Investigation of Chondrus crispus as a potential source of new antifouling agents

Chambers, Lily D.; Hellio, Claire; Stokes, K.R.; Dennington, Simon; Goodes, L.R.; Wood, R.J.K.; Walsh, Frank C.

doi:10.1016/j.ibiod.2011.07.002

Cited by 48 publications

(54 citation statements)

References 34 publications

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“…In addition, Chambers et al (2011) [23] reported that a crude ethanolic extract of dried C. crispus showed a greater AF effect than the extract derived from freshly harvested seaweed. However, the active components have yet to be identified, as the various NP extracts were not fully fractionated and assessed.…”

Section: Resultsmentioning

confidence: 99%

Anti-Biofilm Performance of Three Natural Products against Initial Bacterial Attachment

Salta

Wharton

Dennington

et al. 2013

IJMS

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Marine bacteria contribute significantly towards the fouling consortium, both directly (modern foul release coatings fail to prevent “slime” attachment) and indirectly (biofilms often excrete chemical cues that attract macrofouling settlement). This study assessed the natural product anti-biofilm performance of an extract of the seaweed, Chondrus crispus, and two isolated compounds from terrestrial sources, (+)-usnic acid and juglone, against two marine biofilm forming bacteria, Cobetia marina and Marinobacter hydrocarbonoclasticus. Bioassays were developed using quantitative imaging and fluorescent labelling to test the natural products over a range of concentrations against initial bacterial attachment. All natural products affected bacterial attachment; however, juglone demonstrated the best anti-biofilm performance against both bacterial species at a concentration range between 5–20 ppm. In addition, for the first time, a dose-dependent inhibition (hormetic) response was observed for natural products against marine biofilm forming bacteria.

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Section: Resultsmentioning

confidence: 99%

Anti-Biofilm Performance of Three Natural Products against Initial Bacterial Attachment

Salta

Wharton

Dennington

et al. 2013

IJMS

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“…Microalgal growth was assessed by analysis of liberated chlorophyll-a following centrifugation and addition of MeOH (100%). The released chlorophyll-a was quantified using a fluorometric method described by Chambers et al 60 The lowest concentration yielding a decrease in chlorophyll-a content was defined as the minimum inhibitory concentration (MIC) for algal growth. Microalgal adhesion was determined in a similar manner in which the non-attached algal cells were gently removed prior to addition of 100 µL of 100% methanol to release chlorophyll-a from the remaining algal biofilms.…”

Section: Methodsmentioning

confidence: 99%

Prevention of Marine Biofouling Using the Natural Allelopathic Compound Batatasin-III and Synthetic Analogues

Moodie¹,

Trépos

Cervin

et al. 2017

J. Nat. Prod.

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The current study reports the first comprehensive evaluation of a class of allelopathic terrestrial natural products as antifoulants in a marine setting. To investigate the antifouling potential of the natural dihydrostilbene scaffold, a library of 22 synthetic dihydrostilbenes with varying substitution patterns, many of which occur naturally in terrestrial plants, were prepared and assessed for their antifouling capacity. The compounds were evaluated in an extensive screen against 16 fouling marine organisms. The dihydrostilbene scaffold was shown to possess powerful general antifouling effects against both marine microfoulers and macrofoulers with inhibitory activities at low concentrations. The species of microalgae examined displayed a particular sensitivity toward the evaluated compounds at low ng/mL concentrations. It was shown that several of the natural and synthetic compounds exerted their repelling activities via nontoxic and reversible mechanisms. The activities of the most active compounds such as 3,5-dimethoxybibenzyl (5), 3,4-dimethoxybibenzyl (9), and 3-hydroxy-3′,4,5′-trimethoxybibenzyl (20) were comparable to the commercial antifouling booster biocide Sea-nine, which was employed as a positive control. The investigation of terrestrial allelopathic natural products to counter marine fouling represents a novel strategy for the design of “green” antifouling technologies, and these compounds offer a potential alternative to traditional biocidal antifoulants.

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“…Tenmarine bacterial strains from the bacterial collection of the University of Portsmouth School of Biological Sciences were used in the current study (Table 1). These selected strains are representative of fouling species in both estuarine and marine environments (Chambers et al 2011). They were grown at 26 °C in a marine medium, composed of 0.5 % peptone (neutralised bacteriological peptone, Oxoid LTD) in filtered (Whatman 1,001-270, pore size 11 μm) natural seawater from the Solent (UK).…”

Section: Marine Antibacterial Assaysmentioning

confidence: 99%

The Bromotyrosine Derivative Ianthelline Isolated from the Arctic Marine Sponge Stryphnus fortis Inhibits Marine Micro- and Macrobiofouling

et al. 2014

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The inhibition of marine biofouling by the bromotyrosine derivative ianthelline, isolated from the Arctic marine sponge Stryphnus fortis, is described. All major stages of the fouling process are investigated. The effect of ianthelline on adhesion and growth of marine bacteria and microalgae is tested to investigate its influence on the initial microfouling process comparing with the known marine antifoulant barettin as a reference. Macrofouling is studied via barnacle (Balanus improvisus) settlement assays and blue mussel (Mytilus edulis) phenoloxidase inhibition. Ianthelline is shown to inhibit both marine micro- and macrofoulers with a pronounced effect on marine bacteria (minimum inhibitory concentration (MIC) values 0.1-10 μg/mL) and barnacle larval settlement (IC50 = 3.0 μg/mL). Moderate effects are recorded on M. edulis (IC50 = 45.2 μg/mL) and microalgae, where growth is more affected than surface adhesion. The effect of ianthelline is also investigated against human pathogenic bacteria. Ianthelline displayed low micromolar MIC values against several bacterial strains, both Gram positive and Gram negative, down to 2.5 μg/mL. In summary, the effect of ianthelline on 20 different representative marine antifouling organisms and seven human pathogenic bacterial strains is presented.

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Investigation of Chondrus crispus as a potential source of new antifouling agents

Cited by 48 publications

References 34 publications

Anti-Biofilm Performance of Three Natural Products against Initial Bacterial Attachment

Anti-Biofilm Performance of Three Natural Products against Initial Bacterial Attachment

Prevention of Marine Biofouling Using the Natural Allelopathic Compound Batatasin-III and Synthetic Analogues

The Bromotyrosine Derivative Ianthelline Isolated from the Arctic Marine Sponge Stryphnus fortis Inhibits Marine Micro- and Macrobiofouling

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