Investigation of bcr1 Gene Expression in Candida albicans Isolates by RT-PCR Technique and its Impact on Biofilm Formation

Nikoomanesh, Fatemeh; Roudbarmohammadi, Shahla; Roudbary, Maryam; Bayat, Mansour; Heidari, Ghasem

doi:10.18869/modares.iem.2.1.22

Cited by 13 publications

(10 citation statements)

References 16 publications

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“…This finding suggests that C. parapsilosis sensu stricto during biofilm growth, would express more or less Bcr1 depending on its formative capacity. This result is compatible with the study by NiKoomanesh et al, who also demonstrated a positive relationship between the expression of the BCR1 gene and the formation of biofilm in isolates of Candida albicans [34]. Either way, both our results and those of Pannanusorn et al [29], locate BCR1 as a key regulator of biofilm production in this fungal model.…”

Section: Discussionsupporting

confidence: 93%

The oral cavity promotes virulence of Candida parapsilosis sensu stricto via up-regulation of BCR1

Lourdes¹,

Sabrina²,

Cristin³

et al. 2020

World J. Adv. Res. Rev.

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Section: Discussionsupporting

confidence: 93%

The oral cavity promotes virulence of Candida parapsilosis sensu stricto via up-regulation of BCR1

Lourdes¹,

Sabrina²,

Cristin³

et al. 2020

World J. Adv. Res. Rev.

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“…Out of these three biofilm-related genes, BCR1 was significantly upregulated in biofilms of all C. parapsilosis complex isolates relative to the planktonic cells which revealed that this gene might be responsible for biofilm formation in C. parapsilosis species complex. On the same note, Nikoomanesh et al [47] biofilm formation in C. albicans isolates. Moreover, Pannanusorn et al [46] suggested that biofilm formation in C. parapsilosis isolates is both dependent and independent on BCR1 gene.…”

Section: Discussionmentioning

confidence: 91%

Antifungal susceptibility pattern and biofilm-related genes expression in planktonic and biofilm cells of Candida parapsilosis species complex

Modiri¹,

Hashemi²,

Ghazvini³

et al. 2019

CMM

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Background and Purpose: Candida parapsilosis complex isolates are mainly responsible for nosocomial catheter-related infection in immunocompromised patients. Biofilm formation is regarded as one of the most pertinent key virulence factors in the development of these emerging infections. The present study aimed to compare in vitro antifungal susceptibility patterns and biofilm-related genes expression ratio in planktonic and biofilm’s cells of clinically C. parapsilosis complex isolates.Materials and Methods: The current study was conducted on a number of 17 clinical C. parapsilosis complex (10 C. parapsilosis sensu stricto, 5 C. orthopsilosis, and 2 C. metapsilosis). The antifungal susceptibility patterns of amphotericin B, fluconazole, itraconazole, voriconazole, posaconazole, and caspofungin in planktonic and biofilm forms were closely examined using CLSI M27-A3 broth microdilution method. The expression levels of biofilm-related genes (BCR1, EFG1, and FKS1) were evaluated in planktonic and biofilm’s cells using Real-time polymerase chain reaction (PCR) technique.Results: The obtained results indicated that all C. parapsilosis complex isolates were able to produce high and moderate amounts of biofilm forms. In addition, the sessile minimum inhibitory concentrations were reported to be high for fluconazole (≥ 64 μg/ml), itraconazole, voriconazole, and posaconazole (≥ 16 μg/ml), as compared to planktonic minimum inhibitory concentrations. Moreover, a significant difference was observed between antifungal susceptibility patterns for all azole antifungal agents (P<0.05). Furthermore, the BCR1 overexpression was considered significant in biofilms with regard to planktonic cells in C. parapsilosis species complex (P=0.002).Conclusion: C. parapsilosis complex isolates were found susceptible to most of the tested antifungal drugs, while biofilms demonstrated a noticeable resistant to azoles. The marked discrepancy noted in antifungal susceptibility patterns among these species should be highlighted to achieve effective therapeutic treatment.

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“…The expression of Sap6, HWP1 and Rim101 genes was evaluated using q RT-PCR. Here, fresh culture colonies of C. albicans (10 3 cells/ml) were counted and treated with a 300 mM concentration of farnesol and nanogels containing farnesol and incubated for 24 h at 37 C. After this time, yeast cells were harvested and washed with PBS, then, total RNA from treated and non-treated cells was extracted using glass bead and lysis buffer as described previously [28,29]. Then, cDNA was synthesized using vivantis kit (Subang Jaya, Malaysia) as recommended by the manufacturer's protocol.…”

Section: Quantitative Real-time Polymerase Chain Reaction (Qrt-pcr)mentioning

confidence: 99%

Design and synthesis of mucoadhesive nanogel containing farnesol: investigation of the effect on HWP1, SAP6 and Rim101 genes expression of Candida albicans in vitro

Nikoomanesh

Roudbarmohammadi

Khoobi

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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The evolution of drug resistance of Candida species to conventional antifungal agents has been a major medical challenge worldwide; attempt to use the potential antifungal agents with appropriate therapy efficacy and minimum effects is considerably growing. This study was conducted to evaluate the use of nanogel as a nanocarrier for pharmaceutical application of farnesol. The nanogels were synthetized using alginate (AL) and chitosan (CS) polymers containing 300 mM of farnesol in the nanorange 42-70 nm size. In vitro release studies indicated that release of farnesol from CS and AL nanogels was as 58% and 37%, respectively. Chitosan nanogel showed more in inhibitory zone as compared to AL nanogel (9 mm). Also, cytotoxicity assay showed no significant difference between control and treatment groups (p>.05). Finally, the effect of nanogels on genes expression of HWP1, SAP6 and Rim101 in Candida albicans ATCC10231 was assessed using real-time polymerase chain reaction (PCR). Expression of HWP1 and SAP6 genes in C. albicans treated with CS nanogel was significantly decreased (p<.01). In general, the obtained finding showed that, CS nanogel contains farnesol with proper antifungal activity and as a new approach used in pharmaceutical applications against C. albicans; however, more studies in vitro and in vivo are needed in the future.

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Investigation of bcr1 Gene Expression in Candida albicans Isolates by RT-PCR Technique and its Impact on Biofilm Formation

Cited by 13 publications

References 16 publications

The oral cavity promotes virulence of Candida parapsilosis sensu stricto via up-regulation of BCR1

The oral cavity promotes virulence of Candida parapsilosis sensu stricto via up-regulation of BCR1

Antifungal susceptibility pattern and biofilm-related genes expression in planktonic and biofilm cells of Candida parapsilosis species complex

Design and synthesis of mucoadhesive nanogel containing farnesol: investigation of the effect on HWP1, SAP6 and Rim101 genes expression of Candida albicans in vitro

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