Investigation of enrofloxacin residues in broiler tissues using ELISA and LC-MS/MS

Panzenhagen, Pedro; Aguiar, Waldemir Silva de; Gouvêa, Raquel; Oliveira, Andrea; Barreto, Fabiano; Pereira, Vla; Aquino, Maria Helena Cosendey de

doi:10.1080/19440049.2016.1143566

Cited by 18 publications

(17 citation statements)

References 19 publications

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“…The detection of ENR in edible tissues of broilers have also been evaluated in those reports (de Assis et al, 2016; Haag et al, 2016; Panzenhagen et al, 2016). Establishment of withdrawal periods are based on the depletion times of drugs, which allows the appropriate animal treatment and slaughter of treated animals, according to the requirement for the elimination and residues of drugs in animal tissues.…”

Section: Introductionmentioning

confidence: 99%

Clinical Efficacy and Residue Depletion of 10% Enrofloxacin Enteric-Coated Granules in Pigs

et al. 2017

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A new, more palatable formulation of 10% enrofloxacin enteric-coated granules was investigated to evaluate the pharmacokinetic effect in plasma, the residue elimination in tissues and the clinical efficacy against Actinobacillus pleuropneumonia (APP) and Mycoplasam suis (MS) in pigs. In this study, the enrofloxacin concentrations in plasma and tissues were detected using high-performance liquid chromatography with phosphate buffer (pH = 3) and acetonitrile. The pharmacokinetics and elimination of enrofloxacin enteric-coated granules were performed after oral administration at a single dose of 10 mg/kg body weight (bw) and 5 mg/kg twice per day for 5 consecutive days, respectively. The in vivo antibacterial efficacy and clinical effectiveness of enrofloxacin enteric-coated granules against APP and MS were assayed at 2.5, 5, 10 mg/kg, compared with tiamulin (8 mg/kg) based on establishment of APP and MS infection models. 56 APP strains were selected and tested for in vitro antibacterial activity of enrofloxacin enteric-coated granules. The main parameters of elimination half-life (t1/2β), Tmax, and area under the curve (AUC) were 14.99 ± 4.19, 3.99 ± 0.10, and 38.93 ± 1.52 μg h/ml, respectively, revealing that the enrofloxacin concentration remained high and with a sustainable distribution in plasma. Moreover, the analysis on the evaluation of enrofloxacin and ciprofloxacin in muscle, fat, liver and kidney showed that the recovery were more than 84% recovery in accordance with the veterinary drug residue guidelines of United States pharmacopeia, and the withdrawal periods were 4.28, 3.81, 4.84, and 3.51 days, respectively, suggesting that the withdrawal period was 5 d after oral administration of 5 mg/kg twice per day. The optimal dosage of enrofloxacin enteric-coated granules against APP and MS was 5 mg/kg, with over 90% efficacy, which was significantly different (p < 0.05) to the 2.5 mg/kg group, but not to the 10 mg/kg group or the positive control group (tiamulin). In conclusion, 10% enrofloxacin enteric-coated granules had significant potential for treating APP and MS, and it provided an alternative enrofloxacin palatability formulation.

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Section: Introductionmentioning

confidence: 99%

Clinical Efficacy and Residue Depletion of 10% Enrofloxacin Enteric-Coated Granules in Pigs

et al. 2017

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“…The highest levels of resistance were observed in abattoir 3 (Table 2), a commercial abattoir, located at metropolitan region of Rio de Janeiro In Brazil, enrofloxacin is frequently employed in poultry breeding, and there is no effective control by regulatory agencies of its use in small producers, as may be verified in the report of the State Program for the Control of Veterinary Medicine Wastes in Animal-derived Food (PAMvet) in the state of Paraná, Brazil (Machinski Junior et al 2005). In fact, Panzenhagen et al (2016b) registered enrofloxacin wastes in 72.2% of broilers' samples collected in the state of Rio de Janeiro, analyzed by Enzyme-Linked Immunosorbent Assay (ELISA), albeit at rates lower than the Maximum Waste Limits. In Spain, Cantero et al (2018) reported that (Lin et al 2002) isolated strains of broilers were predominantly resistant to quinolones, perhaps due to the frequent use of enrofloxacin in poultry breeding.…”

