Investigation of Bufavirus and Parvovirus 4 in Patients with Gastro-Enteritis from the South-East of France

Simo-Fouda, Francis; Thirion, Laurence; Nougairède, Antoine; Luciani, Léa; Driouich, Jean-Sélim; Petit, Paul Rémi; Delaunay, Pascal; Charrel, Rémi N.

doi:10.3390/pathogens10091151

Cited by 3 publications

(1 citation statement)

References 35 publications

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“…BuV was first reported in the fecal samples from children with diarrhea in Burkina Faso in 2012 3 . Subsequently, BuV infections have been reported in diarrhea patients in several countries such as Bhutan, China, France, Finland, Netherlands, South Africa, Thailand and Turkey 4 – 11 . The occurrence of bufavirus infection in humans in those counties ranges from 0.3 to 4% 5 , 12 , 13 .…”

Section: Introductionmentioning

confidence: 99%

First detection and genetic characterization of canine bufavirus in domestic dogs, Thailand

Charoenkul,

Thaw,

Phyu

et al. 2024

Sci Rep

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Canine bufavirus (CBuV) was reported in domestic dogs worldwide. We conducted a survey of canine bufavirus in domestic dogs in Thailand from September 2016 to October 2022. Rectal swab samples (n = 531) were collected from asymptomatic dogs and dogs with gastroenteritis signs. The samples were tested for CBuV using PCR with specific primers to the VP1/VP2 gene, and 9.42% (50/531) was CBuV positive. Our findings showed that CBuVs could be detected in both symptomatic and healthy dogs. The Thai CBuVs were found in dogs from different age groups, with a significant presence in those under 1 year (12.60%) and dogs aged 1–5 years (7.34%) (p < 0.05), suggesting a high prevalence of Thai CBuVs in dogs under 5 years of age. We performed complete genome sequencing (n = 15) and partial VP1/VP2 sequencing (n = 5) of Thai CBuVs. Genetic and phylogenetic analyses showed that whole genomes of Thai CBuVs were closely related to Chinese and Italian CBuVs, suggesting the possible origin of Thai CBuVs. The analysis of VP1 and VP2 genes in Thai CBuVs showed that 18 of them were placed in subgroup A, while only 2 belonged to subgroup B. This study is the first to report the detection and genetic characterization of CBuVs in domestic dogs in Thailand. Additionally, surveillance and genetic characterization of CBuVs in domestic animals should be further investigated on a larger scale to elucidate the dynamic, evolution, and distribution of CBuVs.

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Section: Introductionmentioning

confidence: 99%

First detection and genetic characterization of canine bufavirus in domestic dogs, Thailand

Charoenkul,

Thaw,

Phyu

et al. 2024

Sci Rep

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Parvovirus B19V, Bocaviruses, and Other Human Parvoviruses

Söderlund‐Venermo

2023

ClinMicroNow

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Parvoviruses are small, nonenveloped icosahedral viruses with a linear single‐stranded‐DNA (ssDNA) genome in the family Parvoviridae in the realm Monodnaviria . The family Parvoviridae is divided into three subfamilies, Parvovirinae , Densovirinae , and the new Hamaparvovirinae , on the basis of their ability to infect vertebrates, invertebrates, or both, respectively, and their NS1 amino acid sequences. The first human parvoviruses identified were the nonpathogenic adeno‐associated viruses of the genus Dependoparvovirus , which are increasingly used in gene therapy. Human bocavirus (HBoV1) has the typical structure of a member of the Parvoviridae . The most common test for diagnosis of HBoV1 infection is DNA detection by qualitative PCR. Parvoviruses are unable to induce cells to divide, so they require actively dividing cells for their replication.

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Structural Characterization of Human Bufavirus 1: Receptor Binding and Endosomal pH-Induced Changes

Gulkis,

Luo,

Chipman

et al. 2024

Viruses

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Bufaviruses (BuV) are members of the Parvoviridae of the Protoparvovirus genus. They are non-enveloped, T = 1 icosahedral ssDNA viruses isolated from patients exhibiting acute diarrhea. The lack of treatment options and a limited understanding of their disease mechanisms require studying these viruses on a molecular and structural level. In the present study, we utilize glycan arrays and cell binding assays to demonstrate that BuV1 capsid binds terminal sialic acid (SIA) glycans. Furthermore, using cryo-electron microscopy (cryo-EM), SIA is shown to bind on the 2/5-fold wall of the capsid surface. Interestingly, the capsid residues stabilizing SIA binding are conserved in all human BuVs identified to date. Additionally, biophysical assays illustrate BuV1 capsid stabilization during endo–lysosomal (pH 7.4–pH 4) trafficking and capsid destabilization at pH 3 and less, which correspond to the pH of the stomach. Hence, we determined the cryo-EM structures of BuV1 capsids at pH 7.4, 4.0, and 2.6 to 2.8 Å, 3.2 Å, and 2.7 Å, respectively. These structures reveal capsid structural rearrangements during endo–lysosomal escape and provide a potential mechanism for this process. The structural insights gained from this study will add to the general knowledge of human pathogenic parvoviruses. Furthermore, the identification of the conserved SIA receptor binding site among BuVs provides a possible targetable surface-accessible pocket for the design of small molecules to be developed as anti-virals for these viruses.

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Investigation of Bufavirus and Parvovirus 4 in Patients with Gastro-Enteritis from the South-East of France

Cited by 3 publications

References 35 publications

First detection and genetic characterization of canine bufavirus in domestic dogs, Thailand

First detection and genetic characterization of canine bufavirus in domestic dogs, Thailand

Parvovirus B19V, Bocaviruses, and Other Human Parvoviruses

Structural Characterization of Human Bufavirus 1: Receptor Binding and Endosomal pH-Induced Changes

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