Investigation of Functionalized Poly(N,N‐dimethylacrylamide)‐block‐polystyrene Nanoparticles As Novel Drug Delivery System to Overcome the Blood–Brain Barrier In Vitro

Abstract

In the search of new drug delivery carriers for the brain, self-assembled nanoparticles (NP) were prepared from poly(N,N-dimethylacrylamide)-block-polystyrene polymer. NP displayed biocompatibility on cultured endothelial cells, macrophages and differentiated SH-SY5Y neuronal-like cells. The surface-functionalization of NP with a modified fragment of human Apolipoprotein E (mApoE) enhanced the uptake of NP by cultured human brain capillary endothelial cells, as assessed by confocal microscopy, and their permeability through a Transwell Blood Brain Barrier model made with the same cells, as assessed by fluorescence. Finally, mApoE-NP embedding doxorubicin displayed an enhanced release of drug at low pH, suggesting the potential use of these NP for the treatment of brain tumors.

Functionalized liposomes and phytosomes loading Annona muricata L. aqueous extract: Potential nanoshuttles for brain-delivery of phenolic compounds

“…4). Such result is already well established in the literature by several of our group's works concerning liposomes (Gobbi et al, 2010;Re et al, 2011;Bana et al, 2014) and polymeric nanocarriers (Gregori et al, 2015) as well as by other authors (Neves et al, 2016). Owing to the functionalization with mApoE peptide, transportermediated transport (namely receptor-mediated transcytosis) dominates for the nanoformulations (Re et al, 2011;Bana et al, 2014).…”

LincRNA-EPS in biomimetic vesicles targeting cerebral infarction promotes inflammatory resolution and neurogenesis

“…Our in vitro data confirmed that lncEPS was great for inflammatory inhibition and improving neuron regeneration. The barrier effect of the BBB is the main problem of improving therapeutic effects [45,46]. Moreover, drug targeting is the ideal solution to improve the therapeutic effects and reduce side effects.…”

Rapid Enkephalin Delivery Using Exosomes to Promote Neurons Recovery in Ischemic Stroke by Inhibiting Neuronal p53/Caspase-3

“…A suitable drug delivery system is the ideal solution to improve the therapeutic effects. Because of the BBB, few exosomes were transferred into the brain in the sham group and the tMCAO model [30, 31]. However, many vesicles were transferred into the brain after treatment with tar-exo.…”