Section: Resultsmentioning

confidence: 99%

Detection of efflux pump CmeABC in enrofloxacin resistant Campylobacter spp. strains isolated from broiler chickens (Gallus gallus domesticus) in the state of Rio de Janeiro, Brazil

et al. 2019

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Fowls are the main reservoirs of the highly important food-originating pathogen called Campylobacter spp. and broilers’ meat and byproducts are the main vehicles of this microorganism. Increasing of Campylobacter spp. resistant strains to fluorquinolones, an antimicrobial class often employed in poultry farming and in human medicine has become a great concern to poultry breeders. In fact, several studies evaluated increasing bacterial resistance against these antimicrobial agents. The role of CmeABC efflux system has been underscored among the resistance mechanisms in Campylobacter spp. to fluorquinolones. This study investigated the occurrence of CmeABC efflux pump in 81 and 78 enrofloxacin resistant strains of Campylobacter jejuni and C. coli respectively, isolated from broilers collected from six abattoirs situated at São José do Vale do Rio Preto/RJ poultry center and from two commercial abattoirs situated at Metropolitan Region of Rio de Janeiro, from 2013 to 2016. The resistance to enrofloxacin was assessed by agar dilution to determine minimum inhibitory concentration (MIC). The CmeABC efflux system was investigated through the detection of genes genes cmeA, cmeB and cmeC by PCR. The activity of CmeABC efflux pump was investigated in 20 strains by using the efflux pump inhibitor Phenylalanine-Arginine β-Naphthylamide (PAβN). The three genes cmeA, cmeB and cmeC were detected in 94.3% of the strains (C. jejuni = 80 and C. coli = 70), whereas the system was absent or incomplete in 5.7% of strains (C. jejuni = 1 and C. coli = 8). MIC varied between 0.5μg/ml and 64μg/ml, and 88.7% of strains were enrofloxacin resistant and 11.3% featuring intermediate resistance. The inhibition of the efflux pump by PAβN reduced the MIC to enrofloxacin up to eight times in fifteen strains (75%). These results indicate that this system is frequent and active in Campylobacter spp. Resistant strains in the presence of enrofloxacin.

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“…Quinolones are powerful options among the antimicrobial agents employed in the treatment of various bacterial-caused poultry diseases (29). In addition, the presence of quinolones in poultry products causes public health concerns (21). Local and international regulatory and public health agencies give attention to screening of antibiotic residues in foodproducing animals.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Fluoroquinolones, essential drugs for human and animal health, are widely used for the treatment of several diseases in veterinary medicine (21,24). Enrofloxacin (ENR), a common fluoroquinolone, has a broad spectrum of action and exhibits high efficacy against infectious diseases that are caused by mycoplasma and gram-positive and gram-negative bacteria (14,21). Furthermore, this antibiotic is used in the treatment of common poultry infections, such as mycoplasmal infections, colibacillosis and pasteurellosis, due to its rapid oral absorption and long elimination half-life (10,22).…”

Section: Introductionmentioning

confidence: 99%

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Investigation of enrofloxacin and ciprofloxacin residues by LC-MS/MS in chicken liver marketed in Afyonkarahisar

Acaröz

Sözbilir

2020

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Antibiotic residues can remain in animal source foods, mainly in the liver. Enrofloxacin (ENR) is a synthetic fluoroquinolone antimicrobial agent which is extensively used to treat bacterial infections in poultry. This study aimed to evaluate ENR and its main metabolite ciprofloxacin (CIP) occurrence in 100 chicken livers marketed Afyonkarahisar, Turkey. Chicken liver samples were analyzed by LC-MS/MS method with limits of detection for 1.17 μg/kg (ENR) and 1.24 μg/kg (CIP). ENR and CIP were detected in 39% and in 31% of samples, respectively. However, the total sum of both antibiotic residue levels was found to be far below the established maximum residue level (200 μg/kg) in all of chicken liver samples. In conclusion, according to the results of this study, chicken livers marketed in Afyonkarahisar city are safe for public health regarding ENR and CIP residues. Nonetheless, periodical control of chicken liver is advised due to the high incidence of these antibiotics.

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Investigation of enrofloxacin residues in broiler tissues using ELISA and LC-MS/MS

Cited by 18 publications

References 19 publications

Clinical Efficacy and Residue Depletion of 10% Enrofloxacin Enteric-Coated Granules in Pigs

Clinical Efficacy and Residue Depletion of 10% Enrofloxacin Enteric-Coated Granules in Pigs

Detection of efflux pump CmeABC in enrofloxacin resistant Campylobacter spp. strains isolated from broiler chickens (Gallus gallus domesticus) in the state of Rio de Janeiro, Brazil

Investigation of enrofloxacin and ciprofloxacin residues by LC-MS/MS in chicken liver marketed in Afyonkarahisar

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