Investigation of amino acid δ13C signatures in bone collagen to reconstruct human palaeodiets using liquid chromatography–isotope ratio mass spectrometry

Choy, Kyungcheol; Smith, Colin; Fuller, Benjamin T.; Richards, Michael P.

doi:10.1016/j.gca.2010.07.025

Cited by 55 publications

(71 citation statements)

References 62 publications

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“…We suggest the use of carbon isotope analysis of bulk tissues or amino acids to address this problem. Advances in this field are expected from recent developments and improvements in methodologies for CSIA of amino acids using d 13 C (Corr et al 2007a, b;Smith et al 2009;Chikaraishi and Ohkouchi 2010;Choy et al 2010). CSIA of amino acids, using both d 13 C and d 15 N values, would facilitate the study of ecological food webs where C3, C4, and aquatic plants are present.…”

Section: Discussionmentioning

confidence: 99%

¹⁵N/¹⁴N ratios of amino acids as a tool for studying terrestrial food webs: a case study of terrestrial insects (bees, wasps, and hornets)

Chikaraishi

Ogawa

Doi

et al. 2011

Ecological Research

115

148

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Compound-specific stable isotope analysis (CSIA) of amino acids is a new method that enables estimates of trophic position for consumers in food webs. We examined the nitrogen isotopic composition (d 15 N) of amino acids of Japanese social insects (three bee, three wasp, and four hornet species) to evaluate the potential of CSIA of amino acids in studies of terrestrial food webs. For wasps, we also examined samples at different growth stages (ranging from egg to adult) to assess the effect of metamorphosis on CSIA estimates of trophic position. The d 15 N values of bulk tissues for Japanese social insects are only weakly correlated with the biologically expected trophic positions. In contrast, the trophic positions estimated from the d 15 N values of amino acids (yielding values of between 2.0 and 2.3 for bees, between 2.8 and 3.3 for wasps, and between 3.5 and 4.1 for hornets) are consistent with the biologically expected trophic positions for these insects (i.e., 2.0 for bees, 3.0 for wasps, and 3.0-4.0 for hornets). Although large variability is observed among the d 15 N values of individual amino acids (e.g., ranging from 3.0 to 14.9& for phenylalanine), no significant change is observed in the trophic position during wasp metamorphosis. Thus, the CSIA of amino acids is a powerful tool for investigating not only aquatic food webs but also terrestrial food webs with predatory insects.

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Section: Discussionmentioning

confidence: 99%

¹⁵N/¹⁴N ratios of amino acids as a tool for studying terrestrial food webs: a case study of terrestrial insects (bees, wasps, and hornets)

Chikaraishi

Ogawa

Doi

et al. 2011

Ecological Research

115

148

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“…[8][9][10][11][12][13][14] AA stable isotope analysis also has the potential to elucidate observed differences in the isotopic composition of body proteins, to understand how food components are used in the production of body tissues, and to derive dietary information from diagenetically modified proteins. [10,11,15] Given the promise of AA stable isotope analysis, interpretive frameworks are being developed to synthesise a growing body of compoundspecific isotope data.…”

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confidence: 99%

“…[10,11,15] Given the promise of AA stable isotope analysis, interpretive frameworks are being developed to synthesise a growing body of compoundspecific isotope data. [8,9,16,17] The gas chromatographycombustion-isotope ratio mass spectrometry (GC/C/IRMS) analysis of skeletal collagen AAs, for instance, has revealed a proxy for the detection of marine protein consumption, where differences in the d 13 C values between the AAs glycine (Gly) and phenylalanine (Phe) are substantially greater for consumers of marine foods than for those consuming primarily terrestrial-based resources (Δ 13 C Glycine-Phenylalanine ). [9] This finding has stimulated the investigation of other proxies to identify the same phenomenon.…”

mentioning

confidence: 99%

“…[9] This finding has stimulated the investigation of other proxies to identify the same phenomenon. [8,16] Despite these advances, it is clear that the interpretive frameworks ultimately depend on a firm understanding of the capabilities of the analytical technologies that are used to produce compound-specific data: an area in which surprisingly little comparative work has been devoted, but which this paper aims to address.…”

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confidence: 99%

“…13 C values, [8,12,14,37,38] and has been applied in a number of areas within life sciences research. [39] Derivatisation is not required for LC/IRMS analysis as the AAs are separated by aqueous HPLC and oxidised on-line, which reduces the analytical error in d…”

