Investigation of an outbreak of brucellosis in a mixed dairy farm and evaluation of a test and slaughter strategy to release the herd out of the quarantine

El-Diasty, Mohamed; Salah, Khaled; Tawab, Ashraf A. Abd El; Soliman, Enas A.

doi:10.51585/gjvr.2022.1.0028

Cited by 9 publications

(10 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The control strategies for livestock brucellosis are different from region to region depending on available disease prevalence in each region. It has been well documented that test and slaughter strategy (El‐Diasty et al., 2022) and vaccination are among the most effective methods used in controlling and preventing animal brucellosis in endemic regions (El‐Diasty et al., 2022; Hou et al., 2019). Four vaccines, S2, RB51, S19, and Rev1, are available to control brucellosis due to B. abortus infections in bovids and B. melitensis in small ruminants (Hou et al., 2019; Lalsiamthara & Lee, 2017; Zhu et al., 2016).…”

Section: Resultsmentioning

confidence: 99%

“…There are few valid data on the actual prevalence of brucellosis in ruminant livestock of the Middle Eastern countries available (Benkirane, 2006). Different studies showed that older animals and mixed farming systems of small ruminants were significantly associated with a higher prevalence of brucellosis (Abdelbaset et al., 2018; Dadar et al., 2021; El‐Diasty et al., 2022; Samaha et al., 2009). Furthermore, few studies showed that a higher risk of brucellosis is associated with exposure to abortive animals and recently calved animals (Hotez et al., 2012; Musallam et al., 2016; Pappas & Memish, 2007).…”

Section: Resultsmentioning

confidence: 99%

Section: Brucellosis and Brucella Control In The Mena Regionmentioning

confidence: 99%

See 2 more Smart Citations

The perspective of antibiotic therapeutic challenges of brucellosis in the Middle East and North African countries: Current situation and therapeutic management

Wareth

Dadar

Ali

et al. 2022

Transbounding Emerging Dis

View full text Add to dashboard Cite

Brucellosis is among the most prevalent zoonotic infections in Middle Eastern and North African (MENA) countries, critically impacting human and animal health. A comprehensive review of studies on antibiotic susceptibility and therapeutic regimes for brucellosis in ruminants and humans in the MENA region was conducted to evaluate the current therapeutic management in this region. Different scientific databases were searched for peer‐reviewed original English articles published from January 1989 to February 2021. Reports from research organizations and health authorities have been taken into consideration. Brucella melitensis and Brucella abortus have been reported from the majority of MENA countries, suggesting a massive prevalence particularly of B. melitensis across these countries. Several sporadic cases of brucellosis relapse, therapeutic failure, and antibiotic resistance of animal and human isolates have been reported from the MENA region. However, several studies proved that brucellae are still in‐vitro susceptible to the majority of antibiotic compounds and combinations in current recommended World Health Organization (WHO) treatment regimens, for example, levofloxacin, tetracyclines, doxycycline, streptomycin, ciprofloxacin, chloramphenicol, gentamicin, tigecycline, and trimethoprim/sulfamethoxazole. The current review presents an overview on resistance development of brucellae and highlights the current knowledge on effective antibiotics regimens for treating human brucellosis.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Brucellosis and Brucella Control In The Mena Regionmentioning

confidence: 99%

See 1 more Smart Citation

The perspective of antibiotic therapeutic challenges of brucellosis in the Middle East and North African countries: Current situation and therapeutic management

Wareth

Dadar

Ali

et al. 2022

Transbounding Emerging Dis

View full text Add to dashboard Cite

show abstract

“…Egypt's brucellosis control programmes have used two approaches: vaccination to susceptible animals and slaughter of the positive serologic cases. The test-and-slaughter strategy, as well as mass vaccination of animals, continue the most applied methods for controlling brucellosis in endemic countries (Vatankhah et al, 2019;El-Diasty et al, 2022). As a result, ongoing research is critical to preventing transmission and identifying new reservoir areas (Zameer Durrani et al, 2020).…”

Section: Internal Medicine and Infectious Diseasementioning

confidence: 99%

Cross-sectional study and Building a Geographical Information System for Brucellosis in Monufiya

