Investigation of human DNA profiles in house dust mites: Implications in forensic acarology

Çakan, Hüseyin; Güven, K. C.; Çevik, Filiz Ekim; Demırci, Mehmet; Saribas, Abdulkerim

doi:10.4323/rjlm.2015.187

Cited by 5 publications

(3 citation statements)

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“…Kester et al [136] suggested that with improvements, this method can have utility in general surveillance of human groups and verifying human activity within enclosed areas including covert sampling in homeland security cases. Dust mites, which feed on flakes of shed human skin, are another potential source of DNA for forensic analysis [137]. Approximately 2 g of dust was vacuum collected from each of the 27 houses, mites were isolated (9-190 mites/household) and DNA profiles generated using AmpF&STR MiniFiler kit.…”

Section: Human Dna In Dustmentioning

confidence: 99%

Emerging use of air eDNA and its application to forensic investigations – A review

Goray,

Taylor,

Bibbo

et al. 2024

Electrophoresis

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Biological material is routinely collected at crime scenes and from exhibits and is a key type of evidence during criminal investigations. Improvements in DNA technologies allow collection and profiling of trace samples, comprised of few cells, significantly expanding the types of exhibits targeted for DNA analysis to include touched surfaces. However, success rates from trace and touch DNA samples tend to be poorer compared to other biological materials such as blood. Simultaneously, there have been recent advances in the utility of environmental DNA collection (eDNA) in identification and tracking of different biological organisms and species from bacteria to naked mole rats in different environments, including, soil, ice, snow, air and aquatic. This paper examines the emerging methods and research into eDNA collection, with a special emphasis on the potential forensic applications of human DNA collection from air including challenges and further studies required to progress implementation.

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Section: Human Dna In Dustmentioning

confidence: 99%

Emerging use of air eDNA and its application to forensic investigations – A review

Goray,

Taylor,

Bibbo

et al. 2024

Electrophoresis

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“…In some cases, human DNA becomes concentrated inside other organisms that live in dust. For example, dust mites, which are abundant indoors in nearly all domestic settings [7,9] including the Russian space station [10] and feed on human skin cells present in dust, have also been identified as a suitable source of human DNA for forensic analysis [11]. The challenge in analyzing human DNA from dust, whether it is concentrated in mites or freefloating, is that it will often contain a mixture of DNA from mulitple individuals [8,11].…”

Section: Introductionmentioning

confidence: 99%

“…For example, dust mites, which are abundant indoors in nearly all domestic settings [7,9] including the Russian space station [10] and feed on human skin cells present in dust, have also been identified as a suitable source of human DNA for forensic analysis [11]. The challenge in analyzing human DNA from dust, whether it is concentrated in mites or freefloating, is that it will often contain a mixture of DNA from mulitple individuals [8,11]. As a result, typed alleles would require deconvolution into single individual profiles for downstream comparison to an evidence profile or the Combined DNA Index System (CODIS).…”

Section: Introductionmentioning

confidence: 99%

Using FastID to analyze complex SNP mixtures from indoor dust

Meiklejohn

Scheible

Boggs

et al. 2023

Journal of Forensic Sciences

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Forensically relevant single nucleotide polymorphisms (SNPs) can provide valuable supplemental information to short tandem repeats (STRs) for investigative leads, and genotyping can now be streamlined using massively parallel sequencing (MPS). Dust is an attractive evidence source, as it accumulates on undisturbed surfaces, often is overlooked by perpetrators, and contains sufficient human DNA for analysis. To assess whether SNPs genotyped from indoor dust using MPS could be used to detect known household occupants, 13 households were recruited and provided buccal samples from each occupant and dust from five predefined indoor locations. Thermo Fisher Scientific Precision ID Identity and Ancestry Panels were utilized for SNP genotyping, and sequencing was completed using Illumina® chemistry. FastID, a software developed to permit mixture analysis and identity searching, was used to assess whether known occupants could be detected from associated household dust samples. A modified “subtraction” method was also used in FastID to estimate the percentage of alleles in each dust sample contributed by known and unknown occupants. On average, 72% of autosomal SNPs were recovered from dust samples. When using FastID, (a) 93% of known occupants were detected in at least one indoor dust sample and could not be excluded as contributors to the mixture, and (b) non‐contributor alleles were detected in 54% of dust samples (29 ± 11 alleles per dust sample). Overall, this study highlights the potential of analyzing human DNA present in indoor dust to detect known household occupants, which could be valuable for investigative leads.

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A systematic approach to the analysis of illicit drugs for DNA with an overview of the problems encountered

Griffin,

Kirkbride,

Painter

et al. 2024

Forensic Science International

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Investigation of human DNA profiles in house dust mites: Implications in forensic acarology

Cited by 5 publications

References 0 publications

Emerging use of air eDNA and its application to forensic investigations – A review

Emerging use of air eDNA and its application to forensic investigations – A review

Using FastID to analyze complex SNP mixtures from indoor dust

A systematic approach to the analysis of illicit drugs for DNA with an overview of the problems encountered

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