Investigation of growth kinetics of Debaryomyces hansenii (LAF‐3 10 U) in petroleum refinery desalter effluent

Abstract

Desalter effluent is a difficult to treat wastewater stream emerging from petroleum refining operations. In this study, an osmotolerant and halotolerant yeast, Debaryomyces hansenii, was investigated for the first time for its use in removing phenol from simulated and actual desalter effluent. D. hansenii was able to degrade phenols at substrate COD concentrations ranging from 300–1200 mg L−1, whereas the substrate inhibition was noted as COD increased from 2000–4000 mg L−1. Three kinetic models (Andrews, Aiba, and Edward) were applied as unstructured mathematical models to simulate substrate inhibition kinetics with respect to phenol COD. Among all models, the Edward model best simulated the yeast growth on phenol. Using the Edward model, the model parameters were μmax 0.21 h−1, KS 633.95 mg · L−1, and KI 1263.61 mg · L−1, respectively. The results in this study confirmed that D. hansenii has a high tolerance toward phenols and other organic compounds and salt found in desalter effluent. This yeast is therefore suggested to serve as an appropriate system from biological treatment of phenolic wastes in difficult to treat effluents such as desalter effluent.

Continuous cultivation of Debaryomyces hansenii (LAF‐3 10 U) on dodecane in synthetic desalter effluent at varying dilution rates on dodecane

“…The SDE in 1 L distilled water was prepared as described in Table 1. The amount of NaCl in Imperial Oil DE from Sarnia, ON, was measured by Maxam Laboratories at 1000 mg L −1 (Azimian et al, 2019); therefore, the same salt concentration was used in the SDE. The pH of all growth media was adjusted to range between 6 and 6.5.…”

“…D. hansenii is found in salty foods, blue cheese, nd brines, and it has both high respiratory and low fermentative activity (Calahorra et al, 2009; Şahin, 2017). This yeast was previously shown to remove phenols (aromatic compounds) (Azimian et al, 2019) and dodecane (an n‐alkane hydrocarbon) (García‐Lugo et al, 2018). Previous studies have shown that high substrate concentrations are inhibitory for yeast cultivation; therefore, continuous cultivation at a steady state in a continuous stirred‐tank reactor (CSTR) may be an effective way to control specific growth rates and avoid substrate inhibition.…”

Characterization and Development of Osmotolerant Caldicellulosiruptor Strains Targeting Enhanced Hydrogen Production from Lignocellulosic Hydrolysates

“…Inhibition arising from osmosensitivity can be one such phenomenon and is further addressed in this paper. This modeling of quantitative description of substrate inhibition and inhibition due to a high degree of osmotic stress have previously been studied using different types of growth kinetic equations (Azimian et al, 2019;Ciranna et al, 2014;Dötsch et al, 2008;van Niel et al, 2003). A non-competitive equation is commonly used to describe growth inhibition due to substrate or soluble end products (Ciranna et al, 2014;van Niel et al, 2003).…”

Response surface methodology (RMS) modelling to improve n‐dodecane degradation using Debaryomyces hanseniiLAF‐3 in a simulated desalter effluent

“…However, substantial dilution is required for conventional wastewater treatment due to the presence of salt, heavy metals, and other toxic compounds. [ 7 ] Petroleum refineries usually leave a high concentration of oil, in both miscible and immiscible forms, in the effluent. [ 8 ] Hence, different separation technologies have been developed to treat petroleum effluent; nevertheless, petroleum wastewater, after using primary and conventional separation technology, does not meet environmental limits.…”