Investigation of aqueous extract of Moringa oleifera lam seed for antiviral activity against newcastle disease virus in ovo

Chollom, S. C.; Agada, G. O. A.; Gotep, Jurbe Gofwan; Mwankon, S E; Dus, P C; Bot, YS; Nyango, D Y; Singnap, Changjul L.; Fyaktu, E J; Okwori, Aej

doi:10.5897/jmpr12.394

Cited by 32 publications

(29 citation statements)

References 15 publications

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“…This research shows that the study plant may emanate as an alternative approach to prophylactic and therapeutic effect of viral infection which is in agreement with studies conducted by Chollom et al (2012) and Bakari et al (2013) that showed the anti-NDV potentials…”

Section: Resultssupporting

confidence: 77%

In-ovo biological activities of Phyllanthus amarus leaf extracts against Newcastle disease virus

Faeji¹,

Oladunmoye²,

Adebayo³

et al. 2017

J. Med. Plants Res.

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Newcastle disease virus is a paramyxovirus which causes Newcastle disease in birds. Investigation was done on the effect of leaf extract of Phyllanthus amarus against Newcastle disease virus (NDV) using an in-ovo assay. Nine to eleven day-old viable embryonated chicken eggs (ECE) were used for the assay, these were divided into six groups of six eggs each. Methanol, aqueous and n-hexane extracts of the plant leaves were administered to the various groups at concentrations varying from 50 to 5 mg/ml. Embryonated eggs were incubated and embryo survival was monitored daily. Negative control and diluents control groups received phosphate buffer saline (PBS) and dimethly suphoxide (DMSO), respectively. The other group was uninoculated while a virus control group received 100 EID 50 /0.1 ml NDV alone. Bacteria free allantoic fluid from the embryonated eggs in different treatment groups were harvested and collected for spot hemagglutination (HA) test and HA assay to detect the presence of NDV viral particles and the viral titre, respectively. Leaf extracts were assayed for presence of phytochemicals and antioxidant potentials. It was observed from the results that the extract was toxic to the embryo at a concentration above 50 mg/ml and further results showed that the HA viral titre reduction was directly proportional to increasing extract concentration. The phytochemical assays of leaf extract revealed the presence of phytochemicals including alkaloids, tannins, saponins, flavonoids, phenols, steroids, glycosides. The current findings have demonstrated that leaf extract from P. amarus has potentials of medicinal value as well as antiviral activity against NDV in-ovo. Further experimental assays using live animal models are recommended to validate the use of P. amarus plant extract in therapeutic measure in chickens.

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Section: Resultssupporting

confidence: 77%

In-ovo biological activities of Phyllanthus amarus leaf extracts against Newcastle disease virus

Faeji¹,

Oladunmoye²,

Adebayo³

et al. 2017

J. Med. Plants Res.

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“…The study of Yang et al (2006) showed that dietary dehydrated leaves of Moringa induce a significant increase in the concentrations of total globulin, γ-globulin and lymphocyte ratio, antibody titer levels with comparison to the standard (sheep erythrocytes), and delayed-type hypersensitivity of broiler chickens. Comparable results also were obtained by Chollom et al (2012), Eze et al (2013), Hossam et al (2016) and Younis and Elbestawy (2017) who demonstrated that M. oleifera extracts have nutritional value as well as strong antiviral activity against ND virus. Means with different superscripts, within age, are significantly different (P < 0.05)…”

Section: Resultssupporting

confidence: 54%

Influence of dietary Moringa oleifera on broilers performance, intestinal microbial population and humoral immune competence

