Investigation of Antimicrobial Activity of Pyocyanin Produced by Pseudomonas aeruginosa Strains Isolated from Different Clinical Specimens

Abstract

Ö Z E T P seudomonas aeruginosa suşları tarafından üretilen piyosiyanin karakteristik mavi-yeşil bir fenazin pigmentidir. Çalışmanın amacı, P. aeruginosa suşlarında en yüksek piyosiyanin üretiminin belirlenmesi ve piyosiyaninin Escherichia coli, Bacillus sp., Pseudomonas aeruginosa, Candida sp. and Aspergillus niger üzerindeki antimiktobiyal etkisinin araştırılmasıdır. Bu doğrultuda, piyosiyanin P. aeruginosa kültür süpernatanından özütlendi ve saflaştırıldı, ardından spektrofotometrik olarak ölçüldü. En yüksek piyosiyanin üretiminin idrar örneğinden izole edilen P. aeruginosa A10 suşunda ve en düşük piyosiyanin üretiminin batından izole edilen P. aeruginosa A1 suşunda olduğu belirlendi. Piyosiyaninin antimikrobiyal etkisinin belirlenmesinde 'Agar Kuyu Difüzyon' yöntemi uygulandı. Böylece, piyosiyanin Bacillus sp., Escherichia coli ve Candida sp., üzerinde etkili bir antimikrobiyal ajanken, P. aeruginosa ve A. niger üzerinde antimikrobiyal etkisi görülmedi. Ardından, agar kuyu yöntemi ile piyosiyanine duyarlı olduğu belirlenen suşlara 'Tüp Seyreltme Yöntemi' kullanıldı. Azalan piyosiyanin derişimleri ile Bacillus sp. and Candida sp. suşlarının üremesinde artış gözlendi. Anahtar KelimelerPseudomonas aeruginosa, Piyosiyanin, Antimikrobiyal Aktivite. A B S T R A C TP yocyanin is the characteristic blue-green phenazine pigment produced by Pseudomonas aeruginosa strains. The aim of our study is to determine the highest amount of pyocyanin production in P. aeruginosa strains and to investigate the antimicrobial effect of pyocyanin pigment on Escherichia coli, Bacillus sp., Pseudomonas aeruginosa, Candida sp. and Aspergillus niger. For this purpose, pyocyanin was extracted from P. aeruginosa culture supernatants and purified, and then it was measured by spectrophotometrically. P. aeruginosa A10 strain isolated from urine specimen was determined as the highest amount of pyocyanin producer and P. aeruginosa A1 strain isolated from abdomen was the lowest. The 'Agar Well Diffusion' method was carried out to determine the antimicrobial effect of pyocyanin. Accordingly, pyocyanin was effective as an antimicrobial agent on Bacillus sp., Escherichia coli and Candida sp., but there was no antimicrobial effect on P. aeruginosa and A. niger. After that 'Tube Dilution Method' was used on strains, which were determined as sensitive to pyocyanin with agar well diffusion method. An increase in the intensity of Bacillus sp. and Candida sp. strains' growth with decreasing the concentration of pyocyanin was observed.

Isolation and characterization of nutrient dependent pyocyanin from Pseudomonas aeruginosa and its dye and agrochemical properties

“…Abdul-Hussein & Atia 34 reported that the growth of Escherichia coli and of Aspergillus fumigatus were inhibited in the range between 10 to 100 μg ml −1 , while the growth of yeast Cryptococcus neoformans was equally inhibited by pyocyanin. In another study, E. coli, Bacillus sp., and Aspergillus niger strains were found to be sensitive to the phenazin pigment pyocyanin at 0.005 to 50 µg as analyzed by the tube dilution and agar well diffusion methods 35 . The antagonistic activity of pyocyanin was studied at low concentration of 100 µL against Macrophomina phaseolina plant pathogenic fungus that is responsible for stem rot, seedling blight and root rot.…”

Size and Shape Directed Novel Green Synthesis of Plasmonic Nanoparticles Using Bacterial Metabolites and Their Anticancer Effects

“…The siderophore pyoverdine was isolated from P. aeruginosa 25W using a previously reported method ( Özyürek et al, 2016 ). Briefly, the bacterial seed culture was grown in an Luria-Bertani (LB) medium for a period of 12 h. The actively growing seed culture (O.D.…”

“…Pyoverdine and cyclodipeptides are prominent examples from Pseudomonas aeruginosa and help bacteria to chelate iron and facilitate quorum sensing ( Visca et al, 2007 ). Additionally, P. aeruginosa synthesizes water diffusible, blue-green colored pigment, pyocyanin, which shows antibacterial properties ( Crosa and Walsh, 2002 ; Özyürek et al, 2016 ). In this work we demonstrated rapid, synthesis of anisotropic gold and silver PNPs using pyoverdine and pyocyanin obtained from an indigenously isolated strain of P. aeruginosa ( Mavrodi et al, 2001 ).…”

Colour Me Blue: The History and the Biotechnological Potential of Pyocyanin

“…The two greatest potential perspectives for the use of pyocyanin are in industry or in pharmacology, given its antimicrobial nature. The bioprospecting of pyocyanin is based on the fact that the pigment is an active redox compound, i.e., an extracellular electron carrier agent, with NADH as the initial endogenous and molecular reducer [ 107 , 128 ]. Microbial fuel cells (MFC) are devices that convert metabolic energy into electrical current using P. aeruginosa or other bacteria [ 170 ].…”