Investigation of Cytotoxic Effect of Black Mulberry (Morus nigra L.) Fruit

Dalkılıç, Semih; Dalkılıç, Lütfiye Kadıoğlu; İnci, Şule; Korkmaz, İsmail; Kırbağ, Sevda

doi:10.56042/ijtk.v20i1.29335

Cited by 4 publications

(1 citation statement)

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“…The MTT method operates on the principle that functional mitochondria within living cells reduce MTT, a tetrazolium salt capable of permeating the cell membrane. This reduction process involves the transfer of electrons within the cell, leading to the formation of water insoluble purple formazan crystals (Dalkilic et al, 2021;Dalkılıç et al, 2023b). When cells in 75 cm 2 flasks reached 90% confluence, the media was withdrawn, and the cells were cleaned with 5 mL of sterile Phosphate Buffered Saline (PBS).…”

Section: Determination Of Cytotoxic Activitymentioning

confidence: 99%

Investigation of Cytotoxic, Antimicrobial and Antioxidant Activity of Echium vulgare L. Seed

ARSLAN ATEŞŞAHİN,

KADIOĞLU DALKILIÇ,

ÖZEREN

et al. 2023

International Journal of Nature and Life Sciences

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Echium vulgare L. is a plant belonging to the Boraginaceae family and is used in traditional medicine for the treatment of various diseases, especially known for its anti-inflammatory and antioxidant properties. The aim of this study was to determine the cytotoxic activity of different concentrations of E. vulgare L. seed extracts obtained from methanol and hexane solvents on human breast cancer (MCF7) and human liver cancer (HepG2) cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The antioxidant activity of the extract obtained from methanol and hexane at the same concentrations was determined according to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity method. Antimicrobial activity was determined by disk diffusion method and bacteria such as Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli and Bacillus megaterium and Candida albicans microorganisms as fungi were used for the study. The highest antibacterial activity was detected against Escherichia coli (E. coli) with a zone diameter of 16±4 mm. Seed extract of E. vulgare prepared with hexane showed the most effective cytotoxic activity on HepG2 cell line with 20% cytotoxicity rate. In antioxidant activity tests, the methanol extract of E. vulgare exhibited the highest activity with 4.5% reduction. In conclusion, seed extracts of E. vulgare appear to be a potential source for both anticancer and antimicrobial therapies.

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Section: Determination Of Cytotoxic Activitymentioning

confidence: 99%

Investigation of Cytotoxic, Antimicrobial and Antioxidant Activity of Echium vulgare L. Seed

ARSLAN ATEŞŞAHİN,

KADIOĞLU DALKILIÇ,

ÖZEREN

et al. 2023

International Journal of Nature and Life Sciences

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Investigation of Cytotoxic Effects of Tragopogon reticulatus Against HepG2 and MCF-7 Cell Lines and Antioxidant, Antimicrobial Effects Against Some Microorganisms

Kadıoğlu Dalkılıç,

Dalkılıç

2024

OKÜ Fen Bil. Ens. Dergisi ((OKU Journal of Nat. & App. Sci)

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Plants have been used for various purposes throughout history. This subject has received increasing attention in the field of traditional medicine in recent years. Tragopogon reticulatus, as a forage grass plant in turkey, is a species that stands out with rich vitamin and mineral content. The aim of this study was to investigate the antimicrobial, antioxidant, and cytotoxic activities of T. reticulatus. Two separate extracts were prepared at different concentrations using hexane and methanol solvents obtained from the T. reticulatus plant, and cytotoxic activity was evaluated by the MTT Assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) method on human liver cancer (HepG2) and human breast cancer (MCF7) cell lines. The antioxidant activity of the extracts obtained from methanol and hexane was determined by the DPPH (2,2-diphenyl 1-picrylhydrazyl) radical scavenging capacity method. Antimicrobial activity was evaluated by the disk diffusion method, and bacteria such as Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Bacillus megaterium, and Candida albicans fungus were used in the study. The hexane extract obtained from the T. reticulatus plant showed a high cytotoxic activity of 44% in the MCF7 cell line, while it showed a 51% dead cell count in the HepG2 cell line. When the antioxidant results were evaluated, it was determined that the DPPH radical scavenging effect of the hexane extract exhibited the most effective activity at 19%. When the antimicrobial results were evaluated, it was determined that the concentrations prepared with hexane exhibited the most effective activity with a zone diameter of 16 mm against B. megaterium. These findings suggest that T. reticulatus plant extracts can be considered as a potential source. These results may shed light on future research into the biological effects of the plant and contribute to the development of new treatment methods.

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Investigation of phytochemical contents and anticancer, antioxidant, antimicrobial activities of Cucurbita pepo leaves

Saeed,

Temüz,

Keser

et al. 2024

Journal of Physical Chemistry and Functional Materials

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Ethanol and acetone extracts of Cucurbita pepo leaves were in vitro analyzed to determine the vitamin contents, antioxidant, anticancer, and antimicrobial activity of the leaves. According to HPLC analyses, the leaves contained slight amounts of vitamin C, A, and E. The analysis for antioxidant activity revealed that both ethanol and acetone extracts exhibited high activity values against ABTS+•, OH•, and DPPH• radicals. The total phenols and flavonoid assay revealed high values for the extracts. The MTT test revealed that both ethanol and acetone extracts exhibited cytotoxic activity toward human breast cancer cells (MCF-7). But no significant activities observed against hepatocellular carcinoma cells (HepG-2). The antimicrobial activity assay revealed activity of the extracts towards selected Gram's positive and Gram's negative bacteria. These results have provided scientific motivation to use Cucurbita pepo leaves as a medicinal plant to treat oxidative and inflammatory-related diseases.

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Investigation of Cytotoxic Effect of Black Mulberry (Morus nigra L.) Fruit

Cited by 4 publications

References 0 publications

Investigation of Cytotoxic, Antimicrobial and Antioxidant Activity of Echium vulgare L. Seed

Investigation of Cytotoxic, Antimicrobial and Antioxidant Activity of Echium vulgare L. Seed

Investigation of Cytotoxic Effects of Tragopogon reticulatus Against HepG2 and MCF-7 Cell Lines and Antioxidant, Antimicrobial Effects Against Some Microorganisms

Investigation of phytochemical contents and anticancer, antioxidant, antimicrobial activities of Cucurbita pepo leaves

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