistors, are needed. By using this technique, exceeding the breakdown voltage leads to the formation of transient spark discharges, producing non-thermal plasma (NTP). These discharges effected significant changes in the properties of the treated liquids (distilled water, physiological saline solution, and tap water). Considerable concentrations of nitrite and nitrate were detected after the plasma treatment. Furthermore, all tested liquids gained strong antibacterial efficacy which was shown by inactivating suspended Escherichia coli and Staphylococcus aureus. Plasma-treated tap water had the strongest effect, which is shown for the first time. Additionally, the pH-value of tap water did not decrease during the plasma treatment, and its conductivity increased less than for the other tested liquids.

show abstract

Section: Introductionmentioning

confidence: 99%

Plasma-Activation of Larger Liquid Volumes by an Inductively-Limited Discharge for Antimicrobial Purposes

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…3D models instead, are a compromise between the 2D reductionist approach and the complexity of in vivo models . It is known in literature and we have also reported that the effect of PAM on cell behavior is both dose‐dependent and cell‐type specific . In particular, the loss of viability and of the typical morphology was mainly observed on SAOS‐2 cells after exposure to PAM.…”

Section: Resultsmentioning

confidence: 87%

“…Figure A reports fluorescence measurements of H 2 O 2 performed with DCFH 2 ‐DA soon after (i.e., the shortest time needed form the plasma treatment to the fluorescence analysis) the plasma treatment. Harsh PAM shows a slight increase of the fluorescence intensity with respect the untreated sample; the addition of Catalase quenched the fluorescence by transforming H 2 O 2 in water according to equation , evidencing that traces of H 2 O 2 were generated in the medium . It must be underlined that the DCFH 2 ‐DA is reported in the literature as a probe also suitable for the peroxynitrite (ONOO − ) detection .…”

Section: Resultsmentioning

confidence: 95%

“…In particular, the loss of viability and of the typical morphology was mainly observed on SAOS‐2 cells after exposure to PAM. On the other hand, however, we have also reported an enhancement of cell viability of BMSC cells exposed to PAM activated at low energy doses, that stimulated us to investigate the possibility of using PAM to activate certain cell activities (adhesion, growth, proliferation) on 3D scaffolds, that are auspicated in Tissue Engineering. It has to be stressed, for in vitro cell culture experiments, that results from primary cells are highly dependent on their source.…”

Section: Resultsmentioning

confidence: 99%

“…Figure S1 reports the electrical scheme of the equipment. The complete description and the scheme of the DBD is reported in ref . Two different plasma conditions were compared, defined as Mild and Harsh for simplicity, characterized, respectively, by 30 and 60 s of treatment time (see Table S1).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Plasma generated RONS in cell culture medium for in vitro studies of eukaryotic cells on Tissue Engineering scaffolds

Trizio

Rizzi

Gristina

et al. 2017

Plasma Process Polym

Self Cite

View full text Add to dashboard Cite

Cold plasmas are continuously developed for biomaterials engineering as well as for therapeutic treatments of cells and tissues. For this last application plasma activated media (PAM) and reactive oxygen and nitrogen species (RONS) gained attention as key players. Here, the use of cold atmospheric pressure (AP) plasma is described, for generating RONS in Dulbecco's Modified Eagles Medium (DMEM); superoxide anion (normalO2−), hydrogen peroxide (H2O2), nitrates (NO3−), and nitrites (NO2−) were detected. PAM was applied to Bone Marrow Stem Cells (BMSC) and SAOS‐2 osteoblasts. Both native and plasma‐modified polymeric scaffolds were used as three dimensional (3D) supports for cell cultures. The cell activity was found dependent on both PAM and cell type. BMSCs grown on plasma‐coated scaffolds tolerated better PAM with respect to those on native scaffolds.

show abstract

Validation of colorimetric assays for hydrogen peroxide, nitrate and nitrite ions in complex plasma‐treated water solutions

Veronico

Favia

Fracassi

et al. 2021

Plasma Processes & Polymers

Self Cite

View full text Add to dashboard Cite

Liquids treated with cold plasma emerged as ‘redox drugs’ in biomedicine, as sources of reactive oxygen and nitrogen species targeting cellular functions, including wound healing and cancer progression. The use of cell culture media as starting liquid, however, challenges the identification of plasma‐generated chemistry, limited by the presence of many reactive species and organic compounds. Available detection methods need, therefore, to be confirmed in these liquids to avoid inaccurate results. In this research, robustness, linearity, accuracy and specificity of three colorimetric assays are investigated to detect H2O2, NO2− and NO3−, predominant plasma‐induced products. The results clearly highlight the presence of some factors affecting the detection in cell culture media like high concentrations of chlorides found interfering with the detection of NO3− in the medium.

show abstract

Investigation of air-DBD effects on biological liquids for in vitro studies on eukaryotic cells

Cited by 13 publications

References 44 publications

Plasma-Activation of Larger Liquid Volumes by an Inductively-Limited Discharge for Antimicrobial Purposes

Plasma-Activation of Larger Liquid Volumes by an Inductively-Limited Discharge for Antimicrobial Purposes

Plasma generated RONS in cell culture medium for in vitro studies of eukaryotic cells on Tissue Engineering scaffolds

Validation of colorimetric assays for hydrogen peroxide, nitrate and nitrite ions in complex plasma‐treated water solutions

Contact Info

Product

Resources

About