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confidence: 99%

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Comparison of liquid chromatography–isotope ratio mass spectrometry (LC/IRMS) and gas chromatography–combustion–isotope ratio mass spectrometry (GC/C/IRMS) for the determination of collagen amino acid δ¹³C values for palaeodietary and palaeoecological reconstruction

Dunn

Honch

Evershed

2011

Rapid Comm Mass Spectrometry

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Results are presented of a comparison of the amino acid (AA) δ(13)C values obtained by gas chromatography-combustion-isotope ratio mass spectrometry (GC/C/IRMS) and liquid chromatography-isotope ratio mass spectrometry (LC/IRMS). Although the primary focus was the compound-specific stable carbon isotope analysis of bone collagen AAs, because of its growing application for palaeodietary and palaeoecological reconstruction, the results are relevant to any field where AA δ(13)C values are required. We compare LC/IRMS with the most up-to-date GC/C/IRMS method using N-acetyl methyl ester (NACME) AA derivatives. This comparison involves the analysis of standard AAs and hydrolysates of archaeological human bone collagen, which have been previously investigated as N-trifluoroacetyl isopropyl esters (TFA/IP). It was observed that, although GC/C/IRMS analyses required less sample, LC/IRMS permitted the analysis of a wider range of AAs, particularly those not amenable to GC analysis (e.g. arginine). Accordingly, reconstructed bulk δ(13)C values based on LC/IRMS-derived δ(13)C values were closer to the EA/IRMS-derived δ(13)C values than those based on GC/C/IRMS values. The analytical errors for LC/IRMS AA δ(13)C values were lower than GC/C/IRMS determinations. Inconsistencies in the δ(13)C values of the TFA/IP derivatives compared with the NACME- and LC/IRMS-derived δ(13)C values suggest inherent problems with the use of TFA/IP derivatives, resulting from: (i) inefficient sample combustion, and/or (ii) differences in the intra-molecular distribution of δ(13)C values between AAs, which are manifested by incomplete combustion. Close similarities between the NACME AA δ(13)C values and the LC/IRMS-derived δ(13)C values suggest that the TFA/IP derivatives should be abandoned for the natural abundance determinations of AA δ(13)C values.

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Beyond diet reconstruction: Stable isotope applications to human physiology, health, and nutrition

Reitsema¹

2013

Am. J. Hum. Biol.

100

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Investigation of amino acid δ13C signatures in bone collagen to reconstruct human palaeodiets using liquid chromatography–isotope ratio mass spectrometry

Cited by 55 publications

References 62 publications

¹⁵N/¹⁴N ratios of amino acids as a tool for studying terrestrial food webs: a case study of terrestrial insects (bees, wasps, and hornets)

¹⁵N/¹⁴N ratios of amino acids as a tool for studying terrestrial food webs: a case study of terrestrial insects (bees, wasps, and hornets)

Comparison of liquid chromatography–isotope ratio mass spectrometry (LC/IRMS) and gas chromatography–combustion–isotope ratio mass spectrometry (GC/C/IRMS) for the determination of collagen amino acid δ¹³C values for palaeodietary and palaeoecological reconstruction

Beyond diet reconstruction: Stable isotope applications to human physiology, health, and nutrition

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Investigation of amino acid δ13C signatures in bone collagen to reconstruct human palaeodiets using liquid chromatography–isotope ratio mass spectrometry

Cited by 55 publications

References 62 publications

15N/14N ratios of amino acids as a tool for studying terrestrial food webs: a case study of terrestrial insects (bees, wasps, and hornets)

15N/14N ratios of amino acids as a tool for studying terrestrial food webs: a case study of terrestrial insects (bees, wasps, and hornets)

Comparison of liquid chromatography–isotope ratio mass spectrometry (LC/IRMS) and gas chromatography–combustion–isotope ratio mass spectrometry (GC/C/IRMS) for the determination of collagen amino acid δ13C values for palaeodietary and palaeoecological reconstruction

Beyond diet reconstruction: Stable isotope applications to human physiology, health, and nutrition

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¹⁵N/¹⁴N ratios of amino acids as a tool for studying terrestrial food webs: a case study of terrestrial insects (bees, wasps, and hornets)

¹⁵N/¹⁴N ratios of amino acids as a tool for studying terrestrial food webs: a case study of terrestrial insects (bees, wasps, and hornets)

Comparison of liquid chromatography–isotope ratio mass spectrometry (LC/IRMS) and gas chromatography–combustion–isotope ratio mass spectrometry (GC/C/IRMS) for the determination of collagen amino acid δ¹³C values for palaeodietary and palaeoecological reconstruction