El-Ghany

Eraky

2020

J Hellenic Vet Med Soc

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This study was designed to evaluate the effect of using different levels of phytobiotic containing Moringa oleifera (M. oleifera) leaf powder (MOLP) on broiler chickens body growth performance parameters, intestinal microbial population, and the humoral immune response. Day-old Hubbard broiler chicks (n = 200) were randomly allocated into 4 treatment diet. Basal diet was supplied with treatments T0, T1, T2 and T3 representing (0, 1%, 5% and 7.5% MOLP); respectively. Chickens were kept under observation for 5 weeks. Body performance parameters, total viable bacterial and coliform counts and humoral immune response to Newcastle disease (ND) virus vaccine were detected using the haemagglutination inhibition (HI) test. The results revealed significant (P < 0.05) improvement in performance parameters in groups supplemented with dietary MOLP. However, the best significant (P < 0.05) performance was observed in the group fed 1% MOLP. There was no significant (P < 0.05) difference among treatments considering the total viable intestinal bacterial and coliform counts. Significant (P < 0.05) increase in the means of HI in dietary MOLP supplemented groups was observed, where the highest means were seen in 1% MOLP treated birds. In conclusion, dietary supplementation with MOLP could improve the performance parameters and the immune response while reducing the total viable intestinal bacterial and coliform counts of broiler chickens. The study recommended using of dietary level of 1% MOLP to improve performance, intestinal health, and immune competence.

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“…Also, Eze et al (2013) indicated that methanolic extract of M. oleifera at 200 mg/kg body weight increased ND HI titer in the vaccinated and un-vaccinated chicken groups and it could be recommended as an immune-booster treatment against ND in non-vaccinated birds. In the same trend, Chollom et al (2012) revealed that M. oleifera seed extract had a strong antiviral activity against ND in ovo. Du et al (2007) reported that supplementation of MOLM resulted in significantly increased immune response of broilers.…”

Section: Immune Responsementioning

confidence: 61%

Impact of Supplementation of Moringa Oleifera in Diet of Broiler Chicks on Their Behavior, Welfare, Performance and Immune Responses

Ramadan¹

2017

AJVS

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A total of two hundred and four, one day old Ross broiler chicks were randomly allotted into four dietary treatments floor pen groups (17 birds/ pen, 10 birds/m 2 ) with three replicates for each treatment. The dietary treatments were designed to feed the chick's basal diet supplemented with graded levels of Moringa oleifera leaves meal, MOLM [3%, 5%, 8% and 0% (control) MOLM]. Behavioral observation was carried out twice daily (AM,(7)(8)(9)(10)(13)(14)(15) two days a week for 6 consecutive weeks. Body weight (BW), Body weight gain (BWG) and Feed intake per pen was measured weekly, from which feed conversion ratio (FCR) was calculated. At 30 th day of age blood samples were collected to evaluate anti body titer (HI) against New Castel Diseased Virus (ND). At the end of the experiment, fear responses, differential leucocyte count, Hb and PCV were assayed as well as some carcass traits. Also, mortality rate was estimated. The obtained results showed that higher proportion of birds were engaged in feeding in groups dietary treated with 3% and 5% MOLM, while intermediate responses was shown in birds supplemented with 8% MOLM compared to control ones. The supplemented groups with MOLM showed a lower walking and standing activities than non-supplemented ones. Dietary supplementation with different levels MOLM significantly improved BW, BWG and FCR. In addition, the mortality rates for the dietary treated groups were not affected. The MOLM supplemented groups were less fearful in tonic immobility test, less stressed and had lower heterophil/lymphocyte ratio when compared to un-supplemented ones. MOLM had no effect on Hb concentration and carcass traits. PCV was significantly higher in birds fed 5% MOLM compared to control birds. Birds supplemented with 3%, 5% and 8% MOLM had significant higher antibody titre against ND compared to un-supplemented birds. In conclude, dietary supplementation of broiler chicks with MOLM up to 8% resulted in improved welfare, performance and immune response against ND.

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Investigation of aqueous extract of Moringa oleifera lam seed for antiviral activity against newcastle disease virus in ovo

Cited by 32 publications

References 15 publications

In-ovo biological activities of Phyllanthus amarus leaf extracts against Newcastle disease virus

In-ovo biological activities of Phyllanthus amarus leaf extracts against Newcastle disease virus

Influence of dietary Moringa oleifera on broilers performance, intestinal microbial population and humoral immune competence

Impact of Supplementation of Moringa Oleifera in Diet of Broiler Chicks on Their Behavior, Welfare, Performance and Immune Responses

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