Investigation of Differential Expression of Alpha-Gliadin Genes with Variable Immunogenic Epitopes in Hexa- and Tetraploid Wheat Cultivars Suggests Some Have Lower Immunogenicity

Arora, J. K.; Kaur, Simranjit; Yadav, Inderjit Singh; Kaur, Satinder; Chhuneja, Parveen

doi:10.1007/s11105-022-01356-4

Cited by 1 publication

(2 citation statements)

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“…Thereby, Juhász et al identified many proteins with strong immunoreactivity in the D subgenome and fewer in the B subgenome . In accordance, Arora et al found a lower expression of CD-active epitopes of α-gliadins in durum wheat than in common wheat, with genes on chromosome 6B showing the smallest expression . Differences in the genetic background of the species can also be detected by proteomic or peptidomics approaches.…”

Section: Introductionmentioning

confidence: 92%

“… 9 In accordance, Arora et al found a lower expression of CD-active epitopes of α-gliadins in durum wheat than in common wheat, with genes on chromosome 6B showing the smallest expression. 10 Differences in the genetic background of the species can also be detected by proteomic or peptidomics approaches. Prandi et al analyzed four common wheat cultivars, three durum wheat cultivars, and one cultivar of einkorn, emmer, and spelt, each concerning their peptide distribution after simulated human digestion.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Label-Free Proteomics Study on Celiac Disease-Active Epitopes in Common Wheat, Spelt, Durum Wheat, Emmer, and Einkorn

Norwig,

Geisslitz,

Scherf

2024

J. Agric. Food Chem.

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Wheat species with various ploidy levels may be different regarding their immunoreactive potential in celiac disease (CD), but a comprehensive comparison of peptide sequences with known epitopes is missing. Thus, we used an untargeted liquid chromatography tandem mass spectrometry method to analyze the content of peptides with CD-active epitope in the five wheat species common wheat, spelt, durum wheat, emmer, and einkorn. In total, 494 peptides with CD-active epitope were identified. Considering the average of the eight cultivars of each species, spelt contained the highest number of different peptides with CDactive epitope (193 ± 12, mean ± SD). Einkorn showed the smallest variability of peptides (63 ± 4) but higher amounts of certain peptides compared to the other species. The wheat species differ in the presence and distribution of CD-active epitopes; hence, the entirety of peptides with CD-active epitope is crucial for the assessment of their immunoreactive potential.

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Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%