Investigation of Anticancer Activity of Lindernia crustacea (L.) F. Muell. var. Crustacean

Barusrux, Sahapat; Weerapreeyakul, Natthida; Phiboonchaiyanan, Preeyaporn Plaimee; Khamphio, Munthipha; Tanthanuch, Waraporn; Thummanu, Kanjana

doi:10.48048/wjst.2019.6290

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…Only YKH ethanolic extract, but not YKH aqueous extract showed potent anticancer activity on many human cancer cell lines such as colon cancer cell line (HCT116), liver cancer cell (HepG2) and Jurkat leukemic T cell. However, YKH ethanolic extract has less toxicity on human lung cancer cell line (SK-LU1) and non-toxicity on African green monkey kidney cell (Vero normal cell line) [2]. There has been no previous information reported about mechanism of YKH anticancer activity.…”

Section: Introductionmentioning

confidence: 99%

Anticancer Activity of Lindernia crustacea (L.) F. Muell. var. Crustacean on Human HCT116 Colon Cancer Cell via Cellular Lipid and β-sheet Protein Accumulation

Barusrux

Weerapreeyakul

Phiboonchaiyanan

et al. 2020

Walailak J Sci & Tech

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Lindernia crustacea (L.) F. Muell. var. crustacean or “Ya Kap Hoi: YKH”, which is an edible vegetable, is commonly seen in Thailand. This study investigated its anticancer properties with high antioxidant activity by reducing power activity, excluding alkylation activity. It explored YKH extract induction of anticancer activity through biomolecular changes in the HCT116 human colon cancer cell line. The ethanolic extract stock solution and water extract stock solution were prepared. NR assay was used for cancer cell cytotoxic testing and Fourier transform infrared (FTIR) microspectroscopy was used for biomolecular changes study on lipid, protein and nucleic acid/DNA. The apoptosis induction by the extracts was detected by using Annexin V-FITC and DAPI staining. The compounds in the YKH ethanolic extract was performed by using GC-MS analysis. As a result, YKH ethanolic extract caused HCT116 colon cancer cell death in the dose dependent manner after 24 h exposure, and the 50 % cell death (IC50 concentration) was 195.4 ± 12 μg/mL. Cellular biochemical changes observed from FTIR data showed that YKH ethanolic extract treated HCT116 colon cancer cell. There is an increase in lipid content and a reduction in intensity of nucleic acid/DNA, a-helix protein structure at 1,656 cm-1 was reduced and peak position of b-sheet structure (1,637 cm-1) was shifted to lower frequency. From the analysis results, YKH ethanolic extract seems to exert anti-colon cancer effect by changing cellular biomolecular structure of lipids and β-sheet protein accumulation, supporting apoptotic induction. The compounds in the YKH ethanolic extract mainly yielded fatty acids,which may be useful as potential compounds.

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Section: Introductionmentioning

confidence: 99%

Anticancer Activity of Lindernia crustacea (L.) F. Muell. var. Crustacean on Human HCT116 Colon Cancer Cell via Cellular Lipid and β-sheet Protein Accumulation

Barusrux

Weerapreeyakul

Phiboonchaiyanan

et al. 2020

Walailak J Sci & Tech

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“…It is found to be an effective against various rodents [13] . It is also reported to show anticancer [14] and antioxidant [15] activities. Many other species of the same family have been reported for antimicrobial and anthelminthic activity.…”

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Phytochemical Investigation, Liquid Chromatography-Mass Spectrometry Analysis, Antibacterial and Anthelminthic Activity of Lindernia crustacea (L.) F. Muell.

