Investigation of hemorheological parameters at the diagnosis and the follow-up of nutritional vitamin B12 deficient children

Abstract

We aimed to investigate the effects of vitamin B12 deficiency on hemorheological parameters, and the changes in these parameters following vitamin B12 treatment. 33 patients (mean-age:7 ± 5.7 years) diagnosed as nutritional vitamin B12 deficiency, and 31 age and sex matched controls (mean-age:7.1 ± 5.2 years) were enrolled. Erythrocyte deformability and aggregation were determined by an ectacytometer, plasma and whole blood viscosities by a cone-plate rotational viscometer. The differences between patients and controls were compared. Hemorheological parameters were repeated in the patient group following vitamin B12 treatment, and the results were compared with the initial results. In vitamin B12 deficiency, erythrocyte deformability and whole blood viscosity were found to be significantly decreased, eythrocyte aggregation was found to be significantly increased compared with the controls. Plasma viscosity was found to be decreased in deficiency but this decrease was not statistically significant. In patient group, erythrocyte deformability, whole blood and plasma viscosities were found to be significantly increased and erythrocyte aggregation was significantly decreased, after treatment. This study indicates that vitamin B12 deficiency has important effects on hemorheological parameters and adequate treatment of deficiency not only corrects the hematological parameters, but also by helping to normalize the hemorheological parameters, may contribute to the regulation of microvascular perfusion.

Effect of Age on Blood Rheology in Sickle Cell Anaemia and Sickle Cell Haemoglobin C Disease: A Cross-Sectional Study

“…a Blood viscosity values from Mackintosh et al [ 22 ]; 1D) Comparisons of RBC deformability measured at 3 Pa between AA, SS and SC patients at different age categories. bc RBC deformability values from Gurses et al [ 21 ] and Tancer-Elci et al [ 23 ]; 1E) Comparisons of RBC deformability measured at 30 Pa between AA, SS and SC patients at different age categories. bc RBC deformability values from Gurses et al [ 21 ] and Tancer-Elci et al [ 23 ]; 1F) Comparisons of RBC aggregates strength between AA, SS and SC patients at different age categories.…”

“…bc RBC deformability values from Gurses et al [ 21 ] and Tancer-Elci et al [ 23 ]; 1E) Comparisons of RBC deformability measured at 30 Pa between AA, SS and SC patients at different age categories. bc RBC deformability values from Gurses et al [ 21 ] and Tancer-Elci et al [ 23 ]; 1F) Comparisons of RBC aggregates strength between AA, SS and SC patients at different age categories. Difference between SS and SC (*p < 0.05; **p < 0.01; ***p < 0.001), SS and AA ( £ p < 0.05; £££ p < 0.001), SC and AA ( $$ p < 0.01; $$$ p < 0.001).…”

“…The number of individuals per age categories was: 0–3 (25 SS, 6 SC), 3–5 (15 SS, 6 SC), 5–10 (50 SS, 20 SC), 10–15 (56 SS, 34 SC), 15–20 (31 SS, 14 SC, 10 AA), 20–30 (27 SS, 20 SC, 11 AA), 30–40 (34 SS, 19 SC, 18 AA), 40–50 (15 SS, 12 SC, 23 AA), 50–60 (14 SS, 7 SC, 19 AA) and 60–70 (5 SC, 33 AA) yrs old. RBC deformability and blood viscosity values for healthy individuals younger than 15 yrs old were recorded from previous reports using the same devices as those used in the present study [ 21 , 22 , 23 ]. Analysis of variances with post-hoc Tukey test was used to compare the different parameters between the 3 populations across the different age categories.…”

Genetic and environmental factors associated with vitamin B12status in Amazonian children

“…In older children, other factors may be associated with vitamin B 12 deficiency, such as the absence of animal-derived foods or fortified foods ( 6 ) , a vegetarian diet ( 7 ) , low socio-economic level ( 8 ) and infection by gastrointestinal parasites ( 9 ) . Clinical presentations of vitamin B 12 deficiency include erythrocyte deformability ( 10 ) and neurological changes, which can occur in the absence of haematological abnormality ( 11 ) . The early diagnosis and treatment of vitamin B 12 deficiency in infants and children is important as long-term deficiency can cause developmental delay, failure to thrive, and clinical and neurological symptoms that can be irreversible ( 3 , 5 ) .…”