Investigation of anodized titanium implants coated with triterpenoids extracted from black cohosh: an animal study

Abstract

PURPOSE.The purpose of this study was to evaluate bone response to anodized titanium implants coated with the extract of black cohosh, Asarum Sieboldii, and pharbitis semen. MATERIALS AND METHODS. Forty anodized titanium implants were prepared as follows: group 1 was for control; group 2 were implants soaked in a solution containing triterpenoids extracted from black cohosh for 24 hours; group 3 were implants soaked in a solution containing extracts of black cohosh and Asarum Sieboldii for 24 hours; group 4 were implants soaked in a solution containing extracts of pharbitis semen for 24 hours. The implants from these groups were randomly and surgically implanted into the tibiae of ten rabbits. After 1, 2, and 4 weeks of healing, the nondecalcified ground sections were subjected to histological observation, and the percentage of bone-to-implant contact (BIC%) was calculated. RESULTS. All groups exhibited good bone healing with the bone tissue in direct contact with the surface of the implant. Group 2 (52.44 ± 10.98, 25.54 ± 5.56) showed a significantly greater BIC% compared to that of group 3 (45.34 ± 5.00, 22.24 ± 2.20) with respect to the four consecutive threads and total length, respectively. The BIC% of group 1 (25.22 ± 6.00) was significantly greater than that of group 3 (22.24 ± 2.20) only for total length. CONCLUSION. This study did not show any remarkable effects of the extract of black coshosh and the other natural products on osseointegration of anodized titanium implants as coating agents. Further studies about the application method of the natural products on to the surface of implants are required. [ J Adv Prosthodont 2014;1:14-21]

Promoting Effects of Titanium Implants Coated with Dipterocarpus tuberculatus Extract on Osseointegration

“…Under these processes, the expression of several key genes, including COL1a1 , ALP , OPN , and Runx2 , were significantly enhanced and multiple signaling pathways including RANKL-RANK-induced signaling and TGF-β signaling were activated . However, anodized Ti implants coated with the extract of black cohosh, Asarum sieboldii, and Pharbitis semen did not stimulate osseointegration in the tibia of rabbits after implantation for 4 weeks . Additionally, some natural products administered in the oral tracts exhibit the promoting effects of osseointegration.…”

“…32 However, anodized Ti implants coated with the extract of black cohosh, Asarum sieboldii, and Pharbitis semen did not stimulate osseointegration in the tibia of rabbits after implantation for 4 weeks. 33 Additionally, some natural products administered in the oral tracts exhibit the promoting effects of osseointegration. Oral administration of avocado/soybean unisaponifiables for 60 d improved osseointegration in the tibia of rats with experimental arthritis.…”