Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation

Abstract

Bioremediation potential of bacteria and fungi isolated from sludge samples has been investigated (Danube alluvium, Pančevo, Serbia). Total isolated microorganisms were divided into three parts. One part was added with actidione antifungicide. The second part was added with streptomycin antibiotic. The third part was without additives. Paraffinic type of crude oil was a substrate for assessment of bioremediation potential. The simulated oil biodegradation lasted 30, 60 and 90 days. Parallel with that, the experiments with blind trial were conducted. Extracts were isolated from the samples with chloroform in a separate funnel. They were assayed for the group composition (alkanes, aromatics, alcohols and fatty acids) by column chromatography. Alkane fraction was analysed by gas chromatography-mass spectrometry (GC-MS). The most intense oil degradation was achieved in the experiments with bacteria, somewhat weaker with consortium of fungi and bacteria, and the weakest bioremediation potential in these experiments was shown by fungi.

Decolorization of adsorbed textile dyes by developed consortium of Pseudomonas sp. SUK1 and Aspergillus ochraceus NCIM-1146 under solid state fermentation

“…This suggests higher efficiency of the consortium-PA compared with individual microorganisms. Similar results were also reported in case of crude oil degradation using consortium of zymogenous bacteria and fungi [34].…”

Monitoring of underground water: Necessary step in determining the method for site remediation

“…Bioaugmentation implies adding microorganisms which are previously isolated form contamination sites and mulitiplied in bioreactors and returned to the site of contamination. The use of microbial populations characteristic for specific area prevents introduction of external pathogenes during the remediation process, which decreases the risk of disturbing the environmental equilibrium of the remediation site and the surrounding area [14,15,16,17]. The above-mentioned technology is successfully applied for treatment of water ecosystems contaminated with petroleum hydrocarbons.…”

Investigation of the bioremediation potential of aerobic zymogenous microorganisms in soil for crude oil biodegradation

“…The investigations performed under the laboratory conditions confirmed certain bioremediation potential of zymogenous microorganisms isolated from the waste water canal sludge for the degradation of oil. 11 Nevertheless, these microbial cultures showed lower potential for oil degradation than those isolated from the waters of the canal.…”

“…10,11 However, it must be stated that the biodegradation of crude oil by the soil aerobic zymogenous microorganisms at this location under natural conditions would be limited to the n-alkanes and isoprenoids. On the other hand, biodegradation of more complex polycyclic saturated and aromatic compounds, under the same conditions, would, most probably, not go to completion.…”

“…9 The possibility of using zymogenous microorganisms from the wastewater canal for bioremediation purposes has already been investigated in detail. 10,11 Based on an experiment of simulated biodegradation under laboratory conditions, it was proven that the dominant microorganisms from the surface water of the wastewater canal have degradable effects on petroleum hydrocarbons. 10 However, the biodegradation was restrained to the n-alkanes and isoprenoids, while polycyclic alkanes of sterane and triterpane type remained unchanged both in their abundance and distribution.…”