Investigation of fungal contamination in medicinal and edible Lycii Fructus through DNA metabarcoding

Yu, Jingsheng; Jiang, Wenjun; Guo, Mengyue; Dao, Yujie; Pang, Xiaohui

doi:10.1111/jam.15662

Cited by 3 publications

(3 citation statements)

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“…Those findings are consistent with the results of our study ( Guo et al, 2018 ). Fungal contamination in Lycii Fructus was investigated via ITS2, and the results showed that Ascomycota, Dothideomycetes, Pleosporales, and Pleosporaceae predominated at various levels ranging from family to phylum ( Yu et al, 2022a ). In the Cassiae semen samples, Ascomycota was the prevailing fungus at the phylum level, and Aspergillus , Cladosporium and Penicillium were the most dominant genera ( Guo et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Sampling locations and processing methods shape fungi microbiome on the surface of edible and medicinal Arecae semen

Wei

Zhang

et al. 2023

Front. Microbiol.

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IntroductionArecae semen, which is derived from the dried ripe seed of Areca catechu L., has been commonly used as one of the major traditional Chinese medicines (TCMs). Three types of crude herbal preparations, namely, raw Arecae semen (AS), Arecae semen tostum (SAS), and Arecae semen carbonisata (FAS), are available for different clinical applications in TCMs. Although aflatoxin contamination in Arecae semen has been reported preliminarily, only a few studies have been conducted on fungal contamination.MethodsIn this study, the presence of fungi on the surface of three Arecae semen (AS, SAS, and FAS) that collected from four provinces were investigated using high-throughput sequencing and internal transcribed spacer 2.ResultsResults showed that the phyla Ascomycota (75.45%) and Basidiomycota (14.29%) and the genera Wallemia (7.56%), Botryosphaeria (6.91%), Davidiella (5.14%), and Symbiotaphrina (4.87%) were the dominant fungi, and they presented significant differences in four areas and three processed products (p < 0.05). The α-diversity and network complexity exhibited significant differences in the four sampling locations (p < 0.05), with higher in Yunnan (Chao 1, 213.45; Shannon, 4.61; average degree, 19.96) and Hainan (Chao 1, 198.27; Shannon, 4.21; average degree, 22.46) provinces. Significant differences were noted in the three processed samples; and SAS group had highest α-diversity (Chao 1, 167.80; Shannon, 4.54) and network complexity (average degree, 18.32).ConclusionsIn conclusion, the diversity and composition of microbiome on the surface of Arecae semen were shaped by sampling location and processing methods. This work provides details on the surface microbiome of Arecae semen samples and highlights the importance of roles of origin and processing methods in microbiomes, ensuring drug efficacy and food safety.

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Section: Discussionmentioning

confidence: 99%

Sampling locations and processing methods shape fungi microbiome on the surface of edible and medicinal Arecae semen

Wei

Zhang

et al. 2023

Front. Microbiol.

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“…[ 27 ] Due to the side effects of current anti-osteoporosis drugs, it is urgent to develop a more effective and safer treatment strategy. [ 28 ] Lycii Fructus, a common plant fruit with properties of drug homologous food, [ 10 ] has been widely used to treat kidney deficiency-induced bone diseases. Also, many studies showed its bone protect effects in the OVX animal model.…”

Section: Discussionmentioning

confidence: 99%

“…[ 9 ] Also, Lycii Fructus is widely used in home cooking such as tea, soups and porridge for its Chinese Pharmacopeia and pleasant flavor. [ 10 ] Lycii Fructus is used alone or mixed with other herbs to treat bone diseases for a long history. [ 11 - 13 ] Previous studies have demonstrated that Lycii Fructus has a bone protect effect and inhibits bone loss in ovariectomized (OVX) mice.…”

Section: Introductionmentioning

confidence: 99%

Network pharmacology-based pharmacological mechanism prediction of Lycii Fructus against postmenopausal osteoporosis

Wang,

et al. 2023

Medicine

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Postmenopausal osteoporosis (PMOP) has become one of most frequent bone diseases worldwide with aging population. Lycii Fructus, a common plant fruit with the property of drug homologous food, has long since been used to treat PMOP. The aim of this study is to explore pharmacological mechanisms of Lycii Fructus against PMOP through using network pharmacology approach. The active ingredients of Lycii Fructus were obtained from Traditional Chinese Medicine System Pharmacology database. Target fishing was performed on these ingredients in UniProt database for identification of the relative targets. Then, we screened the targets related to PMOP using GeneCards database and DisGeNET database. The overlapping genes between PMOP and Lycii Fructus were obtained to perform protein–protein interaction, gene ontology analysis, Kyoto Encyclopedia of Genes and Genomes analysis. A total of 35 active ingredients were identified in Lycii Fructus, and fished 158 related targets. Simultaneously, 292 targets associated with PMOP were obtained from GeneCards database and DisGeNET database. By drawing Venn diagram, 41 overlapping genes were obtained, and were considered as therapeutically relevant. Gene ontology enrichment analysis predicted that anti-inflammation and promotion of angiogenesis might be 2 potential mechanism of Lycii Fructus for PMOP treatment. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed several pathways, such as IL-17 pathway, TNF pathway, MAPK pathway, PI3K-Akt signaling pathway and HIF signaling pathway were involved in regulating these 2 biological processes. Through the method of network pharmacology, we systematically investigated the mechanisms of Lycii Fructus against PMOP. The identified multi-targets and multi-pathways provide new insights to further determinate its exact pharmacological mechanisms.

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Assembly and network of Rhei Radix et Rhizoma surface microbiome shaped by processing methods and sampling locations

Wei,

Chen,

Zhang

et al. 2024

Chinese Herbal Medicines

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Investigation of fungal contamination in medicinal and edible Lycii Fructus through DNA metabarcoding

Cited by 3 publications

References 58 publications

Sampling locations and processing methods shape fungi microbiome on the surface of edible and medicinal Arecae semen

Sampling locations and processing methods shape fungi microbiome on the surface of edible and medicinal Arecae semen

Network pharmacology-based pharmacological mechanism prediction of Lycii Fructus against postmenopausal osteoporosis

Assembly and network of Rhei Radix et Rhizoma surface microbiome shaped by processing methods and sampling locations

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