Investigation of doublecortin and calcium/calmodulin‐dependent protein kinase‐like‐1‐expressing cells in the mouse stomach

Zhang, Yan; Huang, Xinlan

doi:10.1111/j.1440-1746.2009.06114.x

Cited by 12 publications

(14 citation statements)

References 29 publications

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“…The time courses of changes in the numbers of DCAMKL1 and PCNA cells 6 to 96 hours after ethanol treatment in rat are consistent with those in mouse[13]. In addition, we analyzed earlier changes in these cell types.…”

Section: Discussionsupporting

confidence: 58%

Altered expression of a putative progenitor cell marker DCAMKL1 in the rat gastric mucosa in regeneration, metaplasia and dysplasia

et al. 2010

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BackgroundDoublecortin and calcium/calmodulin-dependent protein kinase-like-1 (DCAMKL1) is a candidate marker for progenitor cells in the gastrointestinal mucosa. Lineage cells in the gastric mucosa are derived from progenitor cells, but this process can be altered after injury. Therefore, we explored DCAMKL1 expression under pathological conditions.MethodsAn immunohistochemical analysis was performed in rat stomach with acute superficial injury, chronic ulcer, intestinal metaplasia and dysplasia.ResultsDCAMKL1 was exclusively expressed in immature quiescent cells in the isthmus of normal fundic glands, where putative progenitor cells are thought to reside. DCAMKL1-positive cells and proliferating cells shed into the lumen after superficial injury and re-appeared during the regenerative process, mainly in the superficial mucosa. In the marginal mucosa around the active ulcer, parietal and chief cells diminished, foveolar hyperplasia was evident, and trefoil factor family 2 (TFF2)/spasmolytic polypeptide-expressing metaplasia (SPEM) emerged at the gland base. DCAMKL1 cells re-emerged in the deep mucosa juxtaposed with SPEM and proliferating cells. In the healing ulcer, the TFF2 cell population expanded and seemed to redifferentiate to chief cells, while proliferating cells and DCAMKL1 cells appeared above and below the TFF2 cells to promote healing. SPEM appeared and PCNA cells increased in the intestinalized mucosa, and DCAMKL1 was expressed in the proximity of the PCNA cells in the deep mucosa. DCAMKL1, PCNA and TFF2 were expressed in different dysplastic cells lining dilated glands near SPEM.ConclusionThe ultrastructural appearance of DCAMKL1-positive cells and the expression patterns of DCAMKL1 in normal and pathological states indicate that the cells belong to a progenitor cell population. DCAMKL1 expression is closely associated with TFF2/SPEM cells after injury. DCAMKL1 cells repopulate close to proliferating, hyperplastic, metaplastic and dysplastic cells, and the progenitor zone shifts according to the pathological circumstances.

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Section: Discussionsupporting

confidence: 58%

Altered expression of a putative progenitor cell marker DCAMKL1 in the rat gastric mucosa in regeneration, metaplasia and dysplasia

et al. 2010

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“…DCAMKL-1 has been evaluated in the stomach in two studies and was expressed in immature quiescent cells in the isthmus of normal gastric glands where proliferative cells and putative progenitor cells are thought to reside [31,32]. Lgr5 expression is identified in two to four cells at the base of the pyloric glands [33].…”

Section: Discussionmentioning

confidence: 99%

Murine Tissue-Engineered Stomach Demonstrates Epithelial Differentiation

Speer

Sala

Matthews

et al. 2011

Journal of Surgical Research

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“…Gastric DCLK1 cells express parietal cell markers and partially co-express MSI1. In rodent models of acute gastritis and of chronic ulcer DCLK1 cells expand beyond the stem cell zone but remain PCNA negative [33,34]. In these conditions as well as after irradiation, DCLK1 cells are found adjacent to the PCNA positive compartment, suggesting a possible role in the restitution of mucosal integrity [33,34].…”

Section: Dclk1 Expression In the Stomachmentioning

confidence: 98%

“…In the stomach, DCLK1 cells are found in the stem cell zone located at the isthmus of the fundic glands [33] and are clearly distinct from TFF2 transcript-expressing cells, which are considered the progenitor cells for mucus neck, parietal and zymogenic cells [12,34]. Gastric DCLK1 cells express parietal cell markers and partially co-express MSI1.…”

Section: Dclk1 Expression In the Stomachmentioning

confidence: 99%

DCLK1 expression in gastrointestinal stem cells and neoplasia

Bellows

Gagliardi

2012

J Cancer Ther Res

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Doublecortin-like kinase (Dclk1), a gene involved in neurogenesis, has recently been found expressed in single cells of the human and murine adult gastrointestinal tract. Currently, there are opposing opinions on the exact identity and functional characteristics of DCLK1 positive cells. Some view them as gastrointestinal stem cells that fuel the self-renewal process, whereas others view them as an intestinal brush cells or as an enteroendocrine subtype. Interestingly, Dclk1 is over-expressed in several gastrointestinal malignancies, but is expressed at only low levels in normal gastrointestinal cells, making it a possible target for cancer therapy. In view of the recent therapeutic efforts to antagonize and target the cancer stem cell population, there has been ongoing interest in DCLK1. In this review we critically appraise the studies describing the expression of Dclk1 in the normal and neoplastic gastrointestinal tract as well as discuss the possible function and of Dclk1 in the gastrointestinal system and the potential clinical relevance as a tumor marker and a therapeutic target.

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Investigation of doublecortin and calcium/calmodulin‐dependent protein kinase‐like‐1‐expressing cells in the mouse stomach

Abstract: Using DCAMKL-1 as a marker for stomach stem cells, we could describe the expression pattern of stomach stem cells during mucosal injury.

Cited by 12 publications

References 29 publications

Altered expression of a putative progenitor cell marker DCAMKL1 in the rat gastric mucosa in regeneration, metaplasia and dysplasia

Altered expression of a putative progenitor cell marker DCAMKL1 in the rat gastric mucosa in regeneration, metaplasia and dysplasia

Murine Tissue-Engineered Stomach Demonstrates Epithelial Differentiation

DCLK1 expression in gastrointestinal stem cells and neoplasia

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