Investigation of essential cell cycle regulator genes as candidates for immortalized shrimp cell line establishment based on the effect of in vitro culturing on gene expression of shrimp primary cells

Thammasorn, Thitiporn; Nozaki, Reiko; Kondo, Hidehiro; Hirono, Ikuo

doi:10.1016/j.aquaculture.2020.735733

“…Lessons may be drawn from the failure to sustain in vitro the neoplastic hemocyte proliferation observed in vivo in spontaneously occurring clam leukemia, with research pointing to a role for the stress protein mortalin in the induction of apoptosis in cancerous hemocytes (Walker et al 2013). The RNA-seq approach may be applied to compare gene expression patterns in cultured cells and initial tissue, with a focus on essential cell proliferation and cell cycle arrest regulator genes, in order to develop future strategies for immortalization, as recently explored for developing shrimp cell lines (Thammasorn et al 2020).…”

Section: Perspectivesmentioning

confidence: 99%

From Primary Cell and Tissue Cultures to Aquatic Invertebrate Cell Lines: An Updated Overview

Domart‐Coulon¹,

Blanchoud²

2022

Advances in Aquatic Invertebrate Stem Cell Research

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He is a member of the editorial board of the Invertebrate Survival Journal and the European Journal of Zoology, and is a founding member of the Italian Association of Developmental and Comparative Immunobiology (IADCI). His main research interests are the evolution of innate immunity and the study of the cellular and molecular basis of immune responses in marine invertebrates, with particular reference to the role of hemocytes/coelomocytes in immune defense and, more generally, in the stress response. Most of his studies were carried out using the colonial ascidian Botryllus schlosseri as a model organism. His interest in stem cells is directly related to their role in hematopoiesis, as they assure the continuous renewal of the circulating immunocytes of marine invertebrates. He is the chair of the COST Action 16203 MARISTEM "Stem cells of marine/aquatic invertebrates: from basic research to innovative applications" that supported the publication of this book. He is the author or co-author of more than 130 peer-reviewed publications in scientific journals, co-editor of scientific books, including "Lessons in immunity: from singlecell organisms to mammals" (Elsevier, 2016), and guest editor of Special Issues, including: "Ancient immunity. phylogenetic emergence of recognition-defence mechanisms" (Biology (Basel), 2020-2021).Baruch (Buki) Rinkevich has been a professor and senior scientist at the

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“…Lessons may be drawn from the failure to sustain in vitro the neoplastic hemocyte proliferation observed in vivo in spontaneously occurring clam leukemia, with research pointing to a role for the stress protein mortalin in the induction of apoptosis in cancerous hemocytes (Walker et al 2013). The RNA-seq approach may be applied to compare gene expression patterns in cultured cells and initial tissue, with a focus on essential cell proliferation and cell cycle arrest regulator genes, in order to develop future strategies for immortalization, as recently explored for developing shrimp cell lines (Thammasorn et al 2020).…”

Section: Perspectivesmentioning

confidence: 99%

Appendix B, Table A2 From Primary Cell and Tissue Cultures to Aquatic Invertebrate Cell Lines: An Updated Overview

Domart‐Coulon¹,

Blanchoud²

2022

Advances in Aquatic Invertebrate Stem Cell Research

0

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He is a member of the editorial board of the Invertebrate Survival Journal and the European Journal of Zoology, and is a founding member of the Italian Association of Developmental and Comparative Immunobiology (IADCI). His main research interests are the evolution of innate immunity and the study of the cellular and molecular basis of immune responses in marine invertebrates, with particular reference to the role of hemocytes/coelomocytes in immune defense and, more generally, in the stress response. Most of his studies were carried out using the colonial ascidian Botryllus schlosseri as a model organism. His interest in stem cells is directly related to their role in hematopoiesis, as they assure the continuous renewal of the circulating immunocytes of marine invertebrates. He is the chair of the COST Action 16203 MARISTEM "Stem cells of marine/aquatic invertebrates: from basic research to innovative applications" that supported the publication of this book. He is the author or co-author of more than 130 peer-reviewed publications in scientific journals, co-editor of scientific books, including "Lessons in immunity: from singlecell organisms to mammals" (Elsevier, 2016), and guest editor of Special Issues, including: "Ancient immunity. phylogenetic emergence of recognition-defence mechanisms" (Biology (Basel), 2020-2021).Baruch (Buki) Rinkevich has been a professor and senior scientist at the

