Investigation of Fecal Microbial Community Structure Using 16s rDNA Sequence in Pigs Fed Fermented Liquid Feed

Kakugawa, Koji; Ijiri, Satoru; Nakano, Kimitaka; Yamauchi, Shinya; Tsuchiya, Yoshinobu

doi:10.5938/youton.44.40

Cited by 1 publication

(1 citation statement)

References 18 publications

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“…DNA samples were used as a template to amplify fragments of the 16S rRNA gene with the universal primers 341F-GC (5'-CGCCCGCCGCGCCCCGCGCCC GTCCCGCCGCCCCGCCCGCCTACGGGAGGCAGCAG-3') and 907R (5'-CCGTCAATTCMTTTRAGTTT-3') [11]. PCR reaction mixture (20 μL) contained 0.25-µmol amounts of each primer, 2.5mM concentrations of each deoxyribonucleotide triphosphate, 2μL10×buffer, 20mM tetramethylammonium chloride, 0.1μL of TaKaRa EX Taq (Takara Bio Inc.), and 1 μl of DNA solution.…”

Section: Profile Analysis Of Cecal Microbiota By Pcr-denaturing Gradimentioning

confidence: 99%

Plasma Concentrations of GLP-1 and PYY in Rats Fed Dietary Fiber Depend on the Fermentability of Dietary Fiber and Respond to an Altered Diet

Shimada¹,

Yoshimura²,

Murakami³

et al. 2015

Int J Clin Nutr Diet

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Background: It was reported to be associated with intake of dietary fiber and gut hormone. We compared the effect of dietary fiber on the plasma concentration of glucagon-like peptide-1 (GLP-1) and peptide YY (PYY). Methods: Firstly, male Wistar rats (10 wk old) were fed a fiber-free diet (FF diet, AIN-93G-based diet without cellulose) or one of nine FF diets with 5% dietary fiber for 21 days. Cellulose, guar gam (GG), partially hydrolyzed guar gum (PHGG), sugar beet fiber (BF), konjac mannnan (KM), raffinose (RF), hydroxypropyl starch (HPS), hydroxypropyl distarch phosphate (HPDP) and Akamoku (AK) were used as dietary fiber sources. Secondly, we then determined whether the concentrations of GLP-1 and PYY in portal and aorta plasma and microbiota in cecal contents responded to an altered diet. Male Wistar rats (10 wk old) were fed the PHGG diet for 20 days before being changed to the FF diet. Results: The amount of short chain fatty acids (SCFA) in the cecal contents markedly increased in rats fed the GG, PHGG, BF, KM, RF, HPS or HPDP diets compared with SCFA in rats fed the FF, CL or AK diets. The concentrations of GLP-1 and PYY in portal plasma positively correlated with the concentration of SCFA in the cecal contents and cecal tissue weight. The concentrations of GLP-1 and PYY, in the altered diet group decreased to the same concentration as that measured in the FF group. However, it was low the similarities in the cecal bacteria population. Conclusion:The plasma concentrations of GLP-1 and PYY increased with increasing the amounts of SCFA, the products of DF fermentation, in the cecal contents. GLP-1 and PYY plasma concentrations and SCFA concentrations in the cecal contents rapidly responded to the altered diet, but the cecal bacteria population did not. Research ArticleOpen Access IntroductionA recent animal study showed that short-chain fatty acids (SCFA) produced by bacterial fermentation in the gut can directly influence L-cells to enhance the release of peptides such as glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) [1].The effect of SCFA in stimulating PYY and GLP-1 secretion from L-cells has also been demonstrated in humans [2]. GLP-1and PYY are cosecreted from endocrine L-cells that are found primarily in the distal gastrointestinal tract [3,4]. GLP-1 causes reduced appetite and increases the release of insulin [5]. PYY acts to reduce appetite [6]. PYY also acts in the stomach and intestine to slow down the movement of food through the digestive tract.Dietary fiber (DF) is indigestible carbohydrates that can be broken down by bacterial fermentation in the large intestine. Resistant starch (RS) is a type of starch that is not digested in the stomach or small intestine, reaching the large intestine intact. RS that reaches the large intestine can act as a substrate for microbial fermentation. The products of DF and RS fermentation are organic acids, mainly the SCFA acetate, propionate and butyrate, but also others such as succinate and lactate [7]. The difference in the am...

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Section: Profile Analysis Of Cecal Microbiota By Pcr-denaturing Gradimentioning

confidence: 99%

Plasma Concentrations of GLP-1 and PYY in Rats Fed Dietary Fiber Depend on the Fermentability of Dietary Fiber and Respond to an Altered Diet

Shimada¹,

Yoshimura²,

Murakami³

et al. 2015

Int J Clin Nutr Diet

View full text Add to dashboard Cite

show abstract

Investigation of Fecal Microbial Community Structure Using 16s rDNA Sequence in Pigs Fed Fermented Liquid Feed

Cited by 1 publication

References 18 publications

Plasma Concentrations of GLP-1 and PYY in Rats Fed Dietary Fiber Depend on the Fermentability of Dietary Fiber and Respond to an Altered Diet

Plasma Concentrations of GLP-1 and PYY in Rats Fed Dietary Fiber Depend on the Fermentability of Dietary Fiber and Respond to an Altered Diet

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