Investigation of Cannabidiol in the Mouse Drug Discrimination Paradigm

Mustafa, Mohammed Ahmed; Poklis, Justin L.; Karin, Kimberly N.; Elmer, Jayden A.; Porter, Joseph H.; Parra, Victoria Sandoval; Lu, Dai; Schlosburg, Joel E.; Lichtman, Aron H.

doi:10.1089/can.2022.0198

Cited by 3 publications

(2 citation statements)

References 53 publications

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“…Interestingly, a recent study demonstrated that the responsiveness of female rats to acute CBD depended on the stage of the estrous cycle, with female rats being responsive only in the late diestrus phase at a 10-fold lower dose than males, but females were unresponsive to acute CBD in the proestrus phase ( Hughes and Herron, 2019 ). Further, a bell-shaped dose–response relationship is usually reported for CBD effects on anxiety-like behavior ( Hughes and Herron, 2019 ; Mustafa et al, 2023 ), with anxiolytic effects of CBD being observed at lower doses (10 mg/kg) but less or no effects at higher doses ( Garcia-Baos et al, 2021 ; Xu et al, 2022 ). In the future, it would be interesting to monitor estrous cycle in female mice as well as use a range of CBD doses to get a better understanding of CBD’s effects on anxiety-like behavior in Tat transgenic mice.…”

Section: Discussionmentioning

confidence: 99%

Effects of acute cannabidiol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice

Yadav-Samudrala,

Gorman,

Barmada

et al. 2024

Front. Neurosci.

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BackgroundSome evidence suggests that cannabidiol (CBD) has potential to help alleviate HIV symptoms due to its antioxidant and anti-inflammatory properties. Here we examined acute CBD effects on various behaviors and the endocannabinoid system in HIV Tat transgenic mice.MethodsTat transgenic mice (female/male) were injected with CBD (3, 10, 30 mg/kg) and assessed for antinociception, activity, coordination, anxiety-like behavior, and recognition memory. Brains were taken to quantify endocannabinoids, cannabinoid receptors, and cannabinoid catabolic enzymes. Additionally, CBD and metabolite 7-hydroxy-CBD were quantified in the plasma and cortex.ResultsTat decreased supraspinal-related nociception and locomotion. CBD and sex had little to no effects on any of the behavioral measures. For the endocannabinoid system male sex was associated with elevated concentration of the proinflammatory metabolite arachidonic acid in various CNS regions, including the cerebellum that also showed higher FAAH expression levels for Tat(+) males. GPR55 expression levels in the striatum and cerebellum were higher for females compared to males. CBD metabolism was altered by sex and Tat expression.ConclusionFindings indicate that acute CBD effects are not altered by HIV Tat, and acute CBD has no to minimal effects on behavior and the endocannabinoid system.

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Section: Discussionmentioning

confidence: 99%

Effects of acute cannabidiol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice

Yadav-Samudrala,

Gorman,

Barmada

et al. 2024

Front. Neurosci.

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“…For the endocannabinoids and related lipids, the current study found no acute CBD effects and only a Tat effect for AA in which Tat expression upregulated AA levels in the striatum of male mice. Whereas previous preclinical studies have reported the lack of acute CBD at different doses to affect brain endocannabinoids and related lipids (despite high CBD concentrations in blood and whole brain [141]), multiple studies demonstrated changes in endocannabinoid levels and related lipids following Tat exposure [41,142,143]. Alterations of the endocannabinoid system in postmortem tissue of PLWH has been demonstrated previously with reported changes in CB1R and CB2R expression in the frontal lobe [144,145].…”

Section: Discussionmentioning

confidence: 87%

Effects of Acute Cannabidiol on Behavior and the Endocannabinoid System in HIV-1 Tat Transgenic Female and Male Mice

Yadav-Samudrala,

Gorman,

Barmada

et al. 2023

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(1) Background: Some evidence suggests that cannabidiol (CBD) has potential to help alleviate HIV symptoms due to its antioxidant and anti-inflammatory properties. Here we examined acute CBD effects on various behaviors and the endocannabinoid system in HIV Tat transgenic mice. (2) Methods: Tat transgenic mice (female/male) were injected with CBD (3,10,30 mg/kg) and assessed for antinociception, activity, coordination, anxiety, and recognition memory. Brains were taken to quantify endocannabinoids and related lipids. Additionally, CBD and metabolite 7-hydroxy-CBD were quantified in the plasma and cortex. (3) Results: Tat decreased striatal-related nociception and locomotion, with some sex-dependent effects on coordination. CBD had no effect on nociception and coordination, but increased locomotor activity. For anxiety, differential CBD effects were noted for sex, with decreasing anxiety-like behavior in males only. In the striatum and spinal cord, male sex was associated with lower 2-arachidonoylglycerol and with elevated concentrations of its proinflammatory metabolite arachidonic acid. CBD metabolism was altered by sex and Tat. (4) Conclusion: Findings indicate that acute CBD effects are not altered by HIV Tat, and CBD has minimal effects on behavior and the endocannabinoid system. Interestingly, sex-dependent alterations were noted for endocannabinoids and related lipids, which may be of relevance in view of potential CBD-based treatment options for people living with HIV.

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