Investigation of Blood Flow Microcirculation by Diffusing Wave Spectroscopy

Meglinskii, I V; Korolevich, Alexander N.; Tuchin, Valery V.

doi:10.1615/critrevbiomedeng.v29.i3.150

Cited by 14 publications

(5 citation statements)

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“…The examples of these techniques include Laser Doppler Flowmetry (LDF) [12], Dynamic Light Scattering (DLS) [13], Diffusing Wave Spectroscopy (DWS) [14,15], Laser Speckle Contrast Analysis (LASCA) [16], Optical Coherence Tomography (OCT) [17], Doppler OCT [18,19], laser-scanning confocal microscopy [20], Spectrally Enhanced Microscopy (SEM) [21], Fluorescent Intravital Microscopy (FIM) [22] and other. While the techniques mentioned above are good for assessment of blood flow and vascular network structure as a stand-alone instruments, at the same time these modalities are quite limited for a comprehensive simultaneous observation of tumor microenvironment, tumor vascular networks and microcirculation monitoring in vivo.…”

Section: Biophotonicsmentioning

confidence: 99%

In vivo characterization of tumor and tumor vascular network using multi‐modal imaging approach

Kalchenko

Madar-Balakirski

Meglinski

et al. 2011

Journal of Biophotonics

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We present a multi-modal optical diagnostic approach utilizing a combined use of Fluorescence Intravital Microscopy (FIM), Dynamic Light Scattering (DLS) and Spectrally Enhanced Microscopy (SEM) modalities for in vivo imaging of tumor vascular network and blood microcirculation. FIM is used for imaging of tumor surroundings and microenvironment, SEM provides information regarding blood vessels topography, whereas DLS is applied for functional imaging of vascular network and blood microcirculation. This complementary combination of the imaging approaches is extremely useful for functional in vivo imaging of blood vasculature and tumor microenvironment. The technique has also a great potential in vascular biology and can significantly expand the capabilities of tumor angiogenesis studies and notably contribute to the development of cancer treatment.

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Section: Biophotonicsmentioning

confidence: 99%

In vivo characterization of tumor and tumor vascular network using multi‐modal imaging approach

Kalchenko

Madar-Balakirski

Meglinski

et al. 2011

Journal of Biophotonics

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“…Using the method of Doppler spectroscopy we investigated the speed of the perfusion of the blood in the skin 6 . The measurements were carried out using a PeriFlux 4001 from Perimed (Sweden).…”

Section: Experimental Peculiaritiesmentioning

confidence: 99%

Heating of blood by low-intensity laser radiation

et al. 2003

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Theoretical and experimental studies of the influence of low-intensity laser radiation, on the velocity of microcirculation of the erythrocytes of patients with the cardiovascular disease "in vivo" are carried out. Dynamic light scattering techniques were used to monitor the variation in the perfusion of micro capillary blood flow during irradiation under "in vivo" conditions and compared to the change in average size of aggregates of the blood effects observed "in vitro" using static scattering of light. It is shown that the process of the fragmentation of erythrocytes depends on amount of energy absorbed by biological tissues. This conclusion is supported by the good qualitative agreement with the theoretical model, based on the heat transfer theory within the dermis.

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“…Abnormalities of blood and/or lymph microcirculation are typically associated with diseases, including diabetes, arteriosclerosis, venous leg ulceration, anemia, and ischemia [2][3][4]. In the past a number of techniques have been applied for imaging and mon- itoring of micro-flows and micro-vessels functions, including magnetic resonance imaging [5], Doppler ultrasound [6], laser Doppler [7], optical coherence tomography (OCT) [8][9][10], Doppler OCT [11], laser speckle contrast analysis (LASCA) [12], capillaroscopy [13], orthogonal polarization spectral imaging (OPSI) [14], fluorescent intravital microscopy (FIM) [15], diffusing wave spectroscopy (DWS) [16,17], diffuse laser Doppler velocimetry (DLDV) [18], and laser-scanning confocal microscopy [19]. While the techniques mentioned above are very powerful for assessment of blood flow and vascular structure, they are quite limited to simultaneous observation of lymph and blood vessels.…”

Section: Introductionmentioning

confidence: 99%

Combined application of dynamic light scattering imaging and fluorescence intravital microscopy in vascular biology

et al. 2010

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The dynamic light scattering imaging (DLSI) system combined with the conventional fluorescence intravital microscope (FIM) has been applied for the examination of blood and lymph vessels in the mouse ear in vivo. While the CCD camera can be shared by both techniques the combined application of DLSI and FIM allows rapid switching between the modalities. In current study temporal speckles fluctuations are used for rendering blood vessels structure and monitoring blood perfusion with the higher spatial resolution, whereas FIM provides the images of lymphatic vessels. The results clearly demonstrate that combined application of DLSI and FIM approaches provides synchronic in vivo images of blood and lymph vessels with higher contrast and specificity. The use of this new dual-modal diagnostic system is particularly important and has a great potential to significantly expand the capabilities of vascular diagnostics providing synchronic in vivo images of blood and lymph vessels.Example of image (0.5×0.5 cm 2 ) of blood and lymph vessels in mouse ear obtained in vivo by combined use of DLSI and FIM

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Investigation of Blood Flow Microcirculation by Diffusing Wave Spectroscopy

Cited by 14 publications

References 0 publications

In vivo characterization of tumor and tumor vascular network using multi‐modal imaging approach

In vivo characterization of tumor and tumor vascular network using multi‐modal imaging approach

Heating of blood by low-intensity laser radiation

Combined application of dynamic light scattering imaging and fluorescence intravital microscopy in vascular biology

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