Investigation of Cryptosporidium spp. antigen by ELISA method in stool specimens obtained from patients with diarrhea

Elgun, Gullu; Koltas, Ismail Soner

doi:10.1007/s00436-010-2079-4

Cited by 34 publications

(24 citation statements)

References 4 publications

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“…This study also points out that frog samples sourced from Cryptosporidium oocysts as compared to flotation which agrees with previous reports [15].…”

Section: Discussionsupporting

confidence: 92%

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Occurrence of Cryptosporidium oocysts in edible frogs (Rana species) sold for human consumption in Hanwa frog market, Zaria, Kaduna State, Nigeria

Kia¹,

Ukuma²,

Odoba³

et al. 2017

JCLM

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Objective:To determine the occurrence of Cryptosporidium oocysts in edible frogs (Rana spp.) sold at the Hanwa frog market, Zaria, Kaduna State, Nigeria. Methods: A total of 117 frogs (Rana spp.) were randomly collected based on consent and availability at the market. The intestinal contents from the frogs were examined by staining flotation and sedimentation smears with modified Ziehl-Neelsen staining technique and microscopy of the oocysts. Results: Overall, 35.9% of frogs sampled from the Hanwa frog market were positive for Cryptosporidium oocysts. There were more Cryptosporidium oocysts detected by sedimentation test (28.2%) than flotation test (23.9%). There was no significant statistical association between sex of frogs and oocyst detection (χ 2 = 0.534 9, P > 0.05). Also, there was no correlation between the weights of frogs and the sizes of the Cryptosporidium oocysts detected (r = 0.010 9, P > 0.05). Nevertheless, female frogs (40%) and frogs within the weight range 170-219 g were more infected with Cryptosporidium (66.7%). Oocysts size range 6.10-7.00 µm had the highest frequency of 10 (23.8%). By size 28.2% of the oocysts detected suggested infection with Cryptosporidium parvum and Cryptosporidium maleagridis. Conclusions: This study has established that edible frogs (Rana spp.) sold at the Hanwa frog market for human consumption were infected with Cryptosporidium spp. which constitutes a valid public health risk especially for immunocompromised individuals.

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“…This study also points out that frog samples sourced from Cryptosporidium oocysts as compared to flotation which agrees with previous reports [15].…”

Section: Discussionsupporting

confidence: 92%

“…This poses a challenge for the water management system in the study area as pipe borne water will contain this organism which is highly resistant to chlorination of water, making the water unsafe for human consumption even after treatment of the water [15].…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Cryptosporidium oocysts in edible frogs (Rana species) sold for human consumption in Hanwa frog market, Zaria, Kaduna State, Nigeria

Kia¹,

Ukuma²,

Odoba³

et al. 2017

JCLM

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“…Similar findings were reported by El-Hamshary et al (2008), El-Settawy and Fathy (2012). However, Elgun and Koltas (2011) reported that ELISA had the highest score followed by PCR and AF stain, while Kehl et al (1995) ranked AF stain higher than ELISA.…”

Section: Discussionmentioning

confidence: 98%

“…Conflicting reports concerning the sensitivity of fecal immunoassays over microscopy were recorded (Kehl et al 1995). Different sensitivities of immunoassays for detection of Cryptosporidium coproantigen were reported (Morgan et al 1998;Abdel-Baki et al 2004;El-Hamshary et al 2008;Elgun and Koltas 2011;El-Settawy and Fathy 2012), possible explanation was that commercially available coproantigen detection formats react to different sets of surface epitopes using mAbs which may react weakly or may not react with antigens of different Cryptosporidium species in clinical samples. Added to this, microscopy and fecal immunoassays had a detection limit that had been reported to be 50,000-500,000 oocysts/g of stool (Smith 2008).…”

Section: Discussionmentioning

confidence: 99%

Laboratory utility of coproscopy, copro immunoassays and copro nPCR assay targeting Hsp90 gene for detection of Cryptosporidium in children, Cairo, Egypt

et al. 2014

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Cryptosporidium is a significant cause of diarrhea worldwide especially in children. Infection may end fatally in immunocompromised patients. Multi-attribute analysis was used to determine the lab utility of 4 diagnostics; coproscopy of AF stained fecal smear, fecal immunoassays by ICT and ELISA and copro-nPCR assay targeting Hsp90 gene, for detection of Cryptosporidium in stool of 250 Egyptian children (150 diarrheic and 100 nondiarrhaeic children). Also, to determine Cryptosporidium molecular prevalence. Cryptosporidium was an important enteric pathogen among both diarrheic and non-diarrheic study children with a clearly high prevalence of 16.4 % (n = 41). Conventional methods had perfect specificity (100 %) but couldn't be used as a consistent single detection method due to their lowered sensitivities. Multiattribute analysis ranked nPCR the highest test for lab use. Being the test with the best diagnostic yield, nPCR is a reliable diagnostic test and is going to replace conventional methods for reliable detection of Cryptosporidium.

