Investigation of co‐electrospun gelatin:TiO₂/polycaprolactone:silk fibroin scaffolds for wound healing applications

Abstract

Electrospinning is a facile technique to create porous nanofiber scaffolds with a high capability of natural environment mimicking. Here, co‐electrospinning of gelatin:TiO2, polycaprolactone:silk fibroin (G:T/P:F) was investigated with the aim of wound healing. Polycaprolactone improved the mechanical properties and gelatin:TiO2 enhanced cellular interactions. Silk fibroin was extracted from the Bombyx mori silkworm and increased the cellular and mechanical properties of the designed scaffolds. The morphology of the scaffolds was observed with field emission scanning electron microscopy (FESEM) and confirmed bead free porous nanofibrous structure. The fiber diameter rose after silk fibroin and TiO2 addition but it was in the favorable fiber range. The contact angle, swelling, and mechanical properties of the G:T/P:F scaffolds supplied the requirements for skin tissue engineering. The designed scaffold showed over 80% cell viability with filopodia formation after 48 h according to cell adhesion observations and MTT assay results. The DAPI and LIVE/DEAD staining results confirmed high cell viability and the scratch assay observations proved the high wound healing potential of the G:T/P:F scaffolds. Furthermore, antibacterial properties were shown. It seems the designed scaffold can be useful in skin tissue regeneration. Further research may address the suggested skin scaffold.

Electrospun organic/inorganic hybrid nanofibers for accelerating wound healing: a review

“…100,101 Consequently, TiO 2 NPs find extensive application in wound treatment. 94,[102][103][104][105][106] Recently, Golipour et al 94 developed wound healing dressings by co-electrospinning of gelatin:TiO 2 and polycaprolactone:silk fibroin (G:T/P:F) (Fig. 5k).…”

“…(k) Co-electrospun gelatin:TiO 2 /PCL:silk fibroin scaffolds (G:T/P:F) for antisepsis; (l) the antibacterial activity of G/P:F and G:T/P:F scaffolds against S. aureus and E. coli. Reproduced with permission 94. Copyright 2022, Wiley Periodicals LLC.…”

Fabrication of Silk Fibroin‐Derived Fibrous Scaffold for Biomedical Frontiers

“…These findings suggested its effectiveness in skin tissue regeneration. [156] and c) SF nanofibers are coated on TiO 2 -NTs to regulate vancomycin release, antimicrobial characteristics, and improve bone integration. The plan illustrates the movement of vancomycin molecules across SF nanofibers.…”

Advances in wound dressing based on electrospinning nanofibers

“…Antimicrobial examination showed that PEO/CS (AgNPs) nanofibers had 51.2 ± 3.2 mm and 47.2 ± 2.1 mm inhibition circle diameters against E. coli and S. aureus , respectively (Figure 11b). Golipour et al 184 developed electrospinning nanofiber scaffolds using a blend of GE, TiO 2 , PLCL, and SF for applications in skin tissue regeneration and wound healing. The study highlighted that the inclusion of SF and TiO 2 in the nanofiber scaffolds significantly enhanced the hydrophobicity of PLCL, leading to a decrease in the contact angle from 86.5° to 54.6°.…”