Investigation of fermentation conditions of biodiesel by-products for high production of β-farnesene by an engineered Escherichia coli

Yao, Pin; You, Shengping; Qi, Wei; Su, Rongxin; He, Zhimin

doi:10.1007/s11356-020-08893-z

Cited by 21 publications

(7 citation statements)

References 52 publications

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“…Several studies have engineered microorganisms for farnesene production [ 8 ]. The highest β-farnesene titer was approximately 10.31 g/L in Escherichia coli [ 10 ]. Using glucose as feedstock, the β-farnesene production reached 130 g/L in Saccharomyces cerevisiae [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Engineering Yarrowia lipolytica for the sustainable production of β-farnesene from waste oil feedstock

Liu

Zhang

et al. 2022

Biotechnol Biofuels

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Background β-Farnesene is a sesquiterpene with versatile industrial applications. The production of β-farnesene from waste lipid feedstock is an attractive method for sustainable production and recycling waste oil. Yarrowia lipolytica is an unconventional oleaginous yeast, which can use lipid feedstock and has great potential to synthesize acetyl-CoA-derived chemicals. Results In this study, we engineered Y. lipolytica to produce β-farnesene from lipid feedstock. To direct the flux of acetyl-CoA, which is generated from lipid β-oxidation, to β-farnesene synthesis, the mevalonate synthesis pathway was compartmentalized into peroxisomes. β-Farnesene production was then engineered by the protein engineering of β-farnesene synthase and pathway engineering. The regulation of lipid metabolism by enhancing β-oxidation and eliminating intracellular lipid synthesis was further performed to improve the β-farnesene synthesis. As a result, the final β-farnesene production with bio-engineering reached 35.2 g/L and 31.9 g/L using oleic acid and waste cooking oil, respectively, which are the highest β-farnesene titers reported in Y. lipolytica. Conclusions This study demonstrates that engineered Y. lipolytica could realize the sustainable production of value-added acetyl-CoA-derived chemicals from waste lipid feedstock.

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Section: Introductionmentioning

confidence: 99%

Engineering Yarrowia lipolytica for the sustainable production of β-farnesene from waste oil feedstock

Liu

Zhang

et al. 2022

Biotechnol Biofuels

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“…GC analysis indicated that α-farnesene production in strain SL08 increased up to 182.5 mg/L, which was approximately 3.0-fold higher than that in strain SL02 (Figure A). IDI1 (encoding IPP δ-isomerase) and ERG20 (encoding farnesyl diphosphate synthase) were key genes in the MVA pathway, which can be overexpressed to increase FPP supply and farnesene yield. , Therefore, IDI1 and ERG20 are cooverexpressed in strain WSL01 to obtain the recombinant strain WSL02. Furthermore, the plasmid pESC-CsAFS was transformed into strain WSL02 to generate strain SL09.…”

Section: Resultsmentioning

confidence: 99%

Enzyme and Metabolic Engineering Strategies for Biosynthesis of α-Farnesene in Saccharomyces cerevisiae

Qiao

Zhan

Nie

et al. 2023

J. Agric. Food Chem.

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α-Farnesene, a type of acyclic sesquiterpene, is an important raw material in agriculture, aircraft fuel, and the chemical industry. In this study, we constructed an efficient α-farnesene-producing yeast cell factory by combining enzyme and metabolic engineering strategies. First, we screened different plants for α-farnesene synthase (AFS) with the best activity and found that AFS from Camellia sinensis (CsAFS) exhibited the most efficient α-farnesene production in Saccharomyces cerevisiae 4741. Second, the metabolic flux of the mevalonate pathway was increased to improve the supply of the precursor farnesyl pyrophosphate. Third, inducing site-directed mutagenesis in CsAFS, the CsAFSW281C variant was obtained, which considerably increased α-farnesene production. Fourth, the N-terminal serine–lysine–isoleucine–lysine (SKIK) tag was introduced to construct the SKIK∼CsAFSW281C variant, which further increased α-farnesene production to 2.8 g/L in shake-flask cultures. Finally, the α-farnesene titer of 28.3 g/L in S. cerevisiae was obtained by fed-batch fermentation in a 5 L bioreactor.

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“…171 In one of the highest limonene titres reported, soluble AgGPPS levels did not correlate with titres, suggesting GPPS was not limiting. 171 IspA is the most common PPPS used in those studies reporting the highest obtained sesquiterpenoid titres 58,[172][173][174][175][176][177][178][179][180][181] (Table S1 †). IspA outperformed Blakeslea trispora and S. cerevisiae FPP synthase (FPPS) in direct comparisons.…”

Section: Alternative Prenyl Diphosphate Synthasesmentioning

confidence: 99%

Alternative metabolic pathways and strategies to high-titre terpenoid production inEscherichia coli

2022

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Investigation of fermentation conditions of biodiesel by-products for high production of β-farnesene by an engineered Escherichia coli

Cited by 21 publications

References 52 publications

Engineering Yarrowia lipolytica for the sustainable production of β-farnesene from waste oil feedstock

Engineering Yarrowia lipolytica for the sustainable production of β-farnesene from waste oil feedstock

Enzyme and Metabolic Engineering Strategies for Biosynthesis of α-Farnesene in Saccharomyces cerevisiae

Alternative metabolic pathways and strategies to high-titre terpenoid production inEscherichia coli

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