Investigation of an integrated approach for bio‐crude recovery and enzymatic hydrolysis of microalgae cellulose for glucose production

Aguirre, Ana‐Maria; Bassi, Amarjeet

doi:10.1002/cjce.22388

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…Historically, CAP has employed a dilute Brønsted acid pretreatment, − which proved generally robust for high-carbohydrate and high-lipid algae. However, we hypothesized that alternative pretreatments, such as alkaline hydrolysis, flash hydrolysis, ,− and enzymatic hydrolysis, − may be more robust for high protein and/or variable composition biomass. Similarly, in developing alternative pretreatment approaches, we identified potential opportunities for process intensification.…”

Section: Introductionmentioning

confidence: 99%

De-risking Pretreatment of Microalgae To Produce Fuels and Chemical Co-products

Kruger,

Schutter,

Knoshaug

et al. 2024

Energy Fuels

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Conversion of microalgae to renewable fuels and chemical co-products by pretreating and fractionation holds promise as an algal biorefinery concept, but a better understanding of the pretreatment performance as a function of algae strain and composition is necessary to de-risk algae conversion operations. Similarly, there are few examples of algae pretreatment at scales larger than the bench scale. This work aims to de-risk algal biorefinery operations by evaluating the pretreatment performance across nine different microalgae samples and five different pretreatment methods at small (5 mL) scale and further de-risk the operation by scaling pretreatment for one species to the 80 L scale. The pretreatment performance was evaluated by solubilization of feedstock carbon and nitrogen [as total organic carbon (TOC) and total nitrogen (TN)] into the aqueous hydrolysate and extractability of lipids [as fatty acid methyl esters (FAMEs)] from the pretreated solids. A range of responses was noted among the algae samples across pretreatments, with the current dilute Brønsted acid pretreatment using H 2 SO 4 being the most consistent and robust. This pretreatment produced TOC yields to the hydrolysate ranging from 27.7 to 51.1%, TN yields ranging from 12.3 to 76.2%, and FAME yields ranging from 57.9 to 89.9%. In contrast, the other explored pretreatments (other dilute acid pretreatments, dilute alkali pretreatment with NaOH, enzymatic pretreatment, and flash hydrolysis) produced lower or more variable yields across the three metrics. In light of the greater consistency across samples for dilute acid pretreatment, this method was scaled to 80 L to demonstrate scalability with microalgae feedstocks.

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Section: Introductionmentioning

confidence: 99%

De-risking Pretreatment of Microalgae To Produce Fuels and Chemical Co-products

Kruger,

Schutter,

Knoshaug

et al. 2024

Energy Fuels

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“…Various physical or chemical methods have been employed to degrade microalgae cell walls, including homogenisation, sonication, microwave treatment, heat treatment, and acid or alkaline lysis, all with variable efficiencies [19,20]. Enzymatic degradation (either alone or in combination with physicochemical treatments) and saccharification has also been tested through use of various commercial enzymes, including cellulase, chitinase, βglucuronidase, lysozyme, and pectinase [15,[20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Isolation of fungal strains for biodegradation and saccharification of microalgal biomass

Monjed

Robson

Pittman

2020

Biomass and Bioenergy

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Products from microalgae have multiple commercial applications including for nutrition, health and fuel uses, but the breakage and release of products from biomass remains a challenge, particularly for microalgal strains with thick cell walls. Fungal strains were isolated due to their ability to degrade microalgal biomass of Parachlorella hussii, Hindakia tetrachotoma and Jaagichlorella luteoviridis that was buried in soil at 25°C for 8 weeks. Fungal isolates were identified by sequencing, with the three most ubiquitous strains identified as Fusarium solani, Doratomyces nanus and Actinomucor elegans.Crude enzyme extracts from these strains were used to quantify the saccharification of intact or lipid-extracted Chlorella vulgaris biomass. The D. nanus extract gave the highest saccharification efficiency of 57% from the intact biomass and 76% from the lipid-extracted biomass, without need for any pre-treatment. Outcomes of this study can improve the design of microbial enzymes to efficiently degrade microalgal biomass for various industrial applications.

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Mechanism study on the regulation of metabolite flux for producing promising bioactive substances in microalgae Desmodesmus sp.YT through salinity stress

Chen

Wong

et al. 2022

Algal Research

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Investigation of an integrated approach for bio‐crude recovery and enzymatic hydrolysis of microalgae cellulose for glucose production

Cited by 3 publications

References 30 publications

De-risking Pretreatment of Microalgae To Produce Fuels and Chemical Co-products

De-risking Pretreatment of Microalgae To Produce Fuels and Chemical Co-products

Isolation of fungal strains for biodegradation and saccharification of microalgal biomass

Mechanism study on the regulation of metabolite flux for producing promising bioactive substances in microalgae Desmodesmus sp.YT through salinity stress

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