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“…Araştırmalarda farklı yöntemler kullanılmış olsa da geri kalmış ülkelerde, 2 yaş altı çocuklarda [6][7][8], evcil hayvan besleyenlerde [9], gastroenteritlilerde [10,11], beslenme yetersizliklerinde [11), homoseksüellerde, immünyetersiz bireylerde, seyahat edenlerde, sıcak ve nemli ortam ve mevsimlerde enfeksiyonun daha sık görüldüğü bildirilmektedir [2]. Bu çalışmaların bazılarında Cryptosporidium ookistlerinin klorlanmış sularla veya herhangi bir işleme tabi tutulmayan sularla enfeksiyona neden olabileceği belirlenmiştir.…”

Section: Introductionunclassified

İshalli hastalarda direkt fluoresan antikor-dfa yöntemi ile giardia ve cryptosporidium spp. araştırılması

Özçelık¹,

Değerlı²,

Yıldırım³

2014

Cumhuriyet Med J

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ÖzetAmaç. Cryptosporidium spp. ve Giardia intestinalis insanda ishal nedeni olabilen önemli protozoonlardır. Bu parazitozların tanısında etkensel tanı yöntemlerinin yanında antijen tarama testleri de kullanılabilmektedir. Çalışmamızda ishalli hastalarda konvansiyonel yöntemlerle birlikte Direkt Fluoresan Antikor (DFA) yöntemi ile G.intestinalis ve Cryptosporidium spp. varlığının saptanması ve bu iki protozoonun gastroenterit olgularındaki rolünün araştırılması amaçlanmıştır. Yöntem. Sivas il merkezindeki üç hastanenin (Cumhuriyet Üniversitesi Uygulama ve Araştırma Hastanesi, Sivas Numune ve Sivas Devlet Hastanesi) çeşitli servislerine enterit yakınmalarıyla başvuran 32'si kadın, 68'i erkek toplam 100 ishalli hastaya ait dışkı örneklerinde direkt inceleme ve asit-fast boyama yöntemi ile yapılan mikroskobik incelemelerde parazitlere ait kist ve/veya ookistler, DFA yöntemi ile parazitlere ait antijenler araştırılmıştır. İncelenen grupta yer alan hastalar 0-80 yaş aralığında bulunmaktadır. Bulgular. Çalışmada, ishalli 100 dışkı örneğinin 2'sinde direkt mikroskobide G.intestinalis görülmüştür. Asit-fast boyama ile birinde Cryptosporidium spp. görülürken, DFA ile 4'ünde (%4,0) Cryptosporidium spp., 2'sinde (%2,0) G. intestinalis antijenlerine rastlanmıştır. Cryptosporidium spp. saptanan hastaların erişkin yaş grubunda yer alması ilginç bulunmuştur. Sonuç. Cryptosporidium spp. ve G. intestinalis'in ikisini de içeren DFA testleri rutin çalışmalarda ve prevalans araştırmalarında kullanılabilen kolay, duyarlı ve güvenilir bir testtir. Diğer taraftan enterit nedenleri arasında sayılan bu iki protozoon incelenen hasta grubunda toplam %6 oranında bulunmuştur.Anahtar sözcükler: Cryptosporidium spp., giardia intestinalis, DFA, ishal Abstract Aim. Cryptosporidium spp. and Giardia intestinalis are important parasitic protozoan causing diarrhea in developing and developed countries. Diagnosis of the Cryptosporidium oocyst in stool samples by conventional microscopy is labor-intensive and time-consuming. Thus, we aimed the presence of these protozoa in patients with diarrhea and to evaluate the usefulness of direct fluoresan antibody (DFA) test in detecting Cryptosporidium spp and G.intestinalis. from fecal specimens. Methods. For this aim, microscopy and specific antigen detection methods were used to determine Cryptosporidium spp. and G.intestinalis. One hundred stool specimens were examıned taken from patients with diarrhea whose ages ranged from 0 to 80 of applied to Hospital of Cumhuriyet University, Numune and Goverment Hospital in Sivas. All samples were tested for Cryptosporidium spp. G.intestinalis antigen by DFA and oocysts via gold Standard modified acidfast staining for Cryptosporidium spp.and G.intestinalis direct microscopy. Results. One specimen was found to be positive by modified acid-fast staining method and four specimens by DFA method were found to be positive for cryptosporidiosis. On the other hand two specimens were found to be positive both direct microscopy and DFA for giardiosis. Conclusion. The results o...

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Investigation of Cryptosporidium spp. antigen by ELISA method in stool specimens obtained from patients with diarrhea

Cited by 34 publications

References 4 publications

Occurrence of Cryptosporidium oocysts in edible frogs (Rana species) sold for human consumption in Hanwa frog market, Zaria, Kaduna State, Nigeria

Occurrence of Cryptosporidium oocysts in edible frogs (Rana species) sold for human consumption in Hanwa frog market, Zaria, Kaduna State, Nigeria

Laboratory utility of coproscopy, copro immunoassays and copro nPCR assay targeting Hsp90 gene for detection of Cryptosporidium in children, Cairo, Egypt

İshalli hastalarda direkt fluoresan antikor-dfa yöntemi ile giardia ve cryptosporidium spp. araştırılması

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