Mousa

Gaafar²,

Zaghawa³

et al. 2022

Journal of Current Veterinary Research

View full text Add to dashboard Cite

Background/ Aim: Brucellosis is a communicable disease with highly significance in human and veterinary practices as well as responsible forsubstantial economic losses in cattle. This study aimed to detect the incidence of brucellosis for three successive years from 2016-2018 in Monufiya governorate using Rose Bengal test. In addition to apply the Geographic Information System (GIS) 10.1 to investigate the patterns of the disease and to identify high-risk areas of the disease. Methods: A total of 75,697 animals (43,274 cattle, 19,974 buffaloes, 8,684 sheep, and 3,765 goats) were tested by RBPT and the obtained data were subjected to the Geographic Information System (GIS) 10.1 to investigate the patterns of the disease over the period from 2016-2018 and to identify high-risk areas of the disease. Results: The reported results indicated that the highest incidence rate of brucellosis in Ashmoun center 3% other than in other cities in cattle and buffaloes. In the other side, the incidence rates in both sheep and goats varied widely between cities and even in the same city within the three years. Conclusion: The animal's movement represent a major source of infection dissemination in Monufiya governorate. Strict measures and preventive strategies must be regulating to prevent the dissemination of brucellosis in Monufiya, especially in moveable flocks.

show abstract

“…Serological diagnosis of Brucella on serum samples was performed through Rose Bengal Test (RBT) and Buffered Acidified Plate Antigen Test (BAPAT), while milk ring test (MRT) in milk is still our first choice to catch the positive cases. MRT, RBT, and BAPAT are the most prevalent screening serological tests but eradication programs can't depend on MRT alone because little titer of antibodies in milk and factors of fat collecting lead to decrease its sensitivity [13,14]. MRT not only be used in bulk milk samples analysis but used also to test the individual milk samples.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Molecular Differentiation of Brucella Species ‎in Buffaloe Raw Milk and Karish Cheese in Egypt

El-Diasty

Shahin²,

Elsherbini³

et al. 2022

Zagazig Veterinary Journal

Self Cite

View full text Add to dashboard Cite

Ninety buffalo's milk samples included 20 bulk tank milk samples from small dairy farms and 70 from retail milk at different localities in Dakahlia Governorate. Forty-eight individual milk specimens were obtained from animals positive for Rose Bengal Test (RBT), buffered acidified plate antigen test (BAPAT) together. Milk samples were tested with a milk ring test (MRT), followed by isolation and identification of the prevalent serotypes. At the same time, conventional PCR was applied. One hundred and sixty seven buffalo's serum samples were collected from 12 smallholder's herds and their bulk tanks gave positive to MRT were confirmed by BAPAT and RBT. A total of 50 karish cheese were collected from street vendors and supermarkets located in Mansoura city, Dakahlia Governorate, Egypt. Conventional PCR was also used to detect Brucella DNA at the species level on the collected cheese samples. The results showed that the prevalence of brucellosis in small dairy buffalo farms was 60% by MRT and 28.7% though using RBT and BAPAT. The prevalence of Brucella in buffalo's retail milk was 20% in each of Bilqase, Sherbin, and Talkha, 30% in each of Aga and Nabroh, 10% in Sinblaween. However no positive results was revealed in Mansoura. The isolation rate of Brucella was 31.25% in individual positive samples and 7.69% in retail positive milk samples. While using conventional PCR, the percentage reached 62.5% in individual positive samples and 69.23% in retail positive milk samples. All Brucella isolates (n = 16) were biochemically identified into three Brucella abortus biovar1 (18.75%) and 13 Brucella melitensis biovar 3 (81.25%). The prevalence of Brucella in examined Karish cheese was 20%. All of the tested isolates were verified using conventional PCR. Raw milk and unpasteurized soft cheese have public health risks for humans and are possible sources of transmitting Brucella and must be under regular and mandatory food control measures.

show abstract

Investigation of an outbreak of brucellosis in a mixed dairy farm and evaluation of a test and slaughter strategy to release the herd out of the quarantine

Cited by 9 publications

References 57 publications

The perspective of antibiotic therapeutic challenges of brucellosis in the Middle East and North African countries: Current situation and therapeutic management

The perspective of antibiotic therapeutic challenges of brucellosis in the Middle East and North African countries: Current situation and therapeutic management

Cross-sectional study and Building a Geographical Information System for Brucellosis in Monufiya

Prevalence and Molecular Differentiation of Brucella Species ‎in Buffaloe Raw Milk and Karish Cheese in Egypt

Contact Info

Product

Resources

About