Investigation of Aspergillus flavus in animal virulence

Lan, Huahui; Wu, Lianghuan; Sun, Rong; Yang, Ke; Liu, Yinghang; Wu, Jiefei; Geng, Longpo; Huang, Chuanbing; Wang, Shihua

doi:10.1016/j.toxicon.2018.02.043

Cited by 10 publications

(8 citation statements)

References 56 publications

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“…Using an invertebrate model of fungal disease, we evaluated virulence for all strains. A. fumigatus virulence assays typically use concentrations of 1 × 10 6 conidia (asexual spores) to inoculate Galleria mellonella larvae ( 41 , 42 ). When we inoculated larvae with section Flavi species at 1 × 10 6 , all animals died within 2 days.…”

Section: Resultsmentioning

confidence: 99%

Genomic and Phenotypic Trait Variation of the Opportunistic Human Pathogen Aspergillus flavus and Its Close Relatives

et al. 2022

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Aspergillus flavus is a filamentous fungus that causes opportunistic human infections, such as aspergillosis and fungal keratitis, but its close relatives are considered nonpathogenic. To begin understanding how this difference in pathogenicity evolved, we characterized variation in infection-relevant genomic, chemical, and phenotypic traits between strains of A. flavus and its relatives.

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Section: Resultsmentioning

confidence: 99%

Genomic and Phenotypic Trait Variation of the Opportunistic Human Pathogen Aspergillus flavus and Its Close Relatives

et al. 2022

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“…The data of threshold cycle (Ct) were analyzed with unpaired t -tests using GraphPad Prism 5.01 (GraphPad Software, La Jolla, CA, United States). In order to confirm this model represent the virulence of the fungus A. flavus , microscopic and histological changes of mice lung tissues were observed after infection using a previously described method (Lan et al, 2018). The whole lungs of infected mice were putted in 10% paraformaldehyde, and then stained with Haema eosin (HE) for histologic examination.…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization of a Debilitation-Associated Partitivirus Infecting the Pathogenic Fungus Aspergillus flavus

Jiang

Wang

et al. 2019

Front. Microbiol.

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The opportunistic human pathogenic fungus Aspergillus flavus is known to be infected with mycoviruses. In this study, we report a novel mycovirus A. flavus partitivirus 1 (AfPV1) that was originally isolated from the abnormal colonial morphology isolate LD-3-8 of A. flavus . AfPV1 has spherical virus-like particles about 40 nm in diameter, and three double-stranded RNA (dsRNA) segments (dsRNA1, 2, and 3 with lengths of 1.7, 1.4, and 1.1 kbp, respectively) were packaged in the virions. dsRNA1, dsRNA2, and dsRNA3 each contained a single open reading frame and potentially encoded 62, 42, and 32 kDa proteins, respectively. The dsRNA1 encoded protein shows similarity to the RNA-dependent RNA polymerase (RdRp) of partitiviruses, and the dsRNA2 product has no significant similarity to any other capsid protein (CP) in the GenBank databases, beside some homology with the hypothetical “capsid” protein of a few partitiviruses. The dsRNA3 encodes a protein with no similarity to any protein in the GenBank database. SDS-PAGE and polypeptide mass fingerprint-mass spectrum (PMF-MS) analyses indicated that the CP of the AfPV1 was encoded by dsRNA2. Phylogenetic analysis showed that the AfPV1 and relative viruses were found in an unclassified group inside the Partitiviridae family. AfPV1 seems to result in debilitation symptoms, but had no significant effects to murine pathogenicity. These findings provide new insights into the partitiviruses taxonomy and the interactions between viruses and A. flavus .

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“…Aspergillus flavus, one of the most abundant soil saprophytic fungi on Earth, contaminates many agricultural crops, such as maize, cotton and peanuts, causing huge economic losses (Amaike and Keller, 2011). Additionally, A. flavus is also an opportunistic pathogen that causes aspergillosis or liver cancer in animals and humans (Lan et al, 2018). Aflatoxins, which are mainly produced by A. flavus and Aspergillus parasiticus, were found to be one of the most toxic and carcinogenic secondary metabolites (Yang et al, 2018a).…”

Section: Introductionmentioning

confidence: 99%

Lysine acetylation contributes to development, aflatoxin biosynthesis and pathogenicity in Aspergillus flavus

Guang

Yue

Ren

et al. 2019

Environmental Microbiology

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Summary Aspergillus flavus is a pathogenic fungus that produces carcinogenic aflatoxins, posing a great threat to crops, animals and humans. Lysine acetylation is one of the most important reversible post‐translational modifications and plays a vital regulatory role in various cellular processes. However, current information on the extent and function of lysine acetylation and aflatoxin biosynthesis in A. flavus is limited. Here, a global acetylome analysis of A. flavus was performed by peptide pre‐fractionation, pan‐acetylation antibody enrichment and liquid chromatography–mass spectrometry. A total of 1313 high‐confidence acetylation sites in 727 acetylated proteins were identified in A. flavus. These acetylation proteins are widely involved in glycolysis/gluconeogenesis, pentose phosphate pathway, citric acid cycle and aflatoxin biosynthesis. AflO (O‐methyltransferase), a key enzyme in aflatoxin biosynthesis, was found to be acetylated at K241 and K384. Deletion of aflO not only impaired conidial and sclerotial developments, but also dramatically suppressed aflatoxin production and pathogenicity of A. flavus. Further site‐specific mutations showed that lysine acetylation of AflO could also result in defects in development, aflatoxin production and pathogenicity, suggesting that acetylation plays a vital role in the regulation of the enzymatic activity of AflO in A. flavus. Our findings provide evidence for the involvement of lysine acetylation in various biological processes in A. flavus and facilitating in the elucidation of metabolic networks.

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Investigation of Aspergillus flavus in animal virulence

Cited by 10 publications

References 56 publications

Genomic and Phenotypic Trait Variation of the Opportunistic Human Pathogen Aspergillus flavus and Its Close Relatives

Genomic and Phenotypic Trait Variation of the Opportunistic Human Pathogen Aspergillus flavus and Its Close Relatives

Molecular Characterization of a Debilitation-Associated Partitivirus Infecting the Pathogenic Fungus Aspergillus flavus

Lysine acetylation contributes to development, aflatoxin biosynthesis and pathogenicity in Aspergillus flavus

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