Investigation of feather follicle morphogenesis and the expression of the Wnt/β-catenin signaling pathway in yellow-feathered broiler chick embryos

Xie, Wenyan; Chen, M J; Jiang, Shiguang; Yan, Huichao; Wang, X Q; Gao, Chun‐qi

doi:10.1080/00071668.2020.1758302

Cited by 14 publications

(13 citation statements)

References 42 publications

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“…Prior research has shown that between the ages of E12 and E14, goose skin begins to develop short feather buds [ 3 , 15 , 23 ], but in our study, histomorphological observations of the Zhedong white goose embryonic skin revealed that the formation of short feather buds appeared at E13, proposing that feather bud initiation may be linked to variations in goose breeds or hatching conditions. Additionally, the outcomes of this study demonstrated that feather follicle morphogenesis was finished by E18, which was congruent with earlier findings in chickens at E15 [ 24 ]. Thus, it was hypothesized that feather follicles form earlier in the chick embryo than in the goose, presumably due to the longer embryonic development period in geese (31 d) compared to chickens (21 d).…”

Section: Discussionsupporting

confidence: 92%

Transcriptional Characteristics Showed That miR-144-y/FOXO3 Participates in Embryonic Skin and Feather Follicle Development in Zhedong White Goose

Mabrouk

Zhou

Wang

et al. 2022

Animals

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Skin and feather follicle development are essential processes for goose embryonic growth. Transcriptome and next-generation sequencing (NGS) network analyses were performed to improve the genome of Zhedong White goose and discover the critical genes, miRNAs, and pathways involved in goose skin and feather follicle morphogenesis. Sequencing output generated 6,002,591,668 to 8,675,720,319 clean reads from fifteen libraries. There were 1234, 3024, 4416, and 5326 different genes showing differential expression in four stages, E10 vs. E13, E10 vs. E18, E10 vs. E23, and E10 vs. E28, respectively. The differentially expressed genes (DEGs) were found to be implicated in multiple biological processes and pathways associated with feather growth and development, such as the Wnt signaling pathway, cell adhesion molecules, ECM–receptor interaction signaling pathways, and cell cycle and DNA replication pathways, according to functional analysis. In total, 8276 DEGs were assembled into twenty gene profiles with diverse expression patterns. The reliability of transcriptome results was verified by real-time quantitative PCR by selecting seven DEGs and five miRNAs. The localization of forkhead box O3 (FOXO3), connective tissue growth factor (CTGF), protein parched homolog1 (PTCH1), and miR-144-y by in situ hybridization showed spatial-temporal expression patterns and that FOXO3 and miR-144-y have an antagonistic targeting relationship. The correlation coefficient of FOXO3 and miR-144-y was -0.948, showing a strong negative correlation. Dual-luciferase reporter assay results demonstrated that miR-144-y could bind to the expected location to suppress the expression of FOXO3, which supports that there is a targeting relationship between them. The detections in this report will provide critical insight into the complex molecular mechanisms and breeding practices underlying the developmental characteristics of skin and feather follicles in Zhedong white geese.

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Section: Discussionsupporting

confidence: 92%

Transcriptional Characteristics Showed That miR-144-y/FOXO3 Participates in Embryonic Skin and Feather Follicle Development in Zhedong White Goose

Mabrouk

Zhou

Wang

et al. 2022

Animals

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“…The skin color, uniformity, and color consistency of poultry were important reference attributes for consumers to choose and buy. Currently, research on the carcass characteristics of yellow-feathered broilers is mainly limited to skin hair follicles [ 16 , 32 ]. Therefore, three parameters related to the slaughter characteristics of yellow-feathered broilers were examined, including the follicle density, the skin color, and the spotted skin proportions.…”

Section: Resultsmentioning

confidence: 99%

Indigenous, Yellow-Feathered Chickens Body Measurements, Carcass Traits, and Meat Quality Depending on Marketable Age

