Investigation of chemical composition and evaluation of antioxidant, antibacterial and antifungal activities of ethanol extract from Bidens pilosa L.

Abstract

Bidens pilosa L. is a species of plant that grows wild. It is commonly found in abundance in the Mekong Delta region of Vietnam. This study aimed to investigate the chemical composition, in vitro and in vivo antioxidant activity, the antimicrobial activity against some aquatic pathogenic bacteria and antifungal activity against plant pathogenic bacteria of Bidens pilosa L. extract. The results showed that Bidens pilosa L. extract had good antioxidant capacity through all four test methods of DPPH, ABTS •+ , RP, and TAC with the EC 50 values of 455.78 ± 3.28 μg/mL, 148.68 ± 2.02 μg/mL, 462.09 ± 12.57 μg/mL and 139.14 ± 4.34 μg/mL, respectively. Fruit flies fed on a diet supplemented with 0.5 g/mL of Bidens pilosa L. extract had an average lifespan of 2.15 times and 1.54 times longer under oxidative stress using 20 mM Paraquat and H 2 O 2 10%, respectively. In addition, the total polyphenols and total flavonoids in the extract were also determined to be 107.49 ± 4.04 mg GAE/g and 165.63 ± 2.90 mg QE/g, respectively.Regarding the antimicrobial activity, the ethanol extract of Bidens pilosa L. showed stronger resistance to Gram (+) S. agalactiae than the tested Gram (-) bacteria, including A. hydrophila, E. ictaluri, and A. dhakensis. In addition, the ethanol extract from Bidens pilosa L. also showed the better ability to inhibit the growth of the fungus Colletotrichum sp. (MIC = 1250 µg/mL) than that of Fusarium oxysporum (MIC = 2500 µg/mL).

Antioxidant activity and chemical composition of Spirolobium cambodianum Baill.

“…6 This suggested that the efficiency of activity might depend on geography and nutrition. Besides, comparison with the extract of several plants collected in Kien Giang province, such as Combretum quadrangulare, 16 Bidens pilosa, 18 Artemisia vulgaris, Pouzolzia zeylanica, and Costus speciosus, 19 S. cambodianum displayed the best antioxidant efficiency in tested assays. Therefore, the result demonstrated that SCE exerted its potent antioxidant activity due to the presence of active compounds.…”

“…Several reports about in vivo antioxidant activity of some plant extracts, such as Bidens pilosa L., Combretum quadrangulare on Drosophila melanogaster fly model, showed that under the same conditions of oxidative stress, with the addition of these plant extracts, the mean life of the flies will be faster than that of the flies supplemented with the extract of S. cambodianum. 16,18…”

In-vitro Evaluation of Wild Bitter Melon (Momordica charantia) Extracts against Periodontopathic Bacteria (Prevotella intermedia and Porphyromonas gingivalis) using Antibacterial, Anti-Inflammatory and Molecular Docking Analysis

“…The literature also highlights the World Health Organization’s emphasis on using natural substances instead of antibiotics derived from nature. [ 12 13 ] The pharmaceutical sector will then produce these substances to create natural antimicrobials with minimal adverse effects. [ 14 ]…”

Reactive eco-friendly dyeing of natural fabrics using a novel herbal composite containing extracts of Hemigraphis colorata and Bacopa monnieri

“…Hence, developing a novel antibacterial agents that has a dual function of preventing the process of drug resistance and improving the properties of antimicrobial textiles against infectious disease are highly essential. From different literature survey, the emphasis of World Health Organization on the replacing antibiotics with natural ingredients was noticed (Son et al, 2022). The organization instructs the researchers to use rich reservoirs of useful compounds from nature to replace chemical and synthetic antibacterial agents (Bulut et al, 2021).…”