Investigation of cryoprotectant thermophysical properties in the fast cooling cryopreservation by DSC technique

Amini, Mohammad; Benson, James D.

doi:10.1016/j.cryobiol.2022.11.110

Cited by 1 publication

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“…In fact, there are a number of engineering approaches to increase warming rates in ever larger tissues, including laser, microwave, and inductionbased warming [306][307][308][309]. However, in cryosolutions with low CPA concentrations under ultra-rapid cooling, it is plausible that ice recrystallization could be eliminated at a lower warming rate when only tiny ice crystals form in the cooling process [69].…”

Section: Discussionmentioning

confidence: 99%

“…However, by slow warming, de Graaf and Koster [68] reported low viability in rapidly frozen rat liver slices. By DSC technique, Amini and Benson [69] show that, under ultra-rapid freezing, ice recrystallization does not happen in low CPA concentrations during slow warming; however, in moderate and high CPA concentrations, ice recrystallization is a dominant part of ice formation in slow warming.…”

Section: Warmingmentioning

confidence: 99%

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Technologies for Vitrification Based Cryopreservation

Amini

Benson

2023

Bioengineering

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Cryopreservation is a unique and practical method to facilitate extended access to biological materials. Because of this, cryopreservation of cells, tissues, and organs is essential to modern medical science, including cancer cell therapy, tissue engineering, transplantation, reproductive technologies, and bio-banking. Among diverse cryopreservation methods, significant focus has been placed on vitrification due to low cost and reduced protocol time. However, several factors, including the intracellular ice formation that is suppressed in the conventional cryopreservation method, restrict the achievement of this method. To enhance the viability and functionality of biological samples after storage, a large number of cryoprotocols and cryodevices have been developed and studied. Recently, new technologies have been investigated by considering the physical and thermodynamic aspects of cryopreservation in heat and mass transfer. In this review, we first present an overview of the physiochemical aspects of freezing in cryopreservation. Secondly, we present and catalog classical and novel approaches that seek to capitalize on these physicochemical effects. We conclude with the perspective that interdisciplinary studies provide pieces of the cryopreservation puzzle to achieve sustainability in the biospecimen supply chain.

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