Yuan

Jiang

Wang

et al. 2022

Animals

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Given an increasing trend in slaughter and chilling for the sale of chickens in China, it is important to determine the marketable age of chickens for chilled sales. This study determined the effects of two marketable ages on the body measurements, carcass traits, and meat quality of yellow-feathered chickens. A total of 360 healthy one-day-old male Xueshan chickens were raised in six pens (straw-covered floor, numbered 1 to 6) and treated in the same manner (free access to food and water) until day 100. Sixty chickens from pens numbered 1 to 3 and 4 to 6 were selected to determine the body measurements, carcass traits, and meat quality at two slaughter ages (90 and 100 days), respectively. One hundred-day-old chickens had a higher body slope, cockscomb, keel, shank lengths, and higher live and dressed weights (p < 0.05). The abdomen skin follicle density, a*(redness) and b*(yellowness) values were higher in 100-day-old chickens (p < 0.05), whereas the 90-day-old chickens were characterized by better spotted skin. For the breast muscle, pH, shear force, a*, moisture, and protein and intramuscular fat contents were lower; moreover, L*(lightness) and b* were higher in 90-day-old chickens. In leg muscles, the pH, shear force, L*, b* and collagen content were lower; furthermore, the a* and moisture contents were higher in 90-day-old chickens (p < 0.05). These findings indicate that two marketable ages both have pros and cons, but 90 days chickens perform better on carcass appearance, and producers can adjust the marketable age to meet needs of different consumers. This study provides a unique idea and theoretical reference for breeding and marketing yellow-feathered chickens.

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“…The previous studies have suggested that there are a lot of DEGs in geese dorsal tissues across embryonic days and in different breeds ( Liu, et al, 2020 ; Liu, et al, 2018 ; Lowe, et al, 2015 ; Sello, et al, 2019 ) and these DEGs participate in the Wnt, BMP, MAPK, and Notch pathways. With the advancement of genetic engineering techniques, such as gene knockout and gene location mutation, it has been confirmed that the Wnt signaling pathway plays a key regulatory role during feather follicle development ( Andl, et al, 2002 ; Liu, et al, 2020 ; Xie, et al, 2020a ). The Wnt signaling pathway and its effector β-catenin have highly dynamic regulation effects on the developmental processes of feather follicles at each stage, especially at the early feather follicle morphogenesis stage during the embryonic period.…”

Section: Discussionmentioning

confidence: 99%

In Ovo Injection of CHIR-99021 Promotes Feather Follicle Development via Modulating the Wnt Signaling Pathway and Transcriptome in Goose Embryos (Anser cygnoides)

Feng

Gong

et al. 2022

Front. Physiol.

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Feather performs important physiological functions in birds, and it is also one of the economic productions in goose farming. Understanding and modulating feather follicle development during embryogenesis are essential for bird biology and the poultry industry. CHIR-99021 is a potent Wnt/β-catenin signaling pathway activator associated with feather follicle development. In this study, goose embryos (Anser cygnoides) received an in ovo injection of CHIR-9902, which was conducted at the beginning of feather follicle development (E9). The results showed that feather growth and feather follicle development were promoted. The Wnt signaling pathway was activated by the inhibition of GSK-3β. Transcriptomic analyses showed that the transcription changes were related to translation, metabolism, energy transport, and stress in dorsal tissue of embryos that received CHIR-99021, which might be to adapt and coordinate the promoting effects of CHIR-99021 on feather follicle development. This study suggests that in ovo injection of CHIR-99021 is a potential strategy to improve feather follicle development and feather-related traits for goose farming and provides profiling of the Wnt signaling pathway and transcriptome in dorsal tissue of goose embryos for further understanding of feather follicle development.

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Investigation of feather follicle morphogenesis and the expression of the Wnt/β-catenin signaling pathway in yellow-feathered broiler chick embryos

Cited by 14 publications

References 42 publications

Transcriptional Characteristics Showed That miR-144-y/FOXO3 Participates in Embryonic Skin and Feather Follicle Development in Zhedong White Goose

Transcriptional Characteristics Showed That miR-144-y/FOXO3 Participates in Embryonic Skin and Feather Follicle Development in Zhedong White Goose

Indigenous, Yellow-Feathered Chickens Body Measurements, Carcass Traits, and Meat Quality Depending on Marketable Age

In Ovo Injection of CHIR-99021 Promotes Feather Follicle Development via Modulating the Wnt Signaling Pathway and Transcriptome in Goose Embryos (Anser cygnoides)

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