Ghori¹,

Tehseen²,

Sultana³

et al. 2021

ijps

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Lindernia crustacea (L.) F. Muell is one of the commonly used medicinal plants native to India, Indonesia and Malaysia. In the present investigation antibacterial activity of leaf and stem petroleum ether and methanolic extracts was performed at 25 µg/ml, 50 µg/ml, 75 µg/ml and 100 µg/ml concentrations and anthelminthic activity of leaf methanolic extract was performed at 25 µg/ml, 75 µg/ml and 100 µg/ml concentrations. The powdered leaf and stem were extracted by soxhlation and the phytochemical screening of the extracts was performed for detection of phytoconstituents. The leaf petroleum ether and methanolic extracts showed the presence of alkaloids, carbohydrates, tannins and glycosides, whereas the stem petroleum ether and methanolic extracts showed alkaloids, carbohydrates, flavonoids and tannins. Antibacterial activity was performed by cup and plate method using Staphylococcus aureus and Escherichia coli with Penicillin G as standard. Antibacterial activity was not found in both stem and leaf extracts. Anthelminthic activity was performed using Indian earthworm, with albendazole as standard drug. The leaf methanolic extract showed significant anthelminthic activity at 100 µg/ml with paralysis time of 58±3.978 and death time of 79±4.796 as compared with standard drug. The anthelminthic activity of methanolic extract might be due the presence of phytoconstituents as identified by liquid chromatography-mass spectrometry analysis.

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Antioxidant and Antiproliferative Activity of Selected Medicinal Plants of Lower Assam, India: An In Vitro and In Silico Study

Swargiary

Verma

Singh³

et al. 2020

ACAMC

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Background: The use of medicinal plants for general wellbeing and disease treatment is a common practice among tribal communities of Kokrajhar districts of Assam. However, little works have been done to study the pharmacological aspect of the plants. Objectives: The present study intends to study the antioxidant and antiproliferative properties of selected medicinal plants used by the tribal communities of Kokrajhar district of Assam since ancient times. Methods: Five traditionally important medicinal plants namely, Cassia fistula, Citrus grandis, Lindernia crustacea, Sacciolepis myosuroides, and Zingiber zerumbet were investigated for antioxidant, antiproliferative (cytotoxic) and apoptosis-inducing potential in the malignant cancer cell line. Phytochemical content such as phenolic and flavonoid content were estimated following standard protocol. The methanolic extract of plants were investigated following phosphomolybdate method (TAC), FRAP, DPPH, ABTS, and TBARS assays. Antiproliferative activities of the plants were carried out by MTT assay in DL and PBMC cells. The apoptotic study was carried out following the acridine orange and ethidium bromide staining method and fluorescent microscopic imaging. Based on the significant (P≤0.05) high apoptotic inducing potential of the plant and to further dissect the molecular mode of action including downstream biological action, major phytochemicals derived from L. crustacea were investigated for its prospective binding affinity with antiapoptotic cancer target proteins. Results: Antioxidant studies by FRAP, DPPH, ABTS, and TBARS assay revealed that all the five plants contain considerable free radical scavenging activity. C. fistula showed the strongest free radical scavenging activity while the fruit peel extract of C. grandis showed poor activity. The overall antioxidant activities of plants such as TAC, FRAP, DPPH, ABTS, and TBARS may be arranged in decreasing activity as C. fistula > Z. zerumbet > L. crustacea > S. myosuroides > C. grandis. MTT based cell proliferation study showed that all the plants extract significantly (P≤0.05) inhibited cell viability with negligible cytotoxicity (~5-12%) in normal cells. Moreover, L. crustacea showed promising antiproliferative and apoptosis-inducing ability against Dalton’s lymphoma. It is worth mentioning that the major bioactive compounds of the most potent plant extract, L. crustacea interacted with anti-apoptotic proteins (cancer target) with higher affinity and the results are compared with reference inhibitors. Conclusion: It is worth noting that these plants have the potential to consider for further scientific studies in different cell lines and animal models. Furthermore, isolation and characterization of bioactive compound(s), may promise the discovery of new and valuable drugs candidate to tackle various human diseases.

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Investigation of Anticancer Activity of Lindernia crustacea (L.) F. Muell. var. Crustacean

Cited by 3 publications

References 19 publications

Anticancer Activity of Lindernia crustacea (L.) F. Muell. var. Crustacean on Human HCT116 Colon Cancer Cell via Cellular Lipid and β-sheet Protein Accumulation

Anticancer Activity of Lindernia crustacea (L.) F. Muell. var. Crustacean on Human HCT116 Colon Cancer Cell via Cellular Lipid and β-sheet Protein Accumulation

Phytochemical Investigation, Liquid Chromatography-Mass Spectrometry Analysis, Antibacterial and Anthelminthic Activity of Lindernia crustacea (L.) F. Muell.

Antioxidant and Antiproliferative Activity of Selected Medicinal Plants of Lower Assam, India: An In Vitro and In Silico Study

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