Investigation of biomembrane-drug interactions of different biomembranes by capillary electrophoresis

Wei, ChunLiang; Yang, Gengliang; Shang, Chaoqun; Landi, Giulia; Jia, MiaoHui

doi:10.1007/s11434-009-0186-3

Cited by 2 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different types of biomembranes including mice red cell membrane, S-180 cell membrane, and human red cell membrane were coated onto the capillary inner wall to develop biomembrane-based capillaries. 26 During the preparation process, glycidochloro-propyl methyl trimethoxy saline (HG-560) and tetramethyl orthosilicate (TMOS) were selected as precursors to develop sol−gel open tubular columns. Then, the biomembrane suspension was pumped into the capillary at room temperature.…”

Section: ■ Microscale Systems "See" In Cellsmentioning

confidence: 99%

What the Microscale Systems “See” In Biological Assemblies: Cells and Viruses?

et al. 2021

View full text Add to dashboard Cite

Section: ■ Microscale Systems "See" In Cellsmentioning

confidence: 99%

What the Microscale Systems “See” In Biological Assemblies: Cells and Viruses?

et al. 2021

View full text Add to dashboard Cite

CCR4 expressing cells cultured adherently on a capillary wall and formaldehyde fixed as the stationary phase for ligand screening by ACE

Yakufu

et al. 2013

Electrophoresis

View full text Add to dashboard Cite

We have developed an on-line screening method for CC chemokine receptor 4 (CCR4) ligands, in which the whole cells expressed with CCR4 were cultured adherently and immobilized on the inner wall of the capillary as the stationary phase for the first time. Moreover, in this method it is unnecessary to isolate and purify the target receptors from cell membranes. Therefore, it is possible to almost completely preserve the native conformation of the target receptors. The binding activities of the immobilized CCR4 did not change. A known antagonist of CCR4, compound A, was employed to validate the bioactivity of the cell layer and stability of this method. The intraday, interday, and batch-to-batch reproducibilities were investigated (RSD ≤ 13.9%). Nonlinear chromatography was used to calculate the binding constant between the compound A and CCR4 (6.4 × 10(4)/M, RSD = 4.96%). Using this method, the qualitative and quantitative characterizations of 23 computer-aided drug design compounds were achieved and the kinetic parameters (K, k(a), k(d), and k') were obtained by nonlinear chromatography. Three active compounds were screened out, which also showed activity in chemotaxis inhibition assay. The experimental results show that this method is simple, sensitive, and efficient for drug screening. Moreover, it offered a novel way to detect the nonspecific interactions between ligands and cell membrane.

show abstract

Investigation of biomembrane-drug interactions of different biomembranes by capillary electrophoresis

Cited by 2 publications

References 14 publications

What the Microscale Systems “See” In Biological Assemblies: Cells and Viruses?

What the Microscale Systems “See” In Biological Assemblies: Cells and Viruses?

CCR4 expressing cells cultured adherently on a capillary wall and formaldehyde fixed as the stationary phase for ligand screening by ACE

Contact Info

Product

Resources

About