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“…This represents a serious obstacle to the full elucidation of the stem cell and immune cell properties in this group of animals and to promote their application in various disciplines ( 267 , 268 ). The main limitations in cell harvesting lie in the in vitro requirements which leads to the failure of most invertebrate primary cultures within 24-72 h post cell isolation ( 7 , 267 ), a topic that has been recently tested from an experimental point of view ( 269 ). However, some progress has been made as reported below.…”

Section: Stem and Immune Cells Open Queries New Tools And Future Perspectivesmentioning

confidence: 99%

Stem Cells and Innate Immunity in Aquatic Invertebrates: Bridging Two Seemingly Disparate Disciplines for New Discoveries in Biology

Ballarin

¹

,

Karahan

²

,

Salvetti

³

et al. 2021

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The scopes related to the interplay between stem cells and the immune system are broad and range from the basic understanding of organism’s physiology and ecology to translational studies, further contributing to (eco)toxicology, biotechnology, and medicine as well as regulatory and ethical aspects. Stem cells originate immune cells through hematopoiesis, and the interplay between the two cell types is required in processes like regeneration. In addition, stem and immune cell anomalies directly affect the organism’s functions, its ability to cope with environmental changes and, indirectly, its role in ecosystem services. However, stem cells and immune cells continue to be considered parts of two branches of biological research with few interconnections between them. This review aims to bridge these two seemingly disparate disciplines towards much more integrative and transformative approaches with examples deriving mainly from aquatic invertebrates. We discuss the current understanding of cross-disciplinary collaborative and emerging issues, raising novel hypotheses and comments. We also discuss the problems and perspectives of the two disciplines and how to integrate their conceptual frameworks to address basic equations in biology in a new, innovative way.

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Investigation of essential cell cycle regulator genes as candidates for immortalized shrimp cell line establishment based on the effect of in vitro culturing on gene expression of shrimp primary cells

Cited by 13 publications

References 68 publications

<p>From Primary Cell and Tissue Cultures to Aquatic Invertebrate Cell Lines: An Updated Overview</p> <p> </p>

<p>From Primary Cell and Tissue Cultures to Aquatic Invertebrate Cell Lines: An Updated Overview</p> <p> </p>

<p>Appendix B, Table A2<br />From Primary Cell and Tissue Cultures to Aquatic Invertebrate Cell Lines: An Updated Overview</p> <p> </p>

Stem Cells and Innate Immunity in Aquatic Invertebrates: Bridging Two Seemingly Disparate Disciplines for New Discoveries in Biology

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Investigation of essential cell cycle regulator genes as candidates for immortalized shrimp cell line establishment based on the effect of in vitro culturing on gene expression of shrimp primary cells

Cited by 13 publications

References 68 publications

<p>From Primary Cell and Tissue Cultures to Aquatic Invertebrate Cell Lines: An Updated Overview</p> <p>&nbsp;</p>

<p>From Primary Cell and Tissue Cultures to Aquatic Invertebrate Cell Lines: An Updated Overview</p> <p>&nbsp;</p>

<p>Appendix B, Table A2<br />From Primary Cell and Tissue Cultures to Aquatic Invertebrate Cell Lines: An Updated Overview</p> <p>&nbsp;</p>

Stem Cells and Innate Immunity in Aquatic Invertebrates: Bridging Two Seemingly Disparate Disciplines for New Discoveries in Biology

Contact Info

Product

Resources

About

<p>From Primary Cell and Tissue Cultures to Aquatic Invertebrate Cell Lines: An Updated Overview</p> <p> </p>

<p>From Primary Cell and Tissue Cultures to Aquatic Invertebrate Cell Lines: An Updated Overview</p> <p> </p>

<p>Appendix B, Table A2<br />From Primary Cell and Tissue Cultures to Aquatic Invertebrate Cell Lines: An Updated Overview</